COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1383 [new locus tag: SAUSA300_RS07550 ]
• pan locus tag^?:
• symbol: SAUSA300_1383
• pan gene symbol^?: —
• synonym:
• product: phiSLT ORF484-like protein, lysin

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1383 [new locus tag: SAUSA300_RS07550 ]
• symbol: SAUSA300_1383
• product: phiSLT ORF484-like protein, lysin
• replicon: chromosome
• strand: -
• coordinates: 1548477..1549931
• length: 1455
• essential: unknown

⊟Accession numbers

• Gene ID: 3912912 NCBI
• RefSeq: YP_494080 NCBI
• BioCyc: see SAUSA300_RS07550
• MicrobesOnline: 1292898 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1321
  1381
  1441
  
  ATGTTGATAACAAAAAACCAAGCAGAAAAATGGTTTGATAATTCATTAGGGAAGCAGTTC
  AATCCTGATTTGTTTTATGGATTTCAGTGTTATGATTACGCCAATATGTTCTTTATGTTA
  GCGACAGGCGAAAGGCTGCAAGGTTTATATGCTTATAATATCCCGTTTGATAATAAAGCA
  AAGATTGAAAAATATGGTCAAATAATTAAAAACTATGACAGCTTTTTACCGCAAAAGTTG
  GATATTGTCGTTTTCCCGTCAAAGTATGGTGGCGGAGCTGGACACGTTGAAATTGTTGAG
  AGCGCAAATTTAAACACTTTCACATCATTTGGTCAAAACTGGAACGGTAAAGGTTGGACT
  AATGGCGTTGCGCAACCTGGTTGGGGTCCTGAAACTGTGACAAGACATGTTCATTATTAT
  GACAATCCAATGTATTTTATTAGGTTAAACTTCCCTAACAACTTAAGCGTTGGCAATAAA
  GCTAAAGGTATTATTAAGCAAGCGACTACAAAAAAAGAGGCAGTAATTAAACCTAAAAAA
  ATTATGCTTGTAGCCGGTCATGGTTATAACGATCCTGGAGCAGTAGGAAACGGAACAAAC
  GAACGCGATTTTATACGTAAATATATAACGCCTAATATCGCTAAGTATTTAAGACATGCA
  GGACATGAAGTTGCATTATACGGTGGCTCAAGTCAATCACAAGATATGTATCAAGATACT
  GCATACGGTGTTAATGTAGGCAATAAAAAAGATTATGGCTTATATTGGGTTAAATCACAG
  GGGTATGACATTGTTCTAGAAATACATTTAGACGCAGCAGGAGAAAGCGCAAGTGGTGGG
  CATGTTATTATCTCAAGTCAATTCAATGCAGATACTATTGATAAAAGTATACAAGATGTT
  ATTAAAAATAATTTAGGACAAATAAGAGGTGTAACACCTCGTAACGATTTACTAAATGTT
  AATGTATCAGCAGAAATAAATATCAATTATCGCTTATCTGAATTAGGTTTTATTACTAAT
  AAAAATGATATGGATTGGATTAAGAAAAATTACGACTTGTACTCTAAACTAATAGCTGGT
  GCGATTCATGGTAAGCCAATTGGTGGAGTGGTAGCTAGTGAGGTCAAAGCGCCAGTTAAA
  AACGAAAAGAATCCGCCAGTGCCAGCAGGTTATACACTCGATAAGAATAATGTCCCTTAT
  AAAAAAGAACAAGGCAATTACACAGTAGCTAATGTTAAAGGTAATAATGTAAGAGACGGT
  TATTCAACTAATTCAAGAATTACAGGGGTATTACCCAACAACACAACAATTACGTATGAC
  GGTGCATATTGTATTAATGGTTATAGATGGATTACTTATATTGCTAATAGTGGACAACGT
  CGCTATATTGCGACCGGAGAGGTAGACATAGCAGGCAACCGAATAAGCAGTTTTGGTAAG
  TTTAGTGCAGTTTGA
  60
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  1455
  

⊟Protein

⊟General

• locus tag: SAUSA300_1383 [new locus tag: SAUSA300_RS07550 ]
• symbol: SAUSA300_1383
• description: phiSLT ORF484-like protein, lysin
• length: 484
• theoretical pI: 9.50298
• theoretical MW: 53780.2
• GRAVY: -0.456818

⊟Function

• reaction:
  EC 3.5.1.28?  ExPASy
  N-acetylmuramoyl-L-alanine amidase Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan N-acetylmuramoyl-L-alanine amidase CwlD (TIGR02883; EC 3.5.1.28; HMM-score: 43.9)
  Cellular processes Cellular processes Sporulation and germination N-acetylmuramoyl-L-alanine amidase CwlD (TIGR02883; EC 3.5.1.28; HMM-score: 43.9)
• TheSEED  :
  • Phage endolysin CHAP endopeptidase
  • Phage endolysin N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
• PFAM:
  Peptidase_MH (CL0035) Amidase_3; N-acetylmuramoyl-L-alanine amidase (PF01520; HMM-score: 136.8)
  and 3 more
  Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 36.6)
  SH3 (CL0010) SH3_5; Bacterial SH3 domain (PF08460; HMM-score: 34.4)
  SH3_3; Bacterial SH3 domain (PF08239; HMM-score: 17)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009625
  • TAT(Tat/SPI): 0.000385
  • LIPO(Sec/SPII): 0.00085
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160059 NCBI
• RefSeq: YP_494080 NCBI
• UniProt: A0A0H2XDT4 UniProt

⊟Protein sequence

• MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMLATGERLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESANLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGNKAKGIIKQATTKKEAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLRHAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGESASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSELGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGVVASEVKAPVKNEKNPPVPAGYTLDKNNVPYKKEQGNYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRWITYIANSGQRRYIATGEVDIAGNRISSFGKFSAV

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1383 < SAUSA300_1384

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications