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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1316 [new locus tag: SAUSA300_RS07170 ]
  • pan locus tag?: SAUPAN003854000
  • symbol: msrB
  • pan gene symbol?: msrB
  • synonym:
  • product: methionine sulfoxide reductase B

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1316 [new locus tag: SAUSA300_RS07170 ]
  • symbol: msrB
  • product: methionine sulfoxide reductase B
  • replicon: chromosome
  • strand: -
  • coordinates: 1448836..1449264
  • length: 429
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGCTTAAAAAAGATAAAAGTGAACTAACAGATATAGAATATATTGTTACACAAGAAAAC
    GGCACTGAACCACCATTTATGAATGAATATTGGAATCATTTTGCTAAAGGAATTTATGTA
    GATAAAATTTCTGGTAAACCTTTATTTACATCTGAAGAAAAGTTTCATTCTGAATGTGGA
    TGGCCTAGCTTTTCCAAAGCGCTTGATGACGATGAAATTATAGAATTAGTCGACAAATCA
    TTTGGTATGTTGAGAACTGAAGTGCGTTCAGAAGAATCAAATAGTCATTTAGGACATGTC
    TTTAATGATGGACCTAAAGAAAGTGGCGGCTTAAGATACTGTATCAATTCCGCTGCAATT
    CAATTTATTCCATATGAAAAGTTAGAAGAATTGGGTTATGGCGATTTAATATCACATTTT
    GATAAGTAG
    60
    120
    180
    240
    300
    360
    420
    429

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1316 [new locus tag: SAUSA300_RS07170 ]
  • symbol: MsrB
  • description: methionine sulfoxide reductase B
  • length: 142
  • theoretical pI: 4.49255
  • theoretical MW: 16277.1
  • GRAVY: -0.601408

⊟Function[edit | edit source]

  • reaction:
    EC 1.8.4.11?  ExPASy
    Peptide-methionine (S)-S-oxide reductase Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin
    EC 1.8.4.12?  ExPASy
    Peptide-methionine (R)-S-oxide reductase Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (R)-S-oxide + thioredoxin
  • TIGRFAM:
    Cellular processes Cellular processes Adaptations to atypical conditions methionine-R-sulfoxide reductase (TIGR00357; EC 1.8.4.-; HMM-score: 191.5)
    Genetic information processing Protein fate Protein modification and repair methionine-R-sulfoxide reductase (TIGR00357; EC 1.8.4.-; HMM-score: 191.5)
  • TheSEED  :
    • Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12)
    Protein Metabolism Protein processing and modification Peptide methionine sulfoxide reductase  Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12)
  • PFAM:
    Beta-tent (CL0080) SelR; SelR domain (PF01641; HMM-score: 158.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.017153
    • TAT(Tat/SPI): 0.00034
    • LIPO(Sec/SPII): 0.00047
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKISGKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFNDGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

During oxidative conditions, such as those experienced within a macrophage, methionine residues in proteins may be oxidized to methionine sulfoxide. Peptide-methionine-(R)-sulfoxide reductase B (MsrB) specifically reduces the (R) epimer of methionine sulfoxide to methionine. Peptide-methionine-(S)-sulfoxide reductase A (MsrA1) catalyzes the analogous reduction for the (S) epimer. S. aureus produces three isoforms of MsrA but only MsrA1 is thought to contribute meaningful reduction of (S)-sulfoxides. While MsrA2 and MsrA3 are catalytically active, they are transcribed at such low levels that they do not make a meaningful contribution under oxidative conditions.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Vineet K Singh, Manisha Vaish, Trintje R Johansson, Kyle R Baum, Robert P Ring, Saumya Singh, Sanjay K Shukla, Jackob Moskovitz
    Significance of four methionine sulfoxide reductases in Staphylococcus aureus.
    PLoS One: 2015, 10(2);e0117594
    [PubMed:25680075] [WorldCat.org] [DOI] (I e)
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