NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0655 [new locus tag: SAUSA300_RS03510 ]
• pan locus tag^?: SAUPAN002549000
• symbol: SAUSA300_0655
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0655 [new locus tag: SAUSA300_RS03510 ]
• symbol: SAUSA300_0655
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 731805..732521
• length: 717
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912854 NCBI
• RefSeq: YP_493358 NCBI
• BioCyc: GH3C-650 BioCyc
• MicrobesOnline: 1292170 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGT
  AGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAAT
  CCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCG
  AATCATATTATTGAAAAAGCAATAGATAAAGCTAAGGGTGCTGGAGACGAAAACTTTGAT
  CACCTAAGATATGAAGGATTTGGCCCAAGCGGATCAATGCTAATTGTTGATGCGTTAACA
  AATAATGTAAATCGTACTGCCTCTGATGTGCGAGCTGCTTTTGGTAAAAACGGCGGTAAT
  ATGGGTGTATCTGGATCAGTTGCTTATATGTTTGATCATGTGGCAACATTTGGTATTGAA
  GGAAAGTCTGTTGACGAAATACTTGAAACATTAATGGAACAAGATGTAGATGTAAATGAT
  GTGATTGACGATAATGGATTGACAATAGTCTATGCTGAACCAGATCAATTTGCAGTCGTT
  CAAGATGCGCTTCGTGCAGCAGGTGTTGAAGAATTTAAAGTTGCTGAATTTGAAATGTTA
  CCTCAAACAGATATTGAACTTTCTGAAGCGGACCAAGTAACATTTGAAAAATTAATCGAT
  GCATTAGAAGATTTAGAAGATGTACAAAACGTATTCCATAATGTGGATTTGAAATAA
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  717
  

⊟Protein

⊟General

• locus tag: SAUSA300_0655 [new locus tag: SAUSA300_RS03510 ]
• symbol: SAUSA300_0655
• description: hypothetical protein
• length: 238
• theoretical pI: 4.37556
• theoretical MW: 26320.2
• GRAVY: -0.413445

⊟Function

• TIGRFAM:
  Signal transductionRegulatory functionsDNA interactionsDNA-binding regulatory protein, YebC/PmpR family (TIGR01033; HMM-score: 227.2)
  and 1 more
  Genetic information processingProtein fateProtein and peptide secretion and traffickingtype VII secretion protein EccB (TIGR03919; HMM-score: 11)
• TheSEED:  
  Transcriptional regulatory protein YeeN
• PFAM:
  no clan definedTranscrip_reg; Transcriptional regulator (PF01709; HMM-score: 264.8)
  and 2 more
  GatB_YqeY (CL0279) GatB_Yqey; GatB domain (PF02637; HMM-score: 14.1)
  Ank (CL0465) Ank_4; Ankyrin repeats (many copies) (PF13637; HMM-score: 12.4)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.01061
  • TAT(Tat/SPI): 0.000749
  • LIPO(Sec/SPII): 0.002688
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160409 NCBI
• RefSeq: YP_493358 NCBI
• UniProt: Q2FIX0 UniProt

⊟Protein sequence

• MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESNQALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNNVNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVNDVIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKLIDALEDLEDVQNVFHNVDLK

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0655 > SAUSA300_0656

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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