PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0686 [new locus tag: SAUSA300_RS03685 ]
• pan locus tag^?: SAUPAN002586000
• symbol: nagA
• pan gene symbol^?: nagA
• synonym:
• product: N-acetylglucosamine-6-phosphate deacetylase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0686 [new locus tag: SAUSA300_RS03685 ]
• symbol: nagA
• product: N-acetylglucosamine-6-phosphate deacetylase
• replicon: chromosome
• strand: +
• coordinates: 761187..762368
• length: 1182
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915295 NCBI
• RefSeq: YP_493389 NCBI
• BioCyc: see SAUSA300_RS03685
• MicrobesOnline: 1292201 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  GTGTCAGAATTAATTATATATAACGGCAAAGTTTATACTGAAGATGGCAAAATCGATAAT
  GGTTACATTCATGTGAAAGATGGACAGATTGTTGCAATTGGAGAAGTGGATGATAAAGCA
  GCAATTGATAATGATACGACAAATAAAATTCAAGTGATTGATGCTAAAGGTCATCATGTA
  TTACCAGGTTTTATTGATATACATATTCATGGTGGTTATGGTCAAGATGCAATGGATGGG
  TCATACGATGGCTTAAAATATCTATCCGAAAATTTGTTGTCTGAAGGGACGACATCATAC
  TTGGCCACTACAATGACGCAATCGACTGATAAAATAGATAATGCACTTACAAATATTGCT
  AAATATGAAGCGGAGCAAGATGTTCACAATGCAGCGGAAATTGTAGGTATACATTTAGAA
  GGACCATTTATATCTGAAAATAAAGTTGGTGCTCAACATCCGCAATACGTTGTACGCCCA
  TTTATCGATAAAATTAAACATTTTCAAGAGACTGCTAACGGATTAATAAAGATTATGACG
  TTTGCACCTGAAGTTGAAGGTGCAAAAGAAGCGCTTGAAACGTATAAAGATGACATTATT
  TTTTCAATTGGTCATACAGTAGCAACATACGAAGAAGCAGTTGAAGCTGTTGAGCGAGGA
  GCTAAACATGTCACGCATTTATATAATGCAGCGACGCCATTCCAACATAGAGAACCAGGT
  GTTTTTGGAGCAGCATGGTTGAATGATGCTCTACATACCGAAATGATTGTTGATGGCACT
  CATTCTCATCCGGCATCGGTTGCAATTGCTTACCGTATGAAAGGTAATGAACGTTTTTAT
  TTAATTACCGATGCAATGCGTGCAAAAGGTATGCCTGAAGGAGAATATGATTTGGGTGGA
  CAAAAAGTAACTGTTCAATCGCAACAAGCACGTCTTGCAAATGGTGCGCTTGCTGGTAGT
  ATTTTAAAAATGAATCATGGGTTACGTAACTTAATATCATTTACAGGTGATACATTAGAT
  CATTTATGGCGAGTAACAAGTTTAAATCAAGCCATTGCATTAGGTATCGATGATAGAAAA
  GGTAGTATTAAAGTAAATAAGGATGCAGATCTTGTTATTCTAGATGATGATATGAATGTA
  AAATCTACAATAAAACAAGGCAAGGTTCACACATTTAGCTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0686 [new locus tag: SAUSA300_RS03685 ]
• symbol: NagA
• description: N-acetylglucosamine-6-phosphate deacetylase
• length: 393
• theoretical pI: 5.49488
• theoretical MW: 43115.2
• GRAVY: -0.302799

⊟Function

• reaction:
  EC 3.5.1.25?  ExPASy
  N-acetylglucosamine-6-phosphate deacetylase N-acetyl-D-glucosamine 6-phosphate + H₂O = D-glucosamine 6-phosphate + acetate
• TIGRFAM:
  Metabolism Central intermediary metabolism Amino sugars N-acetylglucosamine-6-phosphate deacetylase (TIGR00221; EC 3.5.1.25; HMM-score: 378.9)
  and 12 more
  allantoinase (TIGR03178; EC 3.5.2.5; HMM-score: 45.8)
  Metabolism Energy metabolism Amino acids and amines imidazolonepropionase (TIGR01224; EC 3.5.2.7; HMM-score: 44.2)
  dihydropyrimidinase (TIGR02033; EC 3.5.2.2; HMM-score: 42.9)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other guanine deaminase (TIGR02967; EC 3.5.4.3; HMM-score: 41.1)
  Metabolism Energy metabolism Other phosphonate metabolism protein PhnM (TIGR02318; HMM-score: 39.6)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides beta-aspartyl peptidase (TIGR01975; EC 3.4.19.5; HMM-score: 36.3)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine deaminase (TIGR01178; EC 3.5.4.2; HMM-score: 36.2)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis dihydroorotase, multifunctional complex type (TIGR00857; EC 3.5.2.3; HMM-score: 33.8)
  formylmethanofuran dehydrogenase subunit A (TIGR03121; EC 1.2.99.5; HMM-score: 26.4)
  Metabolism Central intermediary metabolism Nitrogen metabolism urease, alpha subunit (TIGR01792; EC 3.5.1.5; HMM-score: 19.7)
  putative selenium metabolism protein SsnA (TIGR03314; HMM-score: 17.8)
  Metabolism Energy metabolism Amino acids and amines formiminoglutamate deiminase (TIGR02022; EC 3.5.3.13; HMM-score: 13.8)
• TheSEED  :
  • N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25)
  Carbohydrates Aminosugars Chitin and N-acetylglucosamine utilization  N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25)
  and 2 more
  Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25)
  Cell Wall and Capsule Cell Wall and Capsule - no subcategory UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis  N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25)
• PFAM:
  Amidohydrolase (CL0034) Amidohydro_1; Amidohydrolase family (PF01979; HMM-score: 96.5)
  and 1 more
  Amidohydro_3; Amidohydrolase family (PF07969; HMM-score: 56.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: a divalent metal cation
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004913
  • TAT(Tat/SPI): 0.000179
  • LIPO(Sec/SPII): 0.000803
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161324 NCBI
• RefSeq: YP_493389 NCBI
• UniProt: A0A0H2XGG9 UniProt

⊟Protein sequence

• MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEVDDKAAIDNDTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLATTMTQSTDKIDNALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRPFIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVERGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGNERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISFTGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTFS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications