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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_1368 [new locus tag: SAUSA300_RS07470 ]
- pan locus tag?: SAUPAN003943000
- symbol: ansA
- pan gene symbol?: ansA
- synonym:
- product: L-asparaginase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_1368 [new locus tag: SAUSA300_RS07470 ]
- symbol: ansA
- product: L-asparaginase
- replicon: chromosome
- strand: +
- coordinates: 1534595..1535563
- length: 969
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3914781 NCBI
- RefSeq: YP_494065 NCBI
- BioCyc: see SAUSA300_RS07470
- MicrobesOnline: 1292883 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961ATGAAACATCTACTTGTTATTCATACTGGTGGCACCATTAGTATGTCACAAGACCAATCT
AATAAAGTAGTAACAAATGATATCAACCCTATTTCAATGCATCAAGATGTCATAAATCAA
TATGCACAAATAGATGAATTAAATCCTTTTAATGTACCATCACCTCATATGACAATCCAA
CATGTTAAACAATTAAAGGATATTATTTTAGAAGCAGTAACAAATAAATATTATGATGGT
TTCGTTATCACGCATGGTACCGATACGTTAGAAGAAACTGCCTTTTTACTTGATTTAATA
TTAGGTATCGAGCAACCTGTTGTTATTACTGGCGCAATGCGCTCGTCTAATGAAATTGGT
TCTGACGGATTATATAATTATATTTCCGCTATTCGCGTTGCCTCTGATGAAAAGGCCCGT
CATAAAGGCGTGATGGTTGTATTTAATGATGAAATTCATACGGCGCGTAATGTTACCAAA
ACACATACGTCTAATACAAACACATTTCAAAGTCCAAATCATGGTCCGCTAGGTGTATTG
ACAAAGGATCGTGTGCAATTCCATCATATGCCATATCGCCAACAAGCATTGGAAAATGTC
AATGACAAACTAAATGTACCATTAGTAAAAGCATATATGGGTATGCCAGGTGACATTTTT
AGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAGCGTTAGGACAAGGCAAC
ATACCTCCAAGCGCATTAGAAGGCATTCAACAATTAGTATCTTTAAATATACCTATTGTG
CTAGTTTCACGTTCCTTTAATGGTATTGTGAGTCCAACTTACGCATACGATGGTGGTGGT
TACCAACTCGCACAACAAGGTTTTATTTTTTCTAACGGCTTGAATGGTCCAAAAGCAAGA
TTAAAATTATTAGTCGCGTTAAGCAACAATTTAGATAAAGCTGAAATCAAATCATATTTT
GAATTATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_1368 [new locus tag: SAUSA300_RS07470 ]
- symbol: AnsA
- description: L-asparaginase
- length: 322
- theoretical pI: 6.3723
- theoretical MW: 35578.3
- GRAVY: -0.150932
⊟Function[edit | edit source]
- reaction: EC 3.5.1.1? ExPASyAsparaginase L-asparagine + H2O = L-aspartate + NH3
- TIGRFAM: Energy metabolism Amino acids and amines L-asparaginase, type II (TIGR00520; EC 3.5.1.1; HMM-score: 234.8)and 2 moreL-asparaginase, type I (TIGR00519; EC 3.5.1.1; HMM-score: 176.5)Protein synthesis tRNA aminoacylation glutamyl-tRNA(Gln) amidotransferase, subunit D (TIGR02153; EC 6.3.5.-; HMM-score: 131.8)
- TheSEED :
- L-asparaginase (EC 3.5.1.1)
- PFAM: no clan defined Asparaginase; Asparaginase, N-terminal (PF00710; HMM-score: 201)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.67
- Cytoplasmic Membrane Score: 0.01
- Cellwall Score: 0.15
- Extracellular Score: 0.17
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.037167
- TAT(Tat/SPI): 0.000591
- LIPO(Sec/SPII): 0.02003
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQIDELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILGIEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTKTHTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGDIFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAYDGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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