PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0796 [new locus tag: SAUSA300_RS04300 ]
• pan locus tag^?: SAUPAN002838000
• symbol: SAUSA300_0796
• pan gene symbol^?: metN1
• synonym:
• product: ABC transporter ATP-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0796 [new locus tag: SAUSA300_RS04300 ]
• symbol: SAUSA300_0796
• product: ABC transporter ATP-binding protein
• replicon: chromosome
• strand: +
• coordinates: 879301..880326
• length: 1026
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914789 NCBI
• RefSeq: YP_493496 NCBI
• BioCyc: see SAUSA300_RS04300
• MicrobesOnline: 1292311 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  
  GTGATTGAATTAAAAGAAGTTGTTAAAGAATATCGGACTAAAAATAAAGAAGTCCTTGCT
  GTAGATCACGTTAATTTATCGATTCGAGCAGGATCGATTTATGGCGTCATTGGTTTTTCT
  GGAGCAGGAAAAAGTACTTTGATTCGAATGTTTAATCATTTAGAAGCGCCTACATCAGGT
  GAAGTTATTATAGATGGAGACCATATAGGTCAATTGTCCAAAAATGGATTAAGAGCAAAA
  AGACAAAAAGTAAGTATGATCTTCCAACATTTTAATTTGTTATGGTCAAGGACTGTGTTA
  AAAAATATTATGTTTCCGCTTGAAATTGCAGGTGTCCCTAGAAGGAGAGCTAAGCAAAAA
  GCATTAGAACTTGTCGAACTCGTCGGTTTAAAAGGTAGAGAAAAGGCTTATCCATCAGAG
  TTATCAGGTGGACAAAAGCAACGTGTTGGGATTGCACGAGCGTTAGCTAATGATCCAACG
  GTCTTGCTTTGTGATGAGGCAACAAGTGCACTTGATCCGCAAACAACAGATGAAATTTTA
  GATCTACTACTAAAAATTAGAGAACAACAAAATTTAACAATTGTACTAATTACGCATGAA
  ATGCATGTCATTCGTCGTATTTGTGATGAAGTTGCAGTTATGGAAAGTGGTAAAGTGATA
  GAACAAGGACCGGTGACACAGGTTTTTGAAAATCCGCAACACACTGTGACAAAACGATTT
  GTGAAAGACGATTTAAATGATGATTTCGAAACATCTTTAACAGAATTAGAGCCATTAGAA
  AAAGATGCATATATCGTTAGATTAGTTTTCGCTGGTTCAACAACAACCGAGCCTATTGTA
  TCGAGTCTATCAACTGCCTATGATATTAAAATTAATATTTTAGAAGCAAATATTAAAAAT
  ACAAAAAATGGAACAGTCGGCTTTTTAGTTCTGCATATTCCATATATTTCAAGTGTAGAT
  TTCGGAAAATTCGAAAAAGAGTTAATTGAGCGACAAGTTAAAATGGAGGTGTTAAGACAT
  GGGTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1026
  

⊟Protein

⊟General

• locus tag: SAUSA300_0796 [new locus tag: SAUSA300_RS04300 ]
• symbol: SAUSA300_0796
• description: ABC transporter ATP-binding protein
• length: 341
• theoretical pI: 7.32519
• theoretical MW: 38208.9
• GRAVY: -0.134604

⊟Function

• reaction:
  EC 3.6.3.-?  ExPASy
• TIGRFAM:
  D-methionine ABC transporter, ATP-binding protein (TIGR02314; EC 3.6.3.-; HMM-score: 358.4)
  and 72 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 235.7)
  ectoine/hydroxyectoine ABC transporter, ATP-binding protein EhuA (TIGR03005; HMM-score: 211.3)
  Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04521; EC 3.6.3.-; HMM-score: 208)
  Metabolism Transport and binding proteins Anions phosphonate ABC transporter, ATP-binding protein (TIGR02315; EC 3.6.3.28; HMM-score: 198)
  Cellular processes Cellular processes Cell division cell division ATP-binding protein FtsE (TIGR02673; HMM-score: 194)
  Metabolism Transport and binding proteins Amino acids, peptides and amines choline ABC transporter, ATP-binding protein (TIGR03415; HMM-score: 191.7)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking lipoprotein releasing system, ATP-binding protein (TIGR02211; EC 3.6.3.-; HMM-score: 190.3)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, ATP-binding protein (TIGR00972; EC 3.6.3.27; HMM-score: 186.7)
  Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04520; EC 3.6.3.-; HMM-score: 186)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, ATP-binding protein (TIGR00968; EC 3.6.3.25; HMM-score: 183.6)
  Metabolism Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, ATP-binding protein (TIGR03265; HMM-score: 173.7)
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel import ATP-binding protein NikE (TIGR02769; EC 3.6.3.24; HMM-score: 170.1)
  Cellular processes Cellular processes Toxin production and resistance putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 166)
  Metabolism Transport and binding proteins Unknown substrate putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 166)
  Metabolism Transport and binding proteins Other daunorubicin resistance ABC transporter, ATP-binding protein (TIGR01188; HMM-score: 165.6)
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel import ATP-binding protein NikD (TIGR02770; HMM-score: 162.3)
  ABC exporter ATP-binding subunit, DevA family (TIGR02982; HMM-score: 157.4)
  Metabolism Transport and binding proteins Amino acids, peptides and amines polyamine ABC transporter, ATP-binding protein (TIGR01187; EC 3.6.3.31; HMM-score: 157.3)
  Metabolism Transport and binding proteins Anions molybdate ABC transporter, ATP-binding protein (TIGR02142; EC 3.6.3.29; HMM-score: 143.7)
  Metabolism Energy metabolism Methanogenesis methyl coenzyme M reductase system, component A2 (TIGR03269; HMM-score: 143.4)
  Cellular processes Cellular processes Pathogenesis type I secretion system ATPase (TIGR03375; HMM-score: 140.9)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR03375; HMM-score: 140.9)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR01846; HMM-score: 139.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids ABC transporter, ATP-binding subunit, PQQ-dependent alcohol dehydrogenase system (TIGR03864; HMM-score: 137.2)
  Metabolism Transport and binding proteins Other antigen peptide transporter 2 (TIGR00958; HMM-score: 136.9)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides lipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 135.9)
  Metabolism Transport and binding proteins Other lipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 135.9)
  Metabolism Transport and binding proteins Other thiamine ABC transporter, ATP-binding protein (TIGR01277; EC 3.6.3.-; HMM-score: 135.1)
  Cellular processes Cellular processes Biosynthesis of natural products NHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 131.8)
  Metabolism Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 131.8)
  Metabolism Transport and binding proteins Anions nitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 130.6)
  Metabolism Transport and binding proteins Other nitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 130.6)
  Cellular processes Cellular processes Biosynthesis of natural products NHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 130.3)
  Metabolism Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 130.3)
  gliding motility-associated ABC transporter ATP-binding subunit GldA (TIGR03522; HMM-score: 128.5)
  2-aminoethylphosphonate ABC transport system, ATP-binding component PhnT (TIGR03258; HMM-score: 127.8)
  ABC transporter, permease/ATP-binding protein (TIGR02204; HMM-score: 125.7)
  Metabolism Transport and binding proteins Cations and iron carrying compounds cobalt ABC transporter, ATP-binding protein (TIGR01166; HMM-score: 125.6)
  thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 120.2)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides LPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 116.3)
  Metabolism Transport and binding proteins Other LPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 116.3)
  lantibiotic protection ABC transporter, ATP-binding subunit (TIGR03740; HMM-score: 115.7)
  Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtE (TIGR03410; HMM-score: 113.7)
  Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtD (TIGR03411; HMM-score: 110.3)
  Cellular processes Cellular processes Other nodulation ABC transporter NodI (TIGR01288; HMM-score: 107.9)
  Metabolism Transport and binding proteins Other nodulation ABC transporter NodI (TIGR01288; HMM-score: 107.9)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking ABC-type bacteriocin transporter (TIGR01193; HMM-score: 107.4)
  Genetic information processing Protein fate Protein modification and repair ABC-type bacteriocin transporter (TIGR01193; HMM-score: 107.4)
  Metabolism Transport and binding proteins Other ABC-type bacteriocin transporter (TIGR01193; HMM-score: 107.4)
  proposed F420-0 ABC transporter, ATP-binding protein (TIGR03873; HMM-score: 106.5)
  thiol reductant ABC exporter, CydC subunit (TIGR02868; HMM-score: 100.1)
  phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 97.7)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR01842; HMM-score: 96.6)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids glucan exporter ATP-binding protein (TIGR01192; EC 3.6.3.42; HMM-score: 95.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-xylose ABC transporter, ATP-binding protein (TIGR02633; EC 3.6.3.17; HMM-score: 92.3)
  Metabolism Transport and binding proteins Other rim ABC transporter (TIGR01257; HMM-score: 89.8)
  Metabolism Transport and binding proteins Unknown substrate anchored repeat-type ABC transporter, ATP-binding subunit (TIGR03771; HMM-score: 87.9)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 81)
  Metabolism Transport and binding proteins Other heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 81)
  Metabolism Central intermediary metabolism Phosphorus compounds phosphonate C-P lyase system protein PhnK (TIGR02323; HMM-score: 79.2)
  Metabolism Transport and binding proteins Other pigment precursor permease (TIGR00955; HMM-score: 76.8)
  ATP-binding cassette protein, ChvD family (TIGR03719; HMM-score: 69.3)
  Metabolism Transport and binding proteins Amino acids, peptides and amines cyclic peptide transporter (TIGR01194; HMM-score: 69.1)
  Metabolism Transport and binding proteins Other cyclic peptide transporter (TIGR01194; HMM-score: 69.1)
  Metabolism Transport and binding proteins Other multi drug resistance-associated protein (MRP) (TIGR00957; HMM-score: 68.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly ATPase SufC (TIGR01978; HMM-score: 66.9)
  Metabolism Transport and binding proteins Other pleiotropic drug resistance family protein (TIGR00956; HMM-score: 45.5)
  Metabolism Transport and binding proteins Anions cystic fibrosis transmembrane conductor regulator (CFTR) (TIGR01271; EC 3.6.3.49; HMM-score: 42.8)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids peroxysomal long chain fatty acyl transporter (TIGR00954; HMM-score: 40.3)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit A (TIGR00630; EC 3.1.25.-; HMM-score: 37.6)
  Metabolism Transport and binding proteins Amino acids, peptides and amines oligopeptide/dipeptide ABC transporter, ATP-binding protein, C-terminal domain (TIGR01727; HMM-score: 12.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone naphthoate synthase (TIGR01929; EC 4.1.3.36; HMM-score: 11.3)
• TheSEED  :
  • Methionine ABC transporter ATP-binding protein
  Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis  Methionine ABC transporter ATP-binding protein
  and 1 more
  Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Degradation  Methionine ABC transporter ATP-binding protein
• PFAM:
  P-loop_NTPase (CL0023) ABC_tran; ABC transporter (PF00005; HMM-score: 114.3)
  and 10 more
  ACT (CL0070) NIL; NIL domain (PF09383; HMM-score: 50.5)
  P-loop_NTPase (CL0023) AAA_22; AAA domain (PF13401; HMM-score: 27.5)
  AAA_21; AAA domain, putative AbiEii toxin, Type IV TA system (PF13304; HMM-score: 26.3)
  AAA_16; AAA ATPase domain (PF13191; HMM-score: 22)
  SMC_N; RecF/RecN/SMC N terminal domain (PF02463; HMM-score: 21.6)
  Zeta_toxin; Zeta toxin (PF06414; HMM-score: 17.4)
  RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 16)
  AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 13.9)
  MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 13.1)
  AAA_24; AAA domain (PF13479; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 1.05
  • Cytoplasmic Membrane Score: 8.78
  • Cellwall Score: 0.08
  • Extracellular Score: 0.09
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010182
  • TAT(Tat/SPI): 0.000889
  • LIPO(Sec/SPII): 0.001392
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161235 NCBI
• RefSeq: YP_493496 NCBI
• UniProt: Q2FII2 UniProt

⊟Protein sequence

• MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGKSTLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKNIMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPTVLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGKVIEQGPVTQVFENPQHTVTKRFVKDDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTTEPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVKMEVLRHG

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0796 > SAUSA300_0797 > SAUSA300_0798

⊟Regulation

• regulator: S-box (transcription termination) regulon

  S-box

  (RNA)

  important in Methionine biosynthesis; regulatory site identified based on RegPrecise data for N315 RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications