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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0736 [new locus tag: SAUSA300_RS03960 ]
  • pan locus tag?: SAUPAN002661000
  • symbol: yfiA
  • pan gene symbol?: saHPF
  • synonym:
  • product: ribosomal subunit interface protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0736 [new locus tag: SAUSA300_RS03960 ]
  • symbol: yfiA
  • product: ribosomal subunit interface protein
  • replicon: chromosome
  • strand: +
  • coordinates: 816429..817001
  • length: 573
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGATTAGATTTGAAATTCATGGAGATAACCTCACTATCACAGATGCTATTCGCAACTAT
    ATTGAGGAAAAAATTGGTAAGTTGGAACGTTATTTTAATGACGTACCAAATGCAGTGGCG
    CATGTTAAAGTTAAAACTTATTCAAATTCAGCTACTAAAATTGAAGTAACAATTCCATTG
    AAAAATGTTACGTTAAGAGCTGAAGAGCGAAACGATGATTTATACGCAGGTATTGATTTA
    ATTAATAATAAACTTGAAAGACAAGTTCGAAAATATAAAACACGTATTAATCGTAAGAGC
    CGTGATCGAGGAGATCAAGAAGTGTTTGTTGCCGAATTACAAGAAATGCAAGAAACACAA
    GTTGATAATGACGCTTACGATGATAACGAGATAGAAATTATTCGTTCAAAAGAATTCAGC
    TTAAAACCAATGGATTCAGAAGAAGCGGTATTACAAATGAATCTATTAGGTCATGACTTC
    TTTGTATTCACAGACAGAGAAACTGATGGAACAAGTATCGTTTACCGCCGTAAAGACGGT
    AAATATGGCTTGATTCAAACTAGTGAACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    573

⊟Protein[edit | edit source]

Protein Data Bank: 5ND8
Protein Data Bank: 5ND9
Protein Data Bank: 5NKO

⊟General[edit | edit source]

  • locus tag: SAUSA300_0736 [new locus tag: SAUSA300_RS03960 ]
  • symbol: YfiA
  • description: ribosomal subunit interface protein
  • length: 190
  • theoretical pI: 4.91383
  • theoretical MW: 22212.7
  • GRAVY: -0.763158

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis Translation factors ribosomal subunit interface protein (TIGR00741; HMM-score: 101.8)
  • TheSEED  :
    • Ribosome hibernation promoting factor Hpf
    Protein Metabolism Protein biosynthesis Ribosome activity modulation  Ribosomal subunit interface protein
  • PFAM:
    no clan defined Ribosom_S30AE_C; Sigma 54 modulation/S30EA ribosomal protein C terminus (PF16321; HMM-score: 97)
    Ribosomal_S30AE; Sigma 54 modulation protein / S30EA ribosomal protein (PF02482; HMM-score: 92.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002633
    • TAT(Tat/SPI): 0.000342
    • LIPO(Sec/SPII): 0.00041
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKVKTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRDRGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDFFVFTDRETDGTSIVYRRKDGKYGLIQTSEQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism;  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Arnab Basu, Kathryn E Shields, Christopher S Eickhoff, Daniel F Hoft, M N Yap
    Thermal and Nutritional Regulation of Ribosome Hibernation in Staphylococcus aureus.
    J Bacteriol: 2018, 200(24);
    [PubMed:30297357] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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