PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0325 [new locus tag: SAUSA300_RS01730 ]
• pan locus tag^?: SAUPAN001856000
• symbol: SAUSA300_0325
• pan gene symbol^?: gcvH-L
• synonym:
• product: glycine cleavage H-protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0325 [new locus tag: SAUSA300_RS01730 ]
• symbol: SAUSA300_0325
• product: glycine cleavage H-protein
• replicon: chromosome
• strand: +
• coordinates: 378165..378497
• length: 333
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913721 NCBI
• RefSeq: YP_493039 NCBI
• BioCyc: see SAUSA300_RS01730
• MicrobesOnline: 1291840 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
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  301
  
  ATGAAAAAGTTAGCCAATTATTTATGGGTAGAAAAAGTAGGAGATTTGTATGTGTTTAGT
  ATGACACCTGAATTGCAAGATGATATTGGGACAGTAGGTTATGTTGAATTCGTAAGTCCA
  GATGAAGTTAAAGTGGATGATGAAATTGTGAGTATCGAAGCATCGAAAACGGTCATTGAT
  GTGCAAACGCCATTGTCAGGAACGATTATTGAGCGAAATACAAAAGCGGAAGAAGAACCG
  ACAATTTTAAACTCTGAAAAACCAGAAGAAAATTGGTTGTTCAAATTGGATGATGTCGAT
  AAAGAAGCATTCCTAGCATTACCGGAGGCTTAA
  60
  120
  180
  240
  300
  333
  

⊟Protein

⊟General

• locus tag: SAUSA300_0325 [new locus tag: SAUSA300_RS01730 ]
• symbol: SAUSA300_0325
• description: glycine cleavage H-protein
• length: 110
• theoretical pI: 3.86736
• theoretical MW: 12421.9
• GRAVY: -0.263636

⊟Function

• TIGRFAM:
  Metabolism Energy metabolism Amino acids and amines glycine cleavage system H protein (TIGR00527; HMM-score: 57.2)
  and 3 more
  glycine cleavage protein H-like protein (TIGR03077; HMM-score: 38.6)
  Metabolism Energy metabolism TCA cycle dihydrolipoyllysine-residue succinyltransferase, E2 component of oxoglutarate dehydrogenase (succinyl-transferring) complex (TIGR01347; EC 2.3.1.61; HMM-score: 18.3)
  Metabolism Central intermediary metabolism Nitrogen metabolism urea carboxylase (TIGR02712; EC 6.3.4.6; HMM-score: 9.4)
• TheSEED  :
  • Biotinyl-lipoyl attachment domain protein, GcvH-like
• PFAM:
  Hybrid (CL0105) GCV_H; Glycine cleavage H-protein (PF01597; HMM-score: 61.4)
  and 1 more
  Biotin_lipoyl; Biotin-requiring enzyme (PF00364; HMM-score: 19.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010765
  • TAT(Tat/SPI): 0.000132
  • LIPO(Sec/SPII): 0.000825
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161132 NCBI
• RefSeq: YP_493039 NCBI
• UniProt: A0A0H2XG03 UniProt

⊟Protein sequence

• MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVKVDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKEAFLALPEA

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0324 > SAUSA300_0325 > SAUSA300_0326 > SAUSA300_0327 > SAUSA300_0328

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Johannes Gregor Matthias Rack, Rosa Morra, Eva Barkauskaite, Rolf Kraehenbuehl, Antonio Ariza, Yue Qu, Mary Ortmayer, Orsolya Leidecker, David R Cameron, Ivan Matic, Anton Y Peleg, David Leys, Ana Traven, Ivan Ahel
Identification of a Class of Protein ADP-Ribosylating Sirtuins in Microbial Pathogens.
Mol Cell: 2015, 59(2);309-20
[PubMed:26166706] [WorldCat.org] [DOI] (I p)