PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1278 [new locus tag: SAUSA300_RS06940 ]
• pan locus tag^?: SAUPAN003790000
• symbol: pepF
• pan gene symbol^?: —
• synonym:
• product: oligoendopeptidase F

⊟Additional information (user-provided)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1278 [new locus tag: SAUSA300_RS06940 ]
• symbol: pepF
• product: oligoendopeptidase F
• replicon: chromosome
• strand: +
• coordinates: 1405803..1407617
• length: 1815
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913482 NCBI
• RefSeq: YP_493975 NCBI
• BioCyc: see SAUSA300_RS06940
• MicrobesOnline: 1292793 MicrobesOnline

⊟Phenotype

⊟Additional information (user-provided)

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  
  ATGTCTCAAGGTTTACCTTTAAGAGAAGATGTTCCTGTTTCAGAAACATGGGATTTAGTA
  GACTTATTTAAAGATGATCAACAATATTATGAAAGTATTGACGCTCTAGTACAACAAGCA
  AATCAATTTCATCATACATATGCAACAACATTAAATTCAATCGAACAAATTAATACTGCT
  TTAGCTGAATTAGAAAATATTTTAATTGCCTTAGATCGCTTAAGTAATTATGCAGAACTA
  CGTTTAAGTGTAGATACTAGTAATATCGAGGCACAAGTATTGAGCGCTAAATTATCTACT
  ACATACGGTAAAATTGTTAGCCAATTATCATTTGTAGAGTCAGAAATACTTGAATTACCA
  GAAGAAATACTTCAACAATTAGAAGAATCATGTCCATATCAACACTATATTAAACAGTTA
  ATAAAACAAAAGCCATTCCAATTATCTGCGTCGGTAGAACAAGTATTAGCAACTTTATCA
  CCTACGCTAAACAGTCCTTACGATTTATACGGCACGACAAAAATGCTAGATATTACATTC
  GATTCATTTGAACATGATGGTACAACGTACCCTGTCGACTATGCTACGTTTGAAAATGAT
  TATGAAGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGG
  ATTCGAAAATATCAGCATACTACCGCGGCTACATATAATATGCAAGTACAACAAGAAAAA
  ATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCGATTATTTATTACATAGTCAAGAA
  GTAACGCGTGATATGTTTGACCGTCAAATCGATATGATTATGCGTGACTTGGCACCAGTT
  ATGCAGAAATATGCTAAACTTTTACAACGTATTCACGGATTAGATAACATGCGTTTTGAA
  GACTTGAAGATTTCTGTAGACCCTGATTATGAACCAGAGATTTCAATTGAAGACTCAAAA
  AATTATATTTTCGGTGCGTTAAGTGTTTTAGGTGATGACTATACAAACATGTTACGTGAA
  GCATACGATCAGCGATGGATTGATTTTGCACAAAATAAAGGTAAAGATACAGGCGCATTT
  TGTGCAAGTCCATACTTTACACATTCATATGTGTTTATTTCTTGGACTGGTAAAATGGCT
  GAAGCATTTGTCTTAGCACATGAATTAGGTCATGCAGGTCATTTTACATTAGCTCAAAAA
  CATCAACCATATCTTGAATCAGAAGCATCAATGTACTTTGTTGAAGCCCCTTCTACAATG
  AATGAAATGTTGATGGCCAATTATTTATTTAACACAAGTGATAATCCAAGATTTAAGCGT
  TGGGTTATTGGCTCAATTTTATCTAGAACATATTATCATAATATGGTTACCCATTTATTA
  GAAGCTGCTTATCAACGTGAAGTGTATCACAAAGTAGATCAAGGTGAATCTTTAAATGCG
  CCGACATTAAATGAAATAATGCTAAATGTTTATAAACAATTTTTTGGAGATGCAGTAGAC
  ATGACTGAGGGTGCTGAATTAACATGGATGCGTCAACCTCATTACTATATGGGATTATAT
  TCGTATACGTATTCTGCTGGCTTAACAATCGGAACTGTCGTTTCTCAAAAGATTAAAAAT
  GAAGGCCAACCAGCTGTTGATGCTTGGTTAGAAACATTGAAAAAAGGTGGTAGTGTATCA
  CCTGTCGAACTTGCAAACATTGCAGGTGTAGACATTACTACAGAACAGCCACTTAAATCT
  ACAATTCAATATATTTCTGATTTAGTCGATGAAGTTGAAAAATTAACAGATGAAATTGAG
  CAAGCAAATAACTAA
  60
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  1140
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  1260
  1320
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  1560
  1620
  1680
  1740
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  1815
  

⊟Protein

⊟General

• locus tag: SAUSA300_1278 [new locus tag: SAUSA300_RS06940 ]
• symbol: PepF
• description: oligoendopeptidase F
• length: 604
• theoretical pI: 4.38757
• theoretical MW: 69284.2
• GRAVY: -0.402152

⊟Function

• reaction:
  EC 3.4.24.-?  ExPASy
• TIGRFAM:
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides oligoendopeptidase F (TIGR00181; EC 3.4.24.-; HMM-score: 720.9)
  and 2 more
  oligoendopeptidase, pepF/M3 family (TIGR02290; EC 3.4.24.-; HMM-score: 282.3)
  oligoendopeptidase, M3 family (TIGR02289; EC 3.4.24.-; HMM-score: 66.6)
• TheSEED  :
  • Oligoendopeptidase F-like protein
• PFAM:
  Peptidase_MA (CL0126) Peptidase_M3; Peptidase family M3 (PF01432; HMM-score: 139.2)
  and 6 more
  no clan defined Peptidase_M3_N; Oligopeptidase F (PF08439; HMM-score: 53.8)
  GIDA_C_1st; tRNA modifying enzyme MnmG/GidA C-terminal helical domain (PF21680; HMM-score: 14.9)
  BLOC1_2; Biogenesis of lysosome-related organelles complex-1 subunit 2 (PF10046; HMM-score: 14)
  Peptidase_MA (CL0126) Peptidase_M78; IrrE N-terminal-like domain (PF06114; HMM-score: 13.8)
  no clan defined DUF5798; Family of unknown function (DUF5798) (PF19111; HMM-score: 10)
  LOH1CR12; Tumour suppressor protein (PF10158; HMM-score: 9.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8878
  • Cytoplasmic Membrane Score: 0.0267
  • Cell wall & surface Score: 0.0015
  • Extracellular Score: 0.0841
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001997
  • TAT(Tat/SPI): 0.000547
  • LIPO(Sec/SPII): 0.000272
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161627 NCBI
• RefSeq: YP_493975 NCBI
• UniProt: A0A0H2XIY5 UniProt

⊟Additional information (user-provided)

⊟Protein sequence

• MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFHHTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTYGKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLSPTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFSDGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRDLAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDYTNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHAGHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRTYYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELTWMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELANIAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Additional information (user-provided)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Additional information (user-provided)

⊟Other information (user-provided)

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications