PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1232 [new locus tag: SAUSA300_RS06680 ]
• pan locus tag^?: SAUPAN003707000
• symbol: SAUSA300_1232
• pan gene symbol^?: katA
• synonym:
• product: catalase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1232 [new locus tag: SAUSA300_RS06680 ]
• symbol: SAUSA300_1232
• product: catalase
• replicon: chromosome
• strand: +
• coordinates: 1349997..1351514
• length: 1518
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914723 NCBI
• RefSeq: YP_493929 NCBI
• BioCyc: see SAUSA300_RS06680
• MicrobesOnline: 1292747 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGTCACAACAAGACAAAAAGTTAACTGGTGTTTTTGGGCATCCAGTATCAGACCGAGAA
  AATAGTATGACAGCAGGGCCTAGGGGACCTCTTTTAATGCAAGATATTTACTTTTTAGAG
  CAAATGTCTCAATTTGATAGAGAAGTAATACCAGAACGTCGAATGCATGCCAAAGGTTCT
  GGTGCATTTGGGACATTTACTGTAACTAAAGATATAACAAAATATACGAATGCTAAAATA
  TTCTCTGAAATAGGTAAGCAAACCGAAATGTTTGCCCGTTTCTCTACTGTAGCAGGAGAA
  CGTGGTGCTGCTGATGCGGAGCGTGACATTCGAGGATTTGCGTTAAAGTTCTACACTGAA
  GAAGGGAACTGGGATTTAGTAGGGAATAACACACCAGTATTCTTCTTTAGAGATCCAAAG
  TTATTTGTTAGTTTAAATCGTGCGGTGAAACGAGATCCTAGAACAAATATGAGAGATGCA
  CAAAATAACTGGGATTTCTGGACGGGTCTTCCAGAAGCATTGCACCAAGTAACGATCTTA
  ATGTCAGATAGAGGGATTCCTAAAGATTTACGTCATATGCATGGGTTCGGTTCTCACACA
  TACTCTATGTATAATGATTCTGGTGAACGTGTTTGGGTTAAATTCCATTTTAGAACGCAA
  CAAGGTATTGAAAACTTAACTGATGAAGAAGCTGCTGAAATTATAGCTACAGATCGTGAT
  TCATCTCAACGCGATTTATTCGAAGCCATTGAAAAAGGTGATTATCCAAAATGGACAATG
  TATATTCAAGTAATGACTGAGGAACAAGCTAAAAACCATAAAGATAATCCATTTGATTTA
  ACAAAAGTATGGTATCACGATGAGTATCCTCTAATTGAAGTTGGAGAGTTTGAATTAAAT
  AGAAATCCAGATAATTACTTTATGGATGTTGAACAAGCTGCGTTTGCACCAACTAATATT
  ATTCCAGGATTAGATTTTTCTCCAGACAAAATGCTGCAAGGGCGTTTATTCTCATATGGC
  GATGCGCAAAGATATCGATTAGGAGTTAATCATTGGCAGATTCCTGTAAACCAACCTAAA
  GGTGTTGGTATTGAAAATATTTGTCCTTTTAGTAGAGATGGTCAAATGCGCGTAGTTGAC
  AATAACCAAGGTGGAGGAACACATTATTATCCAAATAACCATGGTAAATTTGATTCTCAA
  CCTGAATATAAAAAGCCACCATTCCCAACTGATGGATACGGCTATGAATATAATCAACGT
  CAAGATGATGATAATTATTTTGAACAACCAGGTAAATTGTTTAGATTACAATCAGAGGAC
  GCTAAAGAAAGAATTTTTACAAATACAGCAAATGCAATGGAAGGCGTAACGGATGATGTT
  AAACGACGTCATATTCGTCATTGTTACAAAGCTGACCCAGAATATGGTAAAGGTGTTGCA
  AAAGCATTAGGTATTGATATAAATTCTATTGATCTTGAAACTGAAAATGATGAAACATAC
  GAAAACTTTGAAAAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1232 [new locus tag: SAUSA300_RS06680 ]
• symbol: SAUSA300_1232
• description: catalase
• length: 505
• theoretical pI: 5.13252
• theoretical MW: 58379.5
• GRAVY: -0.845743

⊟Function

• reaction:
  EC 1.11.1.6?  ExPASy
  Catalase 2 H₂O₂ = O₂ + 2 H₂O
• TIGRFAM:
• TheSEED  :
  • Catalase KatE (EC 1.11.1.6)
  Stress Response Oxidative stress Oxidative stress  Catalase (EC 1.11.1.6)
  and 1 more
  Stress Response Oxidative stress Protection from Reactive Oxygen Species  Catalase (EC 1.11.1.6)
• PFAM:
  no clan defined Catalase; Catalase (PF00199; HMM-score: 607)
  and 1 more
  Catalase-rel; Catalase-related immune-responsive (PF06628; HMM-score: 80.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: heme
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.035586
  • TAT(Tat/SPI): 0.006403
  • LIPO(Sec/SPII): 0.001321
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160511 NCBI
• RefSeq: YP_493929 NCBI
• UniProt: Q2FH99 UniProt

⊟Protein sequence

• MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQFDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERGAADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDAQNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFRTQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDNPFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQGRLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPNNHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTANAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulators: PerR* (repression) regulon, CodY (repression) regulon

  PerR*	(TF)	important in Oxidative stress response; RegPrecise
  CodY	(TF)	important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications