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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0516 [new locus tag: SAUSA300_RS02765 ]
  • pan locus tag?: SAUPAN002299000
  • symbol: SAUSA300_0516
  • pan gene symbol?: mrnC
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0516 [new locus tag: SAUSA300_RS02765 ]
  • symbol: SAUSA300_0516
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 578481..578885
  • length: 405
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    GTGGATAATCAACAAGATAATCACATTAAATTATTGAATCCATTGACCTTAGCATATATG
    GGAGACGCAGTCTTAGATCAATATGTACGTACCTATATCGTTTTAAAGCTTAAAAGTAAG
    CCTAATAAACTACATCAAATGTCTAAAAAATATGTATCTGCCAAAAGTCAGGCGCAAACG
    TTAGAATATTTAATGGAGCAAGAATGGTTTACAGACGAAGAAATGGATATTTTGAAGCGA
    GGGCGTAACGCGAAAAGTCATACTAAAGCTAAAAACACTGATGTTCAAACATATCGTAAA
    AGTTCAGCGATAGAAGCAGTGATAGGTTTTCTTTATTTAGAAAAAAGAGAAGAACGATTA
    GAGGCATTATTAAATAAAATAATAACAATAGTAAACGAAAGGTAG
    60
    120
    180
    240
    300
    360
    405

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0516 [new locus tag: SAUSA300_RS02765 ]
  • symbol: SAUSA300_0516
  • description: hypothetical protein
  • length: 134
  • theoretical pI: 9.69436
  • theoretical MW: 15721
  • GRAVY: -0.647015

Function[edit | edit source]

  • reaction:
    EC 3.1.26.-?  ExPASy
  • TIGRFAM:
  • TheSEED  :
    • Mini-ribonuclease III
  • PFAM:
    RNase_III (CL0539) Ribonuclease_3; Ribonuclease III domain (PF00636; HMM-score: 66.2)
    and 2 more
    Ribonucleas_3_3; Ribonuclease-III-like (PF14622; HMM-score: 21.9)
    no clan defined DUF4654; Domain of unknown function (DUF4654) (PF15547; HMM-score: 13.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004242
    • TAT(Tat/SPI): 0.000198
    • LIPO(Sec/SPII): 0.000671
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKLHQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSSAIEAVIGFLYLEKREERLEALLNKIITIVNER

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]