PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0536 [new locus tag: SAUSA300_RS02865 ]
• pan locus tag^?: SAUPAN002323000
• symbol: SAUSA300_0536
• pan gene symbol^?: hchA
• synonym:
• product: chaperone protein HchA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0536 [new locus tag: SAUSA300_RS02865 ]
• symbol: SAUSA300_0536
• product: chaperone protein HchA
• replicon: chromosome
• strand: +
• coordinates: 600990..601868
• length: 879
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914160 NCBI
• RefSeq: YP_493239 NCBI
• BioCyc: see SAUSA300_RS02865
• MicrobesOnline: 1292051 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGTCACAAGATGTAAATGAATTAAGTAAGCAACCAACGCCAGATAAAGCAGAAGATAAC
  GCATTTTTCCCATCACCATATTCCCTTAGTCAATATACAGCACCTAAAACAGATTTTGAT
  GGTGTTGAACACAAAGGTGCCTATAAAGATGGTAAATGGAAAGTATTGATGATTGCTGCT
  GAAGAGCGATATGTATTATTGGAAAATGGAAAAATGTTCTCTACGGGTAATCATCCTGTT
  GAAATGTTATTACCTTTACATCATTTAATGGAAGCAGGTTTTGACGTTGATGTTGCGACA
  TTATCTGGTTATCCAGTTAAATTAGAATTATGGGCTATGCCAACTGAAGACGAGGCAGTT
  ATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCCAAAAAAATTAGCAGATGTG
  ATTAAAAATGAATTAGGACCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACAT
  GCTGCAGTTGTTGGTATTTCTGAAAGTGAGGACGTTCAACAAACATTAGATTGGGCATTA
  GACAATGACCGCTTTATAGTTACATTATGTCATGGACCAGCAGCACTACTTTCAGCAGGG
  CTTAACAGAGAAAAATCTCCATTAGAAGGATACTCTGTTTGTGTCTTCCCTGACTCATTA
  GATGAAGGTGCAAATATTGAAATAGGTTATTTACCTGGACGCTTGAAATGGTTAGTTGCT
  GATTTATTAACTAAACAAGGATTAAAAGTAGTTAACGACGATATGACAGGAAGAACGTTA
  AAAGATCGTAAATTATTAACAGGTGACAGTCCTTTAGCTTCAAATGAGTTAGGAAAATTA
  GCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAA
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⊟Protein

• 5XR2
  PDB
• 5XR3
  PDB

Protein Data Bank: 5XR2

Protein Data Bank: 5XR3

⊟General

• locus tag: SAUSA300_0536 [new locus tag: SAUSA300_RS02865 ]
• symbol: SAUSA300_0536
• description: chaperone protein HchA
• length: 292
• theoretical pI: 4.65565
• theoretical MW: 32176.4
• GRAVY: -0.307877

⊟Function

• reaction:
  EC 3.5.1.124?  ExPASy
  Protein deglycase An N(omega)-(1-hydroxy-2-oxopropyl)-[protein]-L-arginine + H₂O = a [protein]-L-arginine + (R)-lactate An N⁶-(1-hydroxy-2-oxopropyl)-[protein]-L-lysine + H₂O = a [protein]-L-lysine + (R)-lactate An S-(1-hydroxy-2-oxopropyl)-[protein]-L-cysteine + H₂O = a [protein]-L-cysteine + (R)-lactate
  EC 3.1.2.-?  ExPASy
• TIGRFAM:
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides intracellular protease, PfpI family (TIGR01382; HMM-score: 28.8)
  and 1 more
  Unknown function General DJ-1 family protein (TIGR01383; HMM-score: 17.5)
• TheSEED  :
  • D-lactate dehydratase (EC 4.2.1.130)
• PFAM:
  Glutaminase_I (CL0014) DJ-1_PfpI; DJ-1/PfpI family (PF01965; HMM-score: 38.6)
  and 2 more
  ThiJ_like; ThiJ/PfpI family-like (PF17124; HMM-score: 20.6)
  RND_permease (CL0322) ACR_tran; AcrB/AcrD/AcrF family (PF00873; HMM-score: 9.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006928
  • TAT(Tat/SPI): 0.001034
  • LIPO(Sec/SPII): 0.001259
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161390 NCBI
• RefSeq: YP_493239 NCBI
• UniProt: Q2FJ89 UniProt

⊟Protein sequence

• MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEHKGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLSGYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGHAAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPDSLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNELGKLAVNEMLNAIQNK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications