PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0617 [new locus tag: SAUSA300_RS03310 ]
• pan locus tag^?: SAUPAN002502000
• symbol: SAUSA300_0617
• pan gene symbol^?: —
• synonym:
• product: Na+/H+ antiporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0617 [new locus tag: SAUSA300_RS03310 ]
• symbol: SAUSA300_0617
• product: Na+/H+ antiporter
• replicon: chromosome
• strand: +
• coordinates: 688348..690351
• length: 2004
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913817 NCBI
• RefSeq: YP_493320 NCBI
• BioCyc: see SAUSA300_RS03310
• MicrobesOnline: 1292132 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  GTGATACTAAGTTCGTTTGTCCATACTTTCATACCTAAAGTACCCCTAGCATTTATACAA
  ATTTTCTTGGGCATGTTACTATTTATTACCCCAATCCCTGTTCAATTTAATTTTGATTCT
  GAATTGTTTATGGTAACAATGATTGCGCCTTTGTTATTTGTAGAAGGTGTTAATGTTTCT
  AGAGTCCATTTAAGGAAATATATTAAGCCAGTGATGATGATGGCATTAGGATTAGTCATT
  ACTACTGTGATAGGTGTAGGTTTATTTATTCATTGGATTTGGCCAGATTTACCTATTGGA
  GCAGCATTTGCAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATC
  ACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTG
  AATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCT
  TTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGTGGTT
  GGTTTACTTATAGGTATGGCATTAGTAAGGTTCCGATTAACATTGATGCGTCGAGGATAT
  GAAAACATTAATATGTTTACAATTATTCAATTGTTAACACCATTTGTTACGTATTTAATT
  GCTGAATTGTTTCACGCATCAGGAATCATTGCAGCAGTAGTTGCAGGACTTGTACATGGT
  TTCGAACGTGACAGAATTATGCAAGTACGTACACAACTGCAAATGAGTTACAATCATACA
  TGGAATATACTAGGTTATGTTTTAAATGGCTTTGTTTTTTCAATATTAGGATTTTTAGTA
  CCTGAAGTTATTATTAAAATTATCAAAACAGAACCGCACAATTTAATCTTTTTAATAGGC
  ATCACTATTGTTGTTGCTTTAGCTGTCTATCTATTTAGATTTGTTTGGGTTTATGTCTTA
  TATCCTTATTTTTATTTAGCCATCAGTCCATTCCAAAAAATGATGACTAAAAATGATGAT
  GATAATCCAACGACTGAGAAACCACCAAAGCGAAGTTTATACGCTTTAATTATGACGTTA
  TGTGGTGTGCATGGAACAATTTCTTTAGCAATCGCATTAACGTTACCGTATTTTTTAGCA
  GGGCATCATGCTTTTACGTATAGAAACGACTTATTATTTATTGCATCTGGTATGGTTATT
  ATTAGTTTGGTAGTTGCGCAAGTATTATTGCCATTATTAACGAAACCTGCACCTAAAACA
  GTAATTGGCAATATGTCGTTTAAAGTTGCTAGAATTTATATATTAGAACAAGTTATTGAT
  TATCTAAATCAAAAATCTACTTTCGAAACAAGTTTTAAATATGGTAACGTGATTAAAGAA
  TATCATGATAAATTAGCATTTTTAAAAACTGTAGAGAAAGATGATGAAAACTCTAAAGAA
  TTAGAACGTCTACAAAAAATTGCTTTTAATGTAGAAACAAAAACATTAGAGTCTTTAGTA
  GATGAAGGACAAATAACGAATAGTGTACTTGAAAACTATATGCGTTATGCTGAAAGAACA
  CAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTATTATTACGAGGTGCTTTA
  TTAAAACGAAGAGTACAAACGAGAGTGAACTCCGCATCTTCACTTAGTGTTACGGATAAC
  TTAATGGAATTAAATAAAATTAATAAATTAGTCCATTATAATGTGGTTAGTCGTTTGTCT
  AAGGAAACAACAAAAGATAATACACTTGAAGTTGGAATGGTTTGTGACGGTTATTTAATG
  CGAATTGAAAACTTAACACCATCAAATTTCTTCAACTCAGCAAGTGAAGATACGATTACT
  AAAATTAAATTAAATGCATTGAGAGAACAACGTCGCATTTTACGTGAGTTGATTGATACA
  GATGAAGTATCAGAAGGTACAGCGTTAAAACTAAGAGAAGCCATCAATTATGATGAAATG
  GTTATTGTAGATAGTATGACGTAG
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⊟Protein

⊟General

• locus tag: SAUSA300_0617 [new locus tag: SAUSA300_RS03310 ]
• symbol: SAUSA300_0617
• description: Na+/H+ antiporter
• length: 667
• theoretical pI: 9.23772
• theoretical MW: 74934.3
• GRAVY: 0.471664

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds Na+/H+ antiporter (TIGR00831; HMM-score: 258)
  and 2 more
  Metabolism Transport and binding proteins Cations and iron carrying compounds sodium/hydrogen exchanger 3 (TIGR00840; HMM-score: 52.1)
  Metabolism Transport and binding proteins Cations and iron carrying compounds sodium/hydrogen antiporter (TIGR00844; HMM-score: 7.3)
• TheSEED  :
  • Na+/H+ antiporter
• PFAM:
  CPA_AT (CL0064) Na_H_Exchanger; Sodium/hydrogen exchanger family (PF00999; HMM-score: 173.4)
  and 1 more
  no clan defined Trans_coact; Phage late-transcription coactivator (PF16805; HMM-score: 14.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.03607
  • TAT(Tat/SPI): 0.000535
  • LIPO(Sec/SPII): 0.007283
• predicted transmembrane helices (TMHMM): 13

⊟Accession numbers

• GI: 87162333 NCBI
• RefSeq: YP_493320 NCBI
• UniProt: A0A0H2XKF9 UniProt

⊟Protein sequence

• MILSSFVHTFIPKVPLAFIQIFLGMLLFITPIPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLFIHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGIISFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINMFTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNILGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLYPYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFLAGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQVIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKTLESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSASSLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFFNSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications