PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0687 [new locus tag: SAUSA300_RS03690 ]
• pan locus tag^?: SAUPAN002587000
• symbol: SAUSA300_0687
• pan gene symbol^?: mpfA
• synonym:
• product: putative hemolysin

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0687 [new locus tag: SAUSA300_RS03690 ]
• symbol: SAUSA300_0687
• product: putative hemolysin
• replicon: chromosome
• strand: +
• coordinates: 762589..763938
• length: 1350
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914045 NCBI
• RefSeq: YP_493390 NCBI
• BioCyc: see SAUSA300_RS03690
• MicrobesOnline: 1292202 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  TTGGAAACTTCGACCATAATTAGTTTGATTATATTTATTCTATTAATTGCATTAACCACT
  GTATTTGTTGGTTCAGAATTTGCATTAGTAAAAATTAGAGCAACAAGAATTGAACAGCTA
  GCAGATGAAGGAAATAAACCTGCTAAAATAGTAAAAAAGATGATTGCTAATCTAGATTAT
  TATCTTTCTGCTTGTCAGTTAGGTATAACAGTAACATCTTTAGGGTTAGGTTGGCTTGGT
  GAACCAACGTTTGAAAAGCTATTACACCCAATATTTGAAGCAATCAATTTACCAACTGCA
  TTAACGACGACGATTTCGTTTGCAGTGTCATTTATAATCGTTACGTATTTGCATGTAGTA
  CTTGGTGAATTAGCGCCTAAATCTATAGCTATTCAACATACTGAAAAGCTTGCTTTAGTA
  TATGCAAGACCATTGTTCTATTTCGGTAACATTATGAAACCATTGATTTGGCTGATGAAT
  GGTTCTGCACGTGTTATTATTAGAATGTTTGGTGTAAATCCTGATGCCCAAACTGATGCA
  ATGTCAGAAGAAGAAATCAAAATTATTATTAACAATAGTTATAATGGTGGAGAAATCAAC
  CAAACTGAATTGGCATATATGCAAAATATCTTTTCATTCGATGAAAGACATGCAAAAGAT
  ATAATGGTACCTAGAACTCAAATGATTACACTAAATGAACCTTTTAATGTAGACGAATTA
  CTAGAAACAATAAAAGAACATCAATTTACGCGTTATCCAATTACTGATGATGGTGATAAA
  GACCACATTAAAGGATTTATTAACGTCAAAGAATTTTTAACTGAATACGCTTCTGGAAAA
  ACGATTAAAATAGCAAACTATATACATGAGTTGCCAATGATTTCAGAGACAACACGTATC
  AGTGATGCATTAATTAGAATGCAACGTGAACATGTACATATGAGTCTTATTATAGATGAA
  TATGGTGGAACGGCAGGTATTTTAACGATGGAAGATATTTTAGAAGAAATCGTTGGAGAA
  ATTCGTGATGAATTTGATGATGATGAAGTGAATGATATCGTTAAAATTGATAATAAGACA
  TTCCAAGTAAATGGCAGAGTACTATTGGATGATTTAACTGAAGAGTTCGGTATAGAATTT
  GATGACTCTGAGGATATTGATACGATAGGTGGATGGTTACAATCTCGTAATACCAATTTA
  CAAAAAGATGATTACGTGGATACAACTTATGATCGCTGGGTTGTTTCAGAAATCGATAAC
  CACCAAATTATTTGGGTGATATTAAACTATGAATTTAATGAAGCGAGACCTACTATCGGA
  CAGTCTGATGAAGATGAAAAATCAGAATAG
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⊟Protein

⊟General

• locus tag: SAUSA300_0687 [new locus tag: SAUSA300_RS03690 ]
• symbol: SAUSA300_0687
• description: putative hemolysin
• length: 449
• theoretical pI: 4.25784
• theoretical MW: 51150
• GRAVY: -0.0665924

⊟Function

• TIGRFAM:
  gliding motility-associated protein GldE (TIGR03520; HMM-score: 172.3)
  and 2 more
  Metabolism Energy metabolism Sugars sugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 24.8)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis inosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 23.6)
• TheSEED  :
  • Hemolysins and related proteins containing CBS domains
• PFAM:
  no clan defined DUF21; Domain of unknown function DUF21 (PF01595; HMM-score: 169.3)
  and 3 more
  CBS; CBS domain (PF00571; HMM-score: 52.8)
  CorC_HlyC; Transporter associated domain (PF03471; HMM-score: 51.3)
  MGS; MGS-like domain (PF02142; HMM-score: 13.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 3
• LocateP: Multi-transmembrane(Lipid modified N-termini)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: YLSACQL
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.311499
  • TAT(Tat/SPI): 0.003521
  • LIPO(Sec/SPII): 0.039307
• predicted transmembrane helices (TMHMM): 4

⊟Accession numbers

• GI: 87161802 NCBI
• RefSeq: YP_493390 NCBI
• UniProt: A0A0H2XI09 UniProt

⊟Protein sequence

• METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEGNKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALTTTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMNGSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHAKDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEYASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDILEEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGWLQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications