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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0619 [new locus tag: SAUSA300_RS03320 ]
  • pan locus tag?: SAUPAN002508000
  • symbol: SAUSA300_0619
  • pan gene symbol?: mntB
  • synonym:
  • product: ABC transporter permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0619 [new locus tag: SAUSA300_RS03320 ]
  • symbol: SAUSA300_0619
  • product: ABC transporter permease
  • replicon: chromosome
  • strand: -
  • coordinates: 692437..693273
  • length: 837
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGTTAGAGTTTGTCGAACATTTATTTACATATCAATTCTTGAATCGAGCATTGATAACT
    TCAATTATTGTAGGGATAGTTTGTGGTACAGTTGGTAGTTTAATTGTATTACGTGGTCTT
    TCATTAATGGGAGATGCAATGAGTCACGCAGTATTACCTGGTGTTGCCCTATCATTCTTA
    TTTGGTATTCCAATGTTTGTAGGCGCACTTATAACTGGTATGATCGCAAGTATTTTTATC
    GGTTATATCACATCTAGTAGTAAAACGAAACCTGACGCCGCTATTGGAATTAGTTTTACC
    GCTTTCTTAGCTTCGGGGATTATTATTATTAGCTTAATAAACACTACGACAGATTTGTAC
    CATATTCTGTTTGGTAATTTACTGGCAATCACAAATAGTGCATTTTTAACAACTATTGTG
    ATTGGTTCAATCGTCCTTATTCTAATCATTATTTTCTATCGTCCATTAATGATTTCTACA
    TTCGATCCAACGTTTAGTAGAATGAGTGGTCTAAATACGACGTTATTACATTACTTTGTG
    ATGTTGTTACTCTCATTAGTAACAGTAGCAAGTATTCAAACGGTAGGTATTATTCTTGTA
    GTTGCATTATTAATTACACCTGCATCTACAGCATTTTTAATTAGTAAAAAACTATACTCA
    ATGATGATTATAGCAAGTTTAATAAGTGTCATAAGTTCAATTGTCGGACTTTATTACAGC
    TATATTTACAACATTCCTAGTGGTGCGACAATCGTACTTTGTACGTTTGTAATTTATATC
    ATCACATTATTTTTCACAAAATTTACGAATAGAAAGAAACGAGGAAGTTTAACATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    837

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0619 [new locus tag: SAUSA300_RS03320 ]
  • symbol: SAUSA300_0619
  • description: ABC transporter permease
  • length: 278
  • theoretical pI: 9.77742
  • theoretical MW: 30104.2
  • GRAVY: 1.25108

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Unknown substrate anchored repeat-type ABC transporter, permease subunit (TIGR03770; HMM-score: 182.1)
  • TheSEED  :
    • Manganese/zinc ABC transporter (EC 7.2.2.5), permease protein ScaB
  • PFAM:
    Membrane_trans (CL0142) ABC-3; ABC 3 transport family (PF00950; HMM-score: 260.6)
    and 3 more
    FecCD; FecCD transport family (PF01032; HMM-score: 27.6)
    no clan defined YccF; Inner membrane component domain (PF03733; HMM-score: 12.4)
    DUF2407_C; DUF2407 C-terminal domain (PF13373; HMM-score: 11)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007622
    • TAT(Tat/SPI): 0.001273
    • LIPO(Sec/SPII): 0.024432
  • predicted transmembrane helices (TMHMM): 8

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMGDAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAFLASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMISTFDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKLYSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGSLT

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: MntR* (repression) regulon
    MntR*(TF)important in Manganese homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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