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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0839 [new locus tag: SAUSA300_RS04535 ]
  • pan locus tag?: SAUPAN003035000
  • symbol: SAUSA300_0839
  • pan gene symbol?: nfu
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0839 [new locus tag: SAUSA300_RS04535 ]
  • symbol: SAUSA300_0839
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 918769..919011
  • length: 243
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    ATGCCTACTGAAGATACAACGATGTTTGATCAAGTAGCAGAAGTTATTGAACGTCTTCGT
    CCATTTTTATTACGTGATGGTGGCGACTGCTCATTGATTGACGTGGAAGACGGTATTGTT
    AAATTACAATTACATGGTGCATGTGGTACATGCCCAAGTTCTACAATCACTCTTAAAGCT
    GGTATTGAGCGTGCATTACACGAAGAAGTGCCTGGTGTAATAGAAGTAGAACAAGTATTC
    TAA
    60
    120
    180
    240
    243

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0839 [new locus tag: SAUSA300_RS04535 ]
  • symbol: SAUSA300_0839
  • description: hypothetical protein
  • length: 80
  • theoretical pI: 4.10599
  • theoretical MW: 8740.95
  • GRAVY: 0.08625

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other Fe-S cluster assembly protein NifU (TIGR02000; HMM-score: 57.2)
    Metabolism Central intermediary metabolism Nitrogen fixation Fe-S cluster assembly protein NifU (TIGR02000; HMM-score: 57.2)
    and 1 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other IscR-regulated protein YhgI (TIGR03341; HMM-score: 40)
  • TheSEED  :
    • NifU-C-terminal domain-like protein YutI
    Sulfur Metabolism Organic sulfur assimilation At5g37530 (CsdL protein family)  NifU-like domain protein
  • PFAM:
    NifU (CL0232) NifU; NifU-like domain (PF01106; HMM-score: 92.5)
    and 1 more
    FeS_assembly_P; Iron-sulfur cluster assembly protein (PF01883; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.04606
    • TAT(Tat/SPI): 0.010062
    • LIPO(Sec/SPII): 0.000738
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQLHGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

One of three iron-sulfur cluster carrier proteins (Nfu, SufA and SufT) that can transfer preformed Fe-S clusters to staphylococcal proteins that require Fe-S clusters to function. Although they are partially functionally-redundant, each has a preferred target enzyme (Nfu is preferred in vivo to generate holo-aconitase while SufT is preferred to generate holo-LipA). By altering the relative amount of each carrier protein, the cell can control which target enzymes receive Fe-S clusters when resources are limited.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Ameya A Mashruwala, Christina A Roberts, Shiven Bhatt, Kerrie L May, Ronan K Carroll, Lindsey N Shaw, Jeffrey M Boyd
    Staphylococcus aureus SufT: an essential iron-sulphur cluster assembly factor in cells experiencing a high-demand for lipoic acid.
    Mol Microbiol: 2016, 102(6);1099-1119
    [PubMed:27671355] [WorldCat.org] [DOI] (I p)
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