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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_0874 [new locus tag: SAUSA300_RS04715 ]
- pan locus tag?: SAUPAN003108000
- symbol: SAUSA300_0874
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_0874 [new locus tag: SAUSA300_RS04715 ]
- symbol: SAUSA300_0874
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 958963..959787
- length: 825
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3913533 NCBI
- RefSeq: YP_493574 NCBI
- BioCyc: see SAUSA300_RS04715
- MicrobesOnline: 1292389 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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781ATGCAACCACATTTAATATGTCTAGACTTAGACGGAACATTATTAAACGATAACAAAGAA
ATTTCATCATATACTAAACAAGTATTAAATGAATTACAACAACGTGGACACCAAATTATG
ATTGCGACTGGCAGACCTTATCGTGCAAGTCAAATGTATTATCATGAATTAAATTTAACG
ACACCAATTGTTAATTTTAATGGCGCTTACGTACATCACCCTAAAGATAAAAACTTCAAA
ACTTGCCATGAAATTTTAGATTTAGGCATCGCACAAAACATTATTCAAGGATTACAACAA
TATCAAGTATCGAATATTATAGCAGAAGTGAAAGATTATGTTTTCATTAACAATCATGAT
CCAAGATTATTTGAAGGTTTTTCAATGGGTAATCCAAGAATTCAAACTGGTAATTTACTT
GTCCACTTGAAAGAATCCCCTACCTCAATTTTAATTGAAGCCGAAGAAAGTAAAATACCT
GAAATCAAAAATATGCTTACTCATTTTTATGCCGATCATATTGAGCATCGACGCTGGGGC
GCACCATTCCCTGTCATTGAAATTGTAAAACTTGGTATTAATAAAGCAAGAGGCATTGAG
CAAGTTAGACAATTTTTAAATATTGACCGAAATAATATTATTGCATTCGGTGATGAAGAT
AATGATATTGAAATGATTGAGTACGCGCGTCACGGTGTTGCTATGGAAAATGGTTTGCAA
GAACTTAAAGATGTAGCGAACAATATTACATTCAACAATAATGAAGATGGCATTGGTCGA
TATTTGAATGATTTCTTTAATTTAAATATTAGATATTACTGTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_0874 [new locus tag: SAUSA300_RS04715 ]
- symbol: SAUSA300_0874
- description: hypothetical protein
- length: 274
- theoretical pI: 5.90828
- theoretical MW: 31668.7
- GRAVY: -0.419708
⊟Function[edit | edit source]
- TIGRFAM: Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 205.2)and 16 moreUnknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 93.3)phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 78)sucrose-phosphate phosphatase subfamily (TIGR01482; EC 3.1.3.-; HMM-score: 64.9)mannosyl-3-phosphoglycerate phosphatase family (TIGR01486; EC 3.1.3.-; HMM-score: 51)mannosyl-3-phosphoglycerate phosphatase (TIGR02461; EC 3.1.3.70; HMM-score: 43.9)Unknown function General mannosyl-3-phosphoglycerate phosphatase homolog (TIGR02463; EC 3.1.3.-; HMM-score: 38)sucrose-phosphate synthase, sucrose phosphatase-like domain (TIGR02471; HMM-score: 31.3)Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 29.1)Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 27.1)sucrose phosphatase (TIGR01485; EC 3.1.3.24; HMM-score: 26.8)Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 24)Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 23.9)Unknown function Enzymes of unknown specificity HAD hydrolase, family IB (TIGR01490; HMM-score: 19.6)HAD phosphatase, family IIIC (TIGR01681; HMM-score: 16.6)Unknown function Enzymes of unknown specificity HAD hydrolase, family IIA (TIGR01460; HMM-score: 16.3)phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 14.5)
- TheSEED :
- 5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase (EC 3.1.3.104)
- PFAM: HAD (CL0137) Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 216.4)and 10 moreS6PP; Sucrose-6F-phosphate phosphohydrolase (PF05116; HMM-score: 50.9)Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 27.4)HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 25.8)HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 24.2)Trehalose_PPase; Trehalose-phosphatase (PF02358; HMM-score: 19.9)Acid_PPase; Acid Phosphatase (PF12689; HMM-score: 18.8)Hydrolase_6; haloacid dehalogenase-like hydrolase (PF13344; HMM-score: 16.9)DUF2608; Protein of unknown function (DUF2608) (PF11019; HMM-score: 13.9)GT-B (CL0113) Glyco_trans_4_4; Glycosyl transferase 4-like domain (PF13579; HMM-score: 12.2)HAD (CL0137) Acid_phosphat_B; HAD superfamily, subfamily IIIB (Acid phosphatase) (PF03767; HMM-score: 11)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.00715
- TAT(Tat/SPI): 0.000497
- LIPO(Sec/SPII): 0.001067
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATGRPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQVSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIPEIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGDEDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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