From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0333 [new locus tag: SAUSA300_RS01770 ]
  • pan locus tag?: SAUPAN001866000
  • symbol: SAUSA300_0333
  • pan gene symbol?: —
  • synonym:
  • product: BglG family transcriptional antiterminator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0333 [new locus tag: SAUSA300_RS01770 ]
  • symbol: SAUSA300_0333
  • product: BglG family transcriptional antiterminator
  • replicon: chromosome
  • strand: -
  • coordinates: 384760..386715
  • length: 1956
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    TTGTTAAGTTATAGACAAATCGAAATTTTGTATCTTTTAATTCATGAGCAAACGTTTATT
    CCAATCAATACCATTGCTAATCAACTTGGTGTATCACCTCGTACCATTCAATATGATATA
    GCATATATTGAGCAATATGCTGAAACTTATCATTACCAAGTCAGCCGTAATAAAGCTGCA
    GGCATCAAAGTAACAACAGCTCACGCGACACTATTAAATGAATTAGAGCACAATCTAACA
    AATCAAATTCATTTTTCAAAAGATGAACGACTCACACATATCGCTTTAAAGTTATTCGAA
    ACAACCGATCCTGTTTCAACAAAGCAACTTGCGCAAGATGTTAATGTTTCGCGTCGGACA
    ATTGCAGATGATATTAAAATGATTCAAGCCCAACTAGATCAATACCATTTAAAGTTAAAT
    TATGTCCACAATAAAGGATTCAATATTATTGGTGAGGAAGATCATTATCGTAAAGCGTAT
    GCACACTTTATACATCAATATATGAAACAAGCTGCACCTTTTATAGAGGCGGATATCTTT
    AATTCAGAATCAATCGCATTGGTTCGCCGTGCCATTATTAAGACATTAAATAGTGAAAAT
    TATCATTTAGTTCAGTCGGCTATCGATGGCTTAATCTATCATATACTCATTGCCATTCAG
    CGTTTAAATGAAAATTTTTCGTTCGATATACCTATCAATGAAATTGATAAATGGCGACAT
    ACTAATCAGTATGCTATTGCTTCAAAAATGATAGAAAACTTAGAACGCAGTTGTAATGTC
    ACATTTCCAGAGTCTGAAATTATCTTTATCACGTTACATTTACTAGGCTCTAAAATGACT
    GAACATACAGCATCTTCAATTACCTTTGAATACCATGATTTATCGCAAAATATACATGAA
    TTGATCACTTGTGTTAGCCAAGAATTAGGCATTGATATGTCAAAAGACAACAAGTTACAT
    ACCAGTCTGATCACACATATCAAACCAGCTATACATCGTATTAAATACGATATGCTACAA
    CCTAATCCTTTGAGGCAAGAAGTTATGCGTCGCTATCCTCAAATCATTGAAGCCGTTAGC
    AAGCATATTAGTCCAATTGAACAAGATGCTGCTATTCGCTTCAACGAAGATGAATTAACA
    TACATTACAATTCACTTCGCATCAAGTATAGAGCGTGTTGCAACACATAAACAATCAATG
    ATTAAGGTTGTCTTACTATGTGGTTCTGGTATAGGCACGTCACAACTTTTAAAATCAAAA
    CTAAATCACCTGTATCCTGAGTTTCACATTTGGGATGCCTATTCCATTTATCAATTGGAA
    GAAAGTCGATTATTGCAAGATAACATTGATTATGTCATTTCAACAGTACCTTGTGAAATA
    TCAGCTGTACCAGTTATTCATGTCGATCCATTTATCAATCAACAATCTCGTCAAAAATTG
    AATCAAATTATCAATGACTCAAGAGAACAACGAGTCATGAAAATGGCAACTGATGGCAAG
    TCACTCGCAGATTTATTGCCTGAACATCGCATCATTATAAATAAACAACCATTATCAATT
    GAATCCGCAATTGCAGTGGCTGTGCAACCTTTAATCAATGATGGCATTGTCTATTCAAAT
    TATACAGCTGCAATTTTAAAACAATTTGAACAATTCGGGTCATATATGGTCATTAGTCCA
    CATATTGCACTTATTCACGCTGGTACTGATTATGTACAGAATGGTGTAGGTTTCGCACTA
    ACATATTTCACTGAAGGGATTATCTTTGGTAGTAAAGCTAACGATCCCGTTCACCTTGTA
    ATTACATTAGCAACGGACCACCCCAATGCACATTTAAAGGCATTGGGACAGTTAAGCGAA
    TGCTTAAGCAACGACTTATATCGACAAGATTTCTTAGATGGGAATATTTTTAAAATTAAA
    CAACACATTGCTTTAACTATGACAAAGGAGGCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1956

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0333 [new locus tag: SAUSA300_RS01770 ]
  • symbol: SAUSA300_0333
  • description: BglG family transcriptional antiterminator
  • length: 651
  • theoretical pI: 6.60796
  • theoretical MW: 74472.7
  • GRAVY: -0.156221

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, fructose subfamily, IIA component (TIGR00848; EC 2.7.1.69; HMM-score: 27.5)
    Signal transduction Signal transduction PTS PTS system, fructose subfamily, IIA component (TIGR00848; EC 2.7.1.69; HMM-score: 27.5)
    and 1 more
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 13.9)
  • TheSEED  :
    • PTS system, mannitol/fructose-specific IIA component (EC 2.7.1.69)
    • Putative transcriptional antiterminator, BglG family
  • PFAM:
    PRD (CL0166) PRD; PRD domain (PF00874; HMM-score: 119.5)
    and 22 more
    PTase-anion_tr (CL0340) PTS_EIIA_2; Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2 (PF00359; HMM-score: 72.5)
    HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 65.9)
    Mga; Mga helix-turn-helix domain (PF05043; HMM-score: 45.4)
    HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 30.8)
    HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 25.5)
    GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 25.1)
    PuR_N; Bacterial purine repressor, N-terminal (PF09182; HMM-score: 24)
    Phosphatase (CL0031) PTS_IIB; PTS system, Lactose/Cellobiose specific IIB subunit (PF02302; HMM-score: 21.5)
    HTH (CL0123) Sigma70_ECF; ECF sigma factor (PF07638; HMM-score: 18)
    HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 17.2)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 17.1)
    UPF0122; Putative helix-turn-helix protein, YlxM / p13 like (PF04297; HMM-score: 17)
    HTH_10; HTH DNA binding domain (PF04967; HMM-score: 16.9)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 16.7)
    Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 16.3)
    HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 16.1)
    HTH_29; Winged helix-turn helix (PF13551; HMM-score: 14.5)
    HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 13.9)
    HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 13.7)
    MerR; MerR family regulatory protein (PF00376; HMM-score: 13.3)
    HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 12.4)
    HTH_AsnC-type; AsnC-type helix-turn-helix domain (PF13404; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.028371
    • TAT(Tat/SPI): 0.000909
    • LIPO(Sec/SPII): 0.001981
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIEQYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETTDPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAYAHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIAIQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLGSKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRIKYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIERVATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNIDYVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPEHRIIINKQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIHAGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSNDLYRQDFLDGNIFKIKQHIALTMTKEA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_0333&oldid=53513"