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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0780 [new locus tag: SAUSA300_RS04205 ]
  • pan locus tag?: SAUPAN002805000
  • symbol: SAUSA300_0780
  • pan gene symbol?: lnsA
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0780 [new locus tag: SAUSA300_RS04205 ]
  • symbol: SAUSA300_0780
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 868606..869175
  • length: 570
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    GTGAACTATATTAAACGAACGATAATTTTACTTATATTATTCGTTGTCGTATCCCCAATA
    AACTCACCAAAAACAATTGCAGATAATAAGTATTCAGAAATTCAAGATGACAAATTCCAA
    TTACAACCTGGTGATATTATTGTTACAAAAGGCCCTGTCATGTGGGGATTTTTTGGTCAT
    TGTAGTATCGCGATTGATGATAAAACGATTTTACAAATTGAGGGGCCTGGCGACAAACCA
    ACTACACAATCCTTCGAATCTTTTAAATATAATTATGCAAGTGGCAAAAATGATTGGATG
    AAAGTTTATCGTTGCAGCTATCCTGGTGCAGGTAAAAAAGCGGCTGAATGGGTTAAGAAA
    AACTATGAGAATACAAATCATCGTTATCTAGTTACATTAAATTTGAACAGCAAAAAATTT
    ACGTATTGTACTAAAATTATCTACCAAGCTTATAAATTTGGTGTAAGTGAAAAATCAGTT
    AAAAGTTATGGACTACATATTATTTCACCCTATGCAATTAAAGATAATTTTATAGATCCA
    TACAAACTAAGGCTTGTTAAAGCTTATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    570

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0780 [new locus tag: SAUSA300_RS04205 ]
  • symbol: SAUSA300_0780
  • description: hypothetical protein
  • length: 189
  • theoretical pI: 9.80772
  • theoretical MW: 21755
  • GRAVY: -0.333862

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • FIG01107922: hypothetical protein
  • PFAM:
    Peptidase_CA (CL0125) Peptidase_C92; Permuted papain-like amidase enzyme, YaeF/YiiX, C92 family (PF05708; HMM-score: 22.1)
    and 2 more
    RING (CL0229) zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein (PF02148; HMM-score: 13.2)
    Cystatin (CL0121) DUF3889; Protein of unknown function (DUF3889) (PF13028; HMM-score: 12.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 7
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.931027
    • TAT(Tat/SPI): 0.007444
    • LIPO(Sec/SPII): 0.018888
    • Cleavage Site: CS pos: 27-28. TIA-DN. Pr: 0.8438
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPGDIIVTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKVYRCSYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSVKSYGLHIISPYAIKDNFIDPYKLRLVKAY

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]