PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0430 [new locus tag: SAUSA300_RS02305 ]
• pan locus tag^?: SAUPAN002170000
• symbol: SAUSA300_0430
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0430 [new locus tag: SAUSA300_RS02305 ]
• symbol: SAUSA300_0430
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 483702..484436
• length: 735
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914638 NCBI
• RefSeq: YP_493143 NCBI
• BioCyc: see SAUSA300_RS02305
• MicrobesOnline: 1291945 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAGAATTAAAACACCGAGTCCATCGTATTTAAAAGGCACAAATGGACATGCGATATTA
  TTATTACATTCATTTACAGGTACAAATCGGGATGTGAAGCATCTTGCAGCTGAGTTAAAT
  GACCAAGGATTTAGTTGTTATGCACCGAATTATCCAGGTCATGGTTTATTGTTGAAAGAT
  TTCATGACATATAATGTAGATGATTGGTGGGAAGAAGTTGAGAAAGCTTACCAATTTTTA
  GTCAATGAAGGTTATGAATCTATCAGTGCAACGGGTGTGTCTTTAGGTGGATTAATGACA
  TTAAAATTGGCGCAACACTATCCTTTGAAACGTATCGCTGTCATGTCAGCACCAAAGGAA
  AAGAGTGACGATGGCTTAATAGAACATTTAGTTTATTATAGTCAACGCATGTCGAATATT
  TTAAATTTAGATCAGCAAGCATCGAGTGCGCAATTAGCAGCAATTGATGATTATGAAGGT
  GAAATTACGAAGTTTCAACATTTTATTGATGATATCATGACAAATTTAAATGTTATTAAA
  ATGCCAGCTAATATATTATTTGGTGGTAAAGATGCGCCATCCTATGAAACAAGTGCACAT
  TTTATTTATGAACATTTAGGATCAGTAGACAAAGAATTAAATGGTCTGAAGGATTCGCAT
  CATTTAATGACGCATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTC
  AATGCTTTAACATAA
  60
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  735
  

⊟Protein

⊟General

• locus tag: SAUSA300_0430 [new locus tag: SAUSA300_RS02305 ]
• symbol: SAUSA300_0430
• description: hypothetical protein
• length: 244
• theoretical pI: 5.48333
• theoretical MW: 27442.8
• GRAVY: -0.312295

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 31.9)
  and 6 more
  Metabolism Energy metabolism Other 3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 22.2)
  exosortase A system-associated hydrolase 2 (TIGR03101; HMM-score: 20.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin pimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 20.5)
  Metabolism Energy metabolism Photosynthesis putative magnesium chelatase accessory protein (TIGR03056; HMM-score: 17.3)
  cyanobacterial long protein repeat (TIGR02059; HMM-score: 16.8)
  Plasmodium subtelomeric family (TIGR01607; HMM-score: 12.2)
• TheSEED  :
  • Esterase/lipase family protein
• PFAM:
  AB_hydrolase (CL0028) Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 55.7)
  and 7 more
  Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 29.4)
  Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 19.8)
  DLH; Dienelactone hydrolase family (PF01738; HMM-score: 17.8)
  UPF0227; Uncharacterised protein family (UPF0227) (PF05728; HMM-score: 14.3)
  Esterase; Putative esterase (PF00756; HMM-score: 13.2)
  Peptidase_S9; Prolyl oligopeptidase family (PF00326; HMM-score: 12.3)
  DUF2048; Abhydrolase domain containing 18 (PF09752; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.129448
  • TAT(Tat/SPI): 0.018088
  • LIPO(Sec/SPII): 0.025088
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162095 NCBI
• RefSeq: YP_493143 NCBI
• UniProt: A0A0H2XI47 UniProt

⊟Protein sequence

• MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGFSCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLKLAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEGEITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKDSHHLMTHGEGRDILEENVIRFFNALT

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0429 > SAUSA300_0430

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications