From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0707 [new locus tag: SAUSA300_RS03800 ]
  • pan locus tag?: SAUPAN002610000
  • symbol: SAUSA300_0707
  • pan gene symbol?: opuBB
  • synonym:
  • product: osmoprotectant ABC transporter permease

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0707 [new locus tag: SAUSA300_RS03800 ]
  • symbol: SAUSA300_0707
  • product: osmoprotectant ABC transporter permease
  • replicon: chromosome
  • strand: +
  • coordinates: 784224..785738
  • length: 1515
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGACTAACTTTTTCGACATATTGAGTGAACGTAAGGGGCAACTCTTTTCGACAATGATA
    GAACATATTCAAATATCATTTATCGCATTATTGATTGCAACTGCTATTGCGGTACCATTA
    GGTATTTTATTAACGAAGACTAAAACGATATCTGAAATCGTAATGAATATTGCGGCAATT
    CTTCAAACCATACCATCGTTGGCATTATTAGGTTTAATGATTCCTTTATTTGGTATCGGT
    CGTGTGCCAGCAATTATTGCACTTGTAGTGTATGCGTTGTTACCAATTTTAAGGAATACG
    TATACTGGAATTAAAGAAGTTGATCCATCACTCATTGAAGCGGCTAAAGGTATAGGTATG
    AAACCATTTAGACGTTTAACTAAAGTCGAACTTCCGATAGCAATGCCTGTTATAATGGCT
    GGTGTAAGAACGGCTATGGTATTAATTATAGGTACAGCAACACTAGCAGCATTAATTGGT
    GCAGGCGGACTAGGAGATTTAATTTTATTAGGTATAGACCGTAACAATGCATCGTTGATA
    TTATTAGGTGCAATTCCAGCAGCCTTATTGGCAATTATATTTGATTTAATTTTAAGATTT
    ATGGCTAAATTATCTTATAAAAAGTTATTGATGACGTTAGGTGTTATAGTGATGATTATT
    ATACTGGCTATCGCTATTCCTATGTTTGCACAAAAAGGTGATAAAATTACGTTAGCTGGA
    AAGCTTGGCTCCGAGCCATCGATTATTACAAATATGTATAAAATTTTAATAGAAGAAGAG
    ACCAAAAATACTGTAGAAGTGAAAGATGGTATGGGCAAAACAGCATTTTTATTTAATGCT
    TTAAAATCTGACGATATAGATGGGTATTTAGAATTTACTGGAACAGTTTTAGGTGAATTA
    ACAAAAGAACCATTGAAGTCAAAAGAAGAGAAAAAAGTTTATGAACAAGCTAAGCAAAGT
    CTTGAAAAGAAATATCAAATGACTATGTTAAAACCAATGAAGTATAACAATACGTATGCT
    TTAGCTGTAAAACGTGATTTTGCTAAACAACATAATATACGTACAATTGGTGATTTAAAT
    AAGGTTAAAGATCAACTTAAACCAGGATTTACATTGGAATTTAATGATCGTCCAGATGGT
    TACAAAGCTGTTCAAAAGGCTTATAATTTAAATTTAGATAACATACGTACAATGGAACCT
    AAGTTGAGATATCAAGCGATCAATAAAGGTAATATTAATTTAATAGATGCATATTCAACT
    GACGCTGAATTAAAACAATATGATATGGTTGTGTTAAAAGATGATAAGCACGTATTTCCA
    CCATATCAAGGAGCACCATTATTTAAAGAAAGCTTTTTAAAGAAACATCCAGAAATTAAG
    AAACCGTTAAACAAACTAGAAAACAAAATATCTGATGAAGATATGCAAATGATGAACTAT
    AAAGTAACAGTTAAAAATGAAGACCCATATACAGTTGCGAAAGATTATTTAAAAGCAAAA
    GGGTTAATCAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1515

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0707 [new locus tag: SAUSA300_RS03800 ]
  • symbol: SAUSA300_0707
  • description: osmoprotectant ABC transporter permease
  • length: 504
  • theoretical pI: 9.9494
  • theoretical MW: 56057.4
  • GRAVY: 0.150794

Function[edit | edit source]

  • reaction:
    EC 3.6.3.32?  ExPASy
    Quaternary-amine-transporting ATPase ATP + H2O + quaternary amine(Out) = ADP + phosphate + quaternary amine(In)
  • TIGRFAM:
    choline ABC transporter, permease protein (TIGR03416; HMM-score: 113.1)
    and 8 more
    Metabolism Transport and binding proteins Anions nitrate ABC transporter, permease protein (TIGR01183; HMM-score: 47)
    Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 45.8)
    2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 27.5)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 21.3)
    2-aminoethylphosphonate ABC transport system, membrane component PhnV (TIGR03255; HMM-score: 19.6)
    NifC-like ABC-type porter (TIGR01581; HMM-score: 19)
    Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 18.2)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 8.4)
  • TheSEED:  
    Stress Response Osmotic stress Choline and Betaine Uptake and Betaine Biosynthesis  Glycine betaine ABC transport system, permease protein OpuAB
    and 1 more
    L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1)
  • PFAM:
    PBP (CL0177) OpuAC; Substrate binding domain of ABC-type glycine betaine transport system (PF04069; HMM-score: 210.3)
    and 2 more
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 93.7)
    CYTH (CL0273) Med18; Med18 protein (PF09637; HMM-score: 13.9)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007348
    • TAT(Tat/SPI): 0.000625
    • LIPO(Sec/SPII): 0.00171
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILLTKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYTGIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIGAGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVMIIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAFLFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMKYNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNLDNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKESFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]