PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0834 [new locus tag: SAUSA300_RS04500 ]
• pan locus tag^?: SAUPAN003028000
• symbol: SAUSA300_0834
• pan gene symbol^?: —
• synonym:
• product: D-isomer specific 2-hydroxyacid dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0834 [new locus tag: SAUSA300_RS04500 ]
• symbol: SAUSA300_0834
• product: D-isomer specific 2-hydroxyacid dehydrogenase
• replicon: chromosome
• strand: +
• coordinates: 912873..913832
• length: 960
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914859 NCBI
• RefSeq: YP_493534 NCBI
• BioCyc: see SAUSA300_RS04500
• MicrobesOnline: 1292349 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  TTGGTAAAAATAGTTGTTTCGAGGAAAATTCCAGATAAATTTTATCAACAATTAAGTAAA
  CTTGGTGACGTTGTTATGTGGCAAAAATCATTAGTGCCTATGCCTAAAGATCAATTTGTG
  ACAGCACTTCGTGACGCAGATGCTTGTTTTATTACATTAAGTGAACAGATCGATGCAGAA
  ATTTTAGCGCAATCACCAAATTTAAAAGTAATTGCGAATATGGCTGTAGGATATGACAAC
  ATCGATGTTGAAAGTGCAACAGCGAATAACGTGGTTGTCACGAATACACCAAATGTACTT
  ACTGAAACAACTGCAGAATTAGGATTTACATTAATGCTTGCTATAGCACGCCGTATTGTA
  GAAGCTGAAAAATATGTAGAAGCAGATGCATGGCAAAGCTGGGGTCCTTATTTATTGTCA
  GGTAAAGATGTCTTCAATTCAACTATTGGAATATATGGTATGGGAGATATTGGTAAAGCT
  TTTGCAAGAAGGTTGCAAGGGTTTAATACTAATATTCTTTATCATAATCGATCAAGACAT
  AAAGATGCAGAGGCGGACTTTAATGCAACATATGTTTCTTTTGAAACGTTGTTAGCAGAA
  AGTGATTTTATCATCTGTACAGCGCCACTTACAAAAGAAACACATCATAAATTTAATGCT
  GAAGCATTTGAACAAATGAAAAATGATGCAATTTTTATTAATATCGGTAGAGGACAAATT
  GTAGATGAAACAGCATTAATCGATGCACTAGACAATAAAGAAATTTTAGCATGTGGTTTA
  GATGTATTAGCAAATGAACCGATTGATCATACACATCCATTAATGGGACGTGATAATGTT
  CTGATTACACCACACATTGGTAGCGCATCAGTAACAACACGGGACAATATGATTCAATTA
  TGTATTAATAATATAGAAGCGGTTATGACAAATCAGGTACCACATACTCCAGTAAATTGA
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⊟Protein

⊟General

• locus tag: SAUSA300_0834 [new locus tag: SAUSA300_RS04500 ]
• symbol: SAUSA300_0834
• description: D-isomer specific 2-hydroxyacid dehydrogenase
• length: 319
• theoretical pI: 4.74512
• theoretical MW: 35357.1
• GRAVY: -0.0435737

⊟Function

• TIGRFAM:
  Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 213.3)
  and 2 more
  Metabolism Energy metabolism Amino acids and amines adenosylhomocysteinase (TIGR00936; EC 3.3.1.1; HMM-score: 21.5)
  Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 17.5)
• TheSEED  :
  • Glyoxylate reductase (EC 1.1.1.26)
  • Glyoxylate reductase (NADP+) (EC 1.1.1.79)
  • Hydroxypyruvate reductase (EC 1.1.1.81)
  Carbohydrates Central carbohydrate metabolism Glycolate, glyoxylate interconversions  Glyoxylate reductase (EC 1.1.1.26)
  and 3 more
  Carbohydrates Central carbohydrate metabolism Glycolate, glyoxylate interconversions  Glyoxylate reductase (EC 1.1.1.79)
  Carbohydrates Central carbohydrate metabolism Glycolate, glyoxylate interconversions  Hydroxypyruvate reductase (EC 1.1.1.81)
  Carbohydrates Organic acids Glycerate metabolism  Hydroxypyruvate reductase (EC 1.1.1.81)
• PFAM:
  NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 208.8)
  and 5 more
  Form_Glyc_dh (CL0325) 2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 105.6)
  NADP_Rossmann (CL0063) NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 27.2)
  F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 24)
  AdoHcyase_NAD; S-adenosyl-L-homocysteine hydrolase, NAD binding domain (PF00670; HMM-score: 22.2)
  IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 14.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002252
  • TAT(Tat/SPI): 0.000129
  • LIPO(Sec/SPII): 0.000229
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162234 NCBI
• RefSeq: YP_493534 NCBI
• UniProt: A0A0H2XIS1 UniProt

⊟Protein sequence

• MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALRDADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTETTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKAFARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKFNAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMGRDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0832 > SAUSA300_0833 > SAUSA300_0834

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications