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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0687 [new locus tag: SAUSA300_RS03690 ]
  • pan locus tag?: SAUPAN002587000
  • symbol: SAUSA300_0687
  • pan gene symbol?: mpfA
  • synonym:
  • product: putative hemolysin

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0687 [new locus tag: SAUSA300_RS03690 ]
  • symbol: SAUSA300_0687
  • product: putative hemolysin
  • replicon: chromosome
  • strand: +
  • coordinates: 762589..763938
  • length: 1350
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    TTGGAAACTTCGACCATAATTAGTTTGATTATATTTATTCTATTAATTGCATTAACCACT
    GTATTTGTTGGTTCAGAATTTGCATTAGTAAAAATTAGAGCAACAAGAATTGAACAGCTA
    GCAGATGAAGGAAATAAACCTGCTAAAATAGTAAAAAAGATGATTGCTAATCTAGATTAT
    TATCTTTCTGCTTGTCAGTTAGGTATAACAGTAACATCTTTAGGGTTAGGTTGGCTTGGT
    GAACCAACGTTTGAAAAGCTATTACACCCAATATTTGAAGCAATCAATTTACCAACTGCA
    TTAACGACGACGATTTCGTTTGCAGTGTCATTTATAATCGTTACGTATTTGCATGTAGTA
    CTTGGTGAATTAGCGCCTAAATCTATAGCTATTCAACATACTGAAAAGCTTGCTTTAGTA
    TATGCAAGACCATTGTTCTATTTCGGTAACATTATGAAACCATTGATTTGGCTGATGAAT
    GGTTCTGCACGTGTTATTATTAGAATGTTTGGTGTAAATCCTGATGCCCAAACTGATGCA
    ATGTCAGAAGAAGAAATCAAAATTATTATTAACAATAGTTATAATGGTGGAGAAATCAAC
    CAAACTGAATTGGCATATATGCAAAATATCTTTTCATTCGATGAAAGACATGCAAAAGAT
    ATAATGGTACCTAGAACTCAAATGATTACACTAAATGAACCTTTTAATGTAGACGAATTA
    CTAGAAACAATAAAAGAACATCAATTTACGCGTTATCCAATTACTGATGATGGTGATAAA
    GACCACATTAAAGGATTTATTAACGTCAAAGAATTTTTAACTGAATACGCTTCTGGAAAA
    ACGATTAAAATAGCAAACTATATACATGAGTTGCCAATGATTTCAGAGACAACACGTATC
    AGTGATGCATTAATTAGAATGCAACGTGAACATGTACATATGAGTCTTATTATAGATGAA
    TATGGTGGAACGGCAGGTATTTTAACGATGGAAGATATTTTAGAAGAAATCGTTGGAGAA
    ATTCGTGATGAATTTGATGATGATGAAGTGAATGATATCGTTAAAATTGATAATAAGACA
    TTCCAAGTAAATGGCAGAGTACTATTGGATGATTTAACTGAAGAGTTCGGTATAGAATTT
    GATGACTCTGAGGATATTGATACGATAGGTGGATGGTTACAATCTCGTAATACCAATTTA
    CAAAAAGATGATTACGTGGATACAACTTATGATCGCTGGGTTGTTTCAGAAATCGATAAC
    CACCAAATTATTTGGGTGATATTAAACTATGAATTTAATGAAGCGAGACCTACTATCGGA
    CAGTCTGATGAAGATGAAAAATCAGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1350

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0687 [new locus tag: SAUSA300_RS03690 ]
  • symbol: SAUSA300_0687
  • description: putative hemolysin
  • length: 449
  • theoretical pI: 4.25784
  • theoretical MW: 51150
  • GRAVY: -0.0665924

Function[edit | edit source]

  • TIGRFAM:
    gliding motility-associated protein GldE (TIGR03520; HMM-score: 172.3)
    and 2 more
    Metabolism Energy metabolism Sugars sugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 24.8)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis inosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 23.6)
  • TheSEED  :
    • Hemolysins and related proteins containing CBS domains
  • PFAM:
    no clan defined DUF21; Domain of unknown function DUF21 (PF01595; HMM-score: 169.3)
    and 3 more
    CBS; CBS domain (PF00571; HMM-score: 52.8)
    CorC_HlyC; Transporter associated domain (PF03471; HMM-score: 51.3)
    MGS; MGS-like domain (PF02142; HMM-score: 13.9)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • LocateP: Multi-transmembrane(Lipid modified N-termini)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: YLSACQL
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.311499
    • TAT(Tat/SPI): 0.003521
    • LIPO(Sec/SPII): 0.039307
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEGNKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALTTTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMNGSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHAKDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEYASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDILEEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGWLQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

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Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]