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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0631 [new locus tag: SAUSA300_RS03380 ]
  • pan locus tag?: SAUPAN002522000
  • symbol: SAUSA300_0631
  • pan gene symbol?: nupG
  • synonym:
  • product: putative nucleoside transporter

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0631 [new locus tag: SAUSA300_RS03380 ]
  • symbol: SAUSA300_0631
  • product: putative nucleoside transporter
  • replicon: chromosome
  • strand: +
  • coordinates: 705262..706491
  • length: 1230
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTTTTTATTAATCAACATTATTGGTCTAATTGTATTTCTTGGTATTGCGGTATTATTT
    TCAAGAGATCGCAAAAATATCCAATGGCAATCAATTGGGATCTTAGTTGTTTTAAACCTG
    TTTTTAGCATGGTTCTTTATTTATTTTGATTGGGGTCAAAAAGCAGTAAGAGGAGCAGCC
    AATGGTATCGCTTGGGTAGTTCAGTCAGCGCATGCTGGTACAGGTTTTGCATTTGCAAGT
    TTGACAAATGTTAAAATGATGGATATGGCTGTTGCAGCCTTATTCCCAATATTATTAATA
    GTGCCATTATTTGATATCTTAATGTACTTTAATATTTTACCGAAAATTATTGGAGGTATT
    GGTTGGTTACTAGCTAAAGTAACAAGACAACCTAAATTCGAGTCATTCTTTGGGATAGAA
    ATGATGTTCTTAGGAAATACTGAAGCATTAGCCGTATCAAGTGAGCAACTAAAACGTATG
    AATGAAATGCGTGTATTAACAATCGCAATGATGTCAATGAGCTCTGTATCGGGAGCTATT
    GTAGGTGCGTATGTACAAATGGTACCAGGAGAACTGGTACTAACGGCAATTCCACTAAAT
    ATCGTTAACGCGATTATTGTGTCATGCTTGTTGAATCCAGTAAGTGTTGAAGAGAAAGAA
    GATATTATTTACAGTCTTAAAAACAATGAAGTTGAACGTCAACCATTCTTCTCATTCCTT
    GGAGATTCTGTATTAGCAGCAGGTAAATTAGTATTAATCATCATCGCATTTGTTATTAGT
    TTTGTAGCGTTAGCTGATCTATTTGATCGTTTTATCAATTTGATTACAGGATTGATAGCA
    GGATGGATAGGCATAAAAGGTAGTTTCGGTTTAAACCAAATTTTAGGTGTGTTTATGTAT
    CCATTTGCGCTATTACTCGGTTTACCTTATGATGAAGCGTGGTTGGTAGCACAACAAATG
    GCTAAGAAAATTGTTACAAATGAATTTGTTGTTATGGGTGAAATTTCTAAAGATATTGCA
    TCTTATACACCACACCATCGTGCGGTTATTACAACATTCTTAATTTCATTTGCAAACTTC
    TCAACGATTGGTATGATTATCGGTACATTGAAAGGCATTGTTGATAAAAAGACATCAGAC
    TTTGTATCTAAATATGTACCTATGATGCTATTATCAGGTATCCTAGTTTCATTATTAACA
    GCAGCTTTCGTTGGTTTATTTGCATGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1230

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0631 [new locus tag: SAUSA300_RS03380 ]
  • symbol: SAUSA300_0631
  • description: putative nucleoside transporter
  • length: 409
  • theoretical pI: 8.38842
  • theoretical MW: 45015.6
  • GRAVY: 0.900244

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines nucleoside transporter, NupC family (TIGR00804; HMM-score: 322.1)
  • TheSEED  :
    • nucleoside transport protein
  • PFAM:
    no clan defined Nucleos_tra2_C; Na+ dependent nucleoside transporter C-terminus (PF07662; HMM-score: 157.5)
    and 2 more
    Nucleos_tra2_N; Na+ dependent nucleoside transporter N-terminus (PF01773; HMM-score: 65.9)
    DUF3290; Protein of unknown function (DUF3290) (PF11694; HMM-score: 13.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 9
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001584
    • TAT(Tat/SPI): 0.000165
    • LIPO(Sec/SPII): 0.056472
  • predicted transmembrane helices (TMHMM): 10

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAWFFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVPLFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRMNEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEEKEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLITGLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGEISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLSGILVSLLTAAFVGLFAW

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]