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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1088 [new locus tag: SAUSA300_RS05895 ]
  • pan locus tag?: SAUPAN003469000
  • symbol: SAUSA300_1088
  • pan gene symbol?:
  • synonym:
  • product: glyoxalase family protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1088 [new locus tag: SAUSA300_RS05895 ]
  • symbol: SAUSA300_1088
  • product: glyoxalase family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1190120..1190917
  • length: 798
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGTATCATAACAGTAACGCAAACTTTGTCAATGGTATCACTTTAAATGTGAGAGATAAG
    AATGAATTAAAGCCATTTTATGAGGACATATTAGGATTAAATATTATAAATGAGACATTA
    ACATCGATACAATATGAAGTAGGTCAAAATAATCATGTCATTACACTTGTTGAATTACAA
    AATGGACGTGAACCTTTAATGTCCGAAGCGGGACTGTTTCATATCGCAATTAAACTACCT
    CAAATTAGTGATTTAGCTAATTTACTAATTCATTTAAGCGAATATGATATTCCAGTTAAC
    GGAGGTATACAGCCTGCTTCGTTATCATTATTTTTTGAAGACCCGGAAGGAAACGGTTTT
    AAATTTTATGTTGATAAAGACGAAGCGCAATGGACGAGGCAAAATAATTTAGTAAAAATT
    GATATTAGACCATTAAATGTACCGAGATTAGTGAGTCATGCAACAAAATTGTTATGGTTA
    GGTATTCCAGATGACGCTATTATAGGTGCATTGCATATTAAGACAATTCATTTATCAGAG
    GTAAAAGAGTACTACCTCGATTATTTTGGATTAGAGCAATCGGCATATATGGATGATTAT
    TCAATATTTTTAGCATCGAATGGCTATTATCAACATTTGGCCATGAATGATTGGGTATCA
    GCAACGAAACGTGTAGAAAATTTTGATACGTATGGATTAGCAATTGTTGACTTTCATTAT
    CCTGAAACAACACATTTAAATTTACAAGGTCCGGATGGTATCTATTATCGCTTTAATCAT
    ATCGAAGTTGAAGATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    798

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1088 [new locus tag: SAUSA300_RS05895 ]
  • symbol: SAUSA300_1088
  • description: glyoxalase family protein
  • length: 265
  • theoretical pI: 4.54898
  • theoretical MW: 30367
  • GRAVY: -0.221132

Function[edit | edit source]

  • reaction:
    EC 4.4.1.5?  ExPASy
    Lactoylglutathione lyase (R)-S-lactoylglutathione = glutathione + methylglyoxal
  • TIGRFAM:
    Metabolism Energy metabolism Other catechol 2,3 dioxygenase (TIGR03211; EC 1.13.11.2; HMM-score: 21.8)
    3,4-dihydroxyphenylacetate 2,3-dioxygenase (TIGR02295; EC 1.13.11.15; HMM-score: 20.6)
  • TheSEED  :
    • Predicted ring-cleavage extradiol dioxygenase
  • PFAM:
    Glyoxalase (CL0104) Glyoxalase; Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily (PF00903; HMM-score: 40.3)
    and 2 more
    Glyoxalase_2; Glyoxalase-like domain (PF12681; HMM-score: 16.5)
    no clan defined CppA_C; CppA C-terminal (PF14507; HMM-score: 15)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004102
    • TAT(Tat/SPI): 0.000121
    • LIPO(Sec/SPII): 0.000942
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MYHNSNANFVNGITLNVRDKNELKPFYEDILGLNIINETLTSIQYEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGGIQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNNLVKIDIRPLNVPRLVSHATKLLWLGIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDWVSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]