PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0981 [new locus tag: SAUSA300_RS05280 ]
• pan locus tag^?: SAUPAN003301000
• symbol: SAUSA300_0981
• pan gene symbol^?: rlmQ
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0981 [new locus tag: SAUSA300_RS05280 ]
• symbol: SAUSA300_0981
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1076325..1077497
• length: 1173
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914940 NCBI
• RefSeq: YP_493679 NCBI
• BioCyc: see SAUSA300_RS05280
• MicrobesOnline: 1292496 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAAAATAGCAACTCTGAACAAAGGCAAAGAAACAAAATATTTTAATGGATATCCTTTA
  ATTGAAGAAGAGGATATCTATTCACAAGATCATTTAAAAGAAGGAGATATTTTTCAAATT
  GTGACTGATAAATCACAATATGTCGCAACGGCTTATGTTGGTCGCCAACATAAAGGATTA
  GGTTGGGTTCTAACATACGATAAAGCTCAACAAATCAACACAGCTTTCTTTGTAAAATTG
  TTTAATACTGCATTAGCAGAACGTGATTATTATTTTAATATAGATGGAACAAATGCTTTT
  AGATTATTTAATGCTGAAGGTGATGGTGTTGGGGGATTAACAATCGACAATTACGATGGT
  CATTTGTTGATTCAATGGTACTCAAAAGGTATTTATAAATTTAAATATGCCATTCTTGAA
  GCGGTTAGAAAAGTATTTGATTATAAATCTATTTACGAAAAAGTAAGATTTAAAGACAGC
  GAATATAGTGGTGGTTTTGTTGAAGGAGATGCACCTGAGTTTCCAATTGTTATCGAAGAA
  AACTTCACATTTTATAATGTAGACCTTGAAGATGGTTTGATGACAGGTATCTTTTTAGAT
  CAAAAAGAAGTGCGCAAGAAATTAAGGGATCAATATGCCAAAGAACGCCATGTTTTAAAC
  TTATTTAGTTATACAGGTGCTTTTTCTGCAATAGCAGCAAGTGAGGCATCTTCAACAACA
  AGTGTAGATTTGGCTAATCGTTCTCGTAGTTTAACTGAAGAAAATTTTGGATTAAATGCT
  ATTGATCCTAAATCCCAATATATTTATGTCATGGACACTTTTGATTTCTATAAATATGCT
  GCACGACATGGACATAGTTATGACACGATCGTGATTGATCCACCTAGCTTTGCGCGTAAC
  AAAAAACGTACATTTTCAGTGCAAAAAGATTATGACAAATTAATTAATGGCGCCTTAAAT
  ATCTTATCATCTGAAGGAACATTATTGTTATGTACAAACGCAAGTGTATATCCATTAAAG
  CAATTTAAAAATACTATTAAAAAGACGCTTGAAGAGAGTGGCGTTGATTATGAATTAACT
  GAAGTTATGGGATTACCAAAAGATTTTAAAACGCATCCACATTATAAGCCATCTAAATAT
  TTAAAAGCTGTTTTTGTAAATATTAGACATTAA
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  1173
  

⊟Protein

⊟General

• locus tag: SAUSA300_0981 [new locus tag: SAUSA300_RS05280 ]
• symbol: SAUSA300_0981
• description: hypothetical protein
• length: 390
• theoretical pI: 6.80741
• theoretical MW: 44767.2
• GRAVY: -0.425128

⊟Function

• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD (TIGR00095; EC 2.1.1.171; HMM-score: 20.5)
  and 1 more
  FkbH domain (TIGR01686; HMM-score: 11.2)
• TheSEED  :
  • LSU m5C1962 methyltransferase RlmI
  Protein Metabolism Protein biosynthesis Ribosome biogenesis bacterial  LSU m5C1962 methyltransferase RlmI
• PFAM:
  NADP_Rossmann (CL0063) Methyltrans_SAM; S-adenosylmethionine-dependent methyltransferase (PF10672; HMM-score: 86.3)
  and 5 more
  Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 26.9)
  MTS; Methyltransferase small domain (PF05175; HMM-score: 25)
  Met_10; Met-10+ like-protein (PF02475; HMM-score: 18.3)
  N6-adenineMlase; Probable N6-adenine methyltransferase (PF10237; HMM-score: 17.3)
  PUA (CL0178) YTH; YT521-B-like domain (PF04146; HMM-score: 13)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004144
  • TAT(Tat/SPI): 0.000136
  • LIPO(Sec/SPII): 0.000446
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159894 NCBI
• RefSeq: YP_493679 NCBI
• UniProt: A0A0H2XDU9 UniProt

⊟Protein sequence

• MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDKSQYVATAYVGRQHKGLGWVLTYDKAQQINTAFFVKLFNTALAERDYYFNIDGTNAFRLFNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDSEYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHVLNLFSYTGAFSAIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDFYKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNASVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0981 > SAUSA300_0982

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications