From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1256 [new locus tag: SAUSA300_RS06825 ]
  • pan locus tag?: SAUPAN003750000
  • symbol: msrA
  • pan gene symbol?: msrA2
  • synonym:
  • product: methionine sulfoxide reductase A

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1256 [new locus tag: SAUSA300_RS06825 ]
  • symbol: msrA
  • product: methionine sulfoxide reductase A
  • replicon: chromosome
  • strand: -
  • coordinates: 1384179..1384688
  • length: 510
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAATATTAATACAGCTTATTTTGCCGGAGGTTGCTTTTGGTGTATGACGAAACCATTT
    GACACCTTTGACGGCATAGAAAAAGTAACTTCTGGATATATGGGCGGACATATTGAAAAT
    CCTACTTACGAACAAGTAAAATCAGGTACGAGTGGTCATTTAGAAACTGTTGAAATTCAA
    TATGATGTTGCATTATTCTCATACAATAAGTTATTAGAAATATTTTTCTCAGTCATTGAC
    CCATTAGATACAGGTGGTCAATATCAAGACCGTGGTCCTCAATATCAAACAGCTATTTTC
    TACACTAATGATCATCAAAAAGAACTCGCTGAGACTTATATCGAGCAGCTTAAAAATACG
    ATTAATGCTGATAAGGCAATTGCAACAAAAATATTACCAGCGTCACAATTTTACAAAGCC
    GAAGACTATCACCAAGATTTTTATAAGAAAAATCCAGAGCGCTATGCAGAAGAACAAAAA
    ATACGCCAAGAATACAAAAATAAGCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    510

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1256 [new locus tag: SAUSA300_RS06825 ]
  • symbol: MsrA
  • description: methionine sulfoxide reductase A
  • length: 169
  • theoretical pI: 4.86349
  • theoretical MW: 19610.7
  • GRAVY: -0.739645

Function[edit | edit source]

  • reaction:
    EC 1.8.4.11?  ExPASy
    Peptide-methionine (S)-S-oxide reductase Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin
  • TIGRFAM:
    Cellular processes Cellular processes Adaptations to atypical conditions peptide-methionine (S)-S-oxide reductase (TIGR00401; EC 1.8.4.11; HMM-score: 188.7)
    Genetic information processing Protein fate Protein modification and repair peptide-methionine (S)-S-oxide reductase (TIGR00401; EC 1.8.4.11; HMM-score: 188.7)
  • TheSEED  :
    • Peptide-methionine (S)-S-oxide reductase MsrA (EC 1.8.4.11)
    Protein Metabolism Protein processing and modification Peptide methionine sulfoxide reductase  Peptide methionine sulfoxide reductase MsrA (EC 1.8.4.11)
  • PFAM:
    no clan defined PMSR; Peptide methionine sulfoxide reductase (PF01625; HMM-score: 198.7)
    and 2 more
    RCDG1; Renal cancer differentiation gene 1 protein (PF15725; HMM-score: 14.4)
    DUF4298; Domain of unknown function (DUF4298) (PF14131; HMM-score: 13)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.047787
    • TAT(Tat/SPI): 0.000356
    • LIPO(Sec/SPII): 0.012765
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYEQVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYTNDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQKIRQEYKNKQ

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]