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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0636 [new locus tag: SAUSA300_RS03410 ]
  • pan locus tag?: SAUPAN002528000
  • symbol: SAUSA300_0636
  • pan gene symbol?: dhaK
  • synonym:
  • product: dihydroxyacetone kinase subunit DhaK

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0636 [new locus tag: SAUSA300_RS03410 ]
  • symbol: SAUSA300_0636
  • product: dihydroxyacetone kinase subunit DhaK
  • replicon: chromosome
  • strand: +
  • coordinates: 711215..712183
  • length: 969
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTG
    TTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAG
    AAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGAACCTGCGCATGCC
    GGTTTTGTTGCAGAAGGTATGCTAGATGCAGCGGTTTGTGGCGAAGTATTTACATCACCT
    ACACCTGATAAAATATTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTA
    GTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAA
    ATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAA
    GTACAACGTCGTGGTGTTGCAGGAACAGTGTTTGTTCATAAGCTTGCCGGTTATCTTGCT
    GAAAAAGGTTATTCATTAACAGAGATAAAATCGCGTGTAGAAGCGTTGTTACCTGAAATT
    AAAAGTATTGGTATGGCAATTGAGCCACCGCTTGTTCCAACTACTGGAAAATATGGCTTT
    GATATTGAAGACGACAAAATGGAAATCGGTATTGGTATACATGGTGAAAAAGGTATTCAT
    AGGGAAGAAGTAAAGGATATTGATCATATTGTTGGAACATTGTTAGACGAATTGTATAAA
    GAAGTTACTGCCAATGATGTCATATTAATGGTAAATGGTATGGGTGGTACGCCGTTATCT
    GAATTAAATATCGTAACTAAATATATTCAACAAAATTTAGCTGCAAGAACGGTTAATGTT
    GCTAAATGGTTTGTTGGTGATTATATGACATCTTTAGACATGCAAGGTTTTTCTATAACT
    ATCGTGCCTAATAAACCAGAATATTTGGAAGCATTTTTAGCACCAACAACAAGTCAATAC
    TTTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    969

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0636 [new locus tag: SAUSA300_RS03410 ]
  • symbol: SAUSA300_0636
  • description: dihydroxyacetone kinase subunit DhaK
  • length: 322
  • theoretical pI: 4.70202
  • theoretical MW: 35018.3
  • GRAVY: 0.0608696

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.-?  ExPASy
  • TIGRFAM:
    dihydroxyacetone kinase, DhaK subunit (TIGR02363; EC 2.7.1.-; HMM-score: 479.3)
    and 3 more
    dihydroxyacetone kinase (TIGR02361; EC 2.7.1.29; HMM-score: 311.7)
    probable dihydroxyacetone kinase DhaK1b subunit (TIGR02362; HMM-score: 263)
    dihydroxyacetone kinase, phosphotransfer subunit (TIGR02364; HMM-score: 14.2)
  • TheSEED  :
    • Phosphoenolpyruvate-dihydroxyacetone phosphotransferase (EC 2.7.1.121), dihydroxyacetone binding subunit DhaK
    Carbohydrates Central carbohydrate metabolism Dihydroxyacetone kinases  Phosphoenolpyruvate-dihydroxyacetone phosphotransferase (EC 2.7.1.121), dihydroxyacetone binding subunit DhaK
  • PFAM:
    EDD (CL0245) Dak1; Dak1 domain (PF02733; HMM-score: 384)
    and 2 more
    EIIA-man; PTS system fructose IIA component (PF03610; HMM-score: 14.6)
    no clan defined DUF3139; Protein of unknown function (DUF3139) (PF11337; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011421
    • TAT(Tat/SPI): 0.00143
    • LIPO(Sec/SPII): 0.000659
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHGVAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVVKNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLAEKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKGIHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAARTVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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