COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1410 [new locus tag: SAUSA300_RS07695 ]
• pan locus tag^?:
• symbol: SAUSA300_1410
• pan gene symbol^?: —
• synonym:
• product: virulence-associated protein E

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1410 [new locus tag: SAUSA300_RS07695 ]
• symbol: SAUSA300_1410
• product: virulence-associated protein E
• replicon: chromosome
• strand: -
• coordinates: 1575087..1577534
• length: 2448
• essential: unknown

⊟Accession numbers

• Gene ID: 3914804 NCBI
• RefSeq: YP_494107 NCBI
• BioCyc: see SAUSA300_RS07695
• MicrobesOnline: 1292925 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  1861
  1921
  1981
  2041
  2101
  2161
  2221
  2281
  2341
  2401
  
  ATGTTAGACAAAGTCACTCAAATAGAAACAATTAAATATGATCGTGATGTCTCATATTCT
  TATGCTGCTAGTCGTTTATCCACATATTGGACTAATCACAATATGGCTTGGTCTGACTTT
  ATGCAGAAGCTAGCACAAACAGTTAGAACTAAAGAAGATTTAACTGAGTACAATAAAATG
  TCTAAGTCTGAACAAGCAGATATAAAAGATGTTGGCGGATTTGTCGGTGGATATTTAAAA
  GAAGGCAAACGACGTGCTGGTCAAGTCATGAATCGTTCAATGCTAACACTTGATATCGAT
  TATGCTGCTCAAGATATGACTGACATATTATCTATGTTTTATGATTTTGCATATTGTTTA
  TATTCAACACATAAGCATAGAGAGATAAGTCCAAGACTGCGTTTAGTGATTCCTTTAAAA
  CGAAATGTAAATGCAGATGAGTATGAAGCTATTGGGCGTAAAGTCGCAGATATCGTTGGC
  ATGGATTACTTCGATGATACAACTTATCAACCACATAGGTTAATGTATTGGCCTTCAACT
  AGCAATGATGCAGAATTTTTCTTTACCTATGAAGATTTACCTTTGTTAGATCCAGATAAA
  ATATTAAATGAATATGTTGATTGGACTGACACATTAGAATGGCCAACGTCTTCAAAGGAA
  GAGAGTAAGACTAAAAGATTAGCAGATAAGCAAGGTGACCCAGAAGAAAAGCCGGGAATT
  GTTGGCGCATTTTGTAGAGCCTATACGATAGAAGAAGCTATATCAACTTTTATTCCTGAC
  TTATACGAAAAACATTCTACTAACCGTTATACCTATCATGAAGGTTCAACTGCAGGTGGA
  TTGGTGTTATACGAAAATAACAAGTTTGCCTATTCTCATCATAATACGGATCCCGTTAGC
  GGTATGCTTGTGAACAGTTTTGATTTAGTACGCATACACTTATATGGTGCTCAAGATGAA
  GACGCTAAAACAGATACTCCGGTTAATCGACTACCTAGTTATAAAGCAATGCAGCAAAGA
  GCGCAAAATGATGAAGTTGTTAAAAAGCAATTAATTAACGACAAAATGTCTGATGCAATG
  CAGGATTTCGATGAAATAGTAAATAGCGATGATGCATGGTCTGAGACGTTAGAAATTACT
  TCGAAAGGTACTTTCAAAGCTAGTATCCCAAATATAGAAATTATATTGCGTAATGATCCA
  AATTTAAAAGGAAAAATAGCATTTAATGAATTTACAAAACAAATTGAATGCTTAGGGAAA
  GTGCCATGGAATACTAATTTTAAGACACGTCAATGGCAAGACGGTGATGATAGCAGTTTA
  AGAAGTTATATCGAAAAGATTTATGACATACACCATTCAGGTAAAACAAAAGATGCCATT
  ATAAGCGTAGCAATGCAAAATGCTTATCATCCAGTAAGGGATTATCTAAATAAAATATCG
  TGGGATGGACATAAACGTCTTGAAAAGTTATTTATCAAATACTTAGGTGTTGAAGATACT
  GAAGTGAATAGAACAACTACCAAAAAAGCATTGACTGCTGGAATTGCTCGAGTAATGGAG
  CCTGGATGTAAATTTGACTATATGCTTACACTTTATGGTCCTCAAGGTGTAGGTAAATCT
  GCTTTGCTAAAAAAATTAGGTGGTGCATGGTTTTCTGACAGTTTAGTTTCTGTTACAGGT
  AAAGAAGCCTATGAGGCCTTACAAGGCGTTTGGCTAATGGAAATGGCAGAACTTGCAGCT
  ACAAGAAAAGCTGAAGTTGAAGCTATTAAGCATTTCATATCTAAACAAGTTGACCGGTTT
  CGTGTTGCTTATGGACATTATATTGAAGATTTTCCAAGGCAATGTATTTTCATTGGTACA
  ACTAATAAAGTTGATTTCTTAAGAGATGAAACTGGTGGAAGACGTTTTTGGCCAATGACT
  GTAAATCCAGAGAGAGTTGAAGTGAACTGGTCTAAACTAACCAAAGAAGAGATCGACCAA
  ATTTGGGCAGAAGCTAAATATTATTATGAACAAGGAGAAGAGTTATTCCTCAACCCTGAA
  CTAGAAGAAGAAATGCGTTCAATACAAAGCAAACATACTGAGGAATCTCCATATACAGGC
  ATTATTGATGAATATCTTAACACACCAATTCCTAGCAATTGGGATGACTTAACTATCTTT
  GAACGAAGACGATTTTATCAAGGTGATGTTGATATGTTACCAACAGGAAATGTAGATTAC
  GTTGAAAGAAATAAGGTCTGTGCGCTTGAAGTGTTTGTTGAATGTTTTGGTAAAGATAAG
  GGAGATAGTAGAGGATCTATGGAAATTAGAAAGATTTCAAACATCTTAAGACAATTAGAC
  AATTGGTCTGTATATGATGGTAATAAAAGTGGGAAAATTCGATTTGGAAAAGATTATGGT
  GTACAGATAGCTTATGTAAGAGATGAAAGTTTAGAAGATTTAATATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1740
  1800
  1860
  1920
  1980
  2040
  2100
  2160
  2220
  2280
  2340
  2400
  2448
  

⊟Protein

⊟General

• locus tag: SAUSA300_1410 [new locus tag: SAUSA300_RS07695 ]
• symbol: SAUSA300_1410
• description: virulence-associated protein E
• length: 815
• theoretical pI: 5.02446
• theoretical MW: 94187.4
• GRAVY: -0.61362

⊟Function

• TIGRFAM:
• TheSEED  :
  • DNA primase, phage associated
• PFAM:
  P-loop_NTPase (CL0023) VirE; Virulence-associated protein E (PF05272; HMM-score: 289.7)
  and 8 more
  AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 19.3)
  AAA_16; AAA ATPase domain (PF13191; HMM-score: 15.4)
  AAA_22; AAA domain (PF13401; HMM-score: 13)
  ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 12.8)
  NACHT; NACHT domain (PF05729; HMM-score: 12.1)
  RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 11.8)
  AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 11.8)
  RNA_helicase; RNA helicase (PF00910; HMM-score: 11.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010167
  • TAT(Tat/SPI): 0.000581
  • LIPO(Sec/SPII): 0.001343
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161308 NCBI
• RefSeq: YP_494107 NCBI
• UniProt: A0A0H2XII2 UniProt

⊟Protein sequence

• MLDKVTQIETIKYDRDVSYSYAASRLSTYWTNHNMAWSDFMQKLAQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGYLKEGKRRAGQVMNRSMLTLDIDYAAQDMTDILSMFYDFAYCLYSTHKHREISPRLRLVIPLKRNVNADEYEAIGRKVADIVGMDYFDDTTYQPHRLMYWPSTSNDAEFFFTYEDLPLLDPDKILNEYVDWTDTLEWPTSSKEESKTKRLADKQGDPEEKPGIVGAFCRAYTIEEAISTFIPDLYEKHSTNRYTYHEGSTAGGLVLYENNKFAYSHHNTDPVSGMLVNSFDLVRIHLYGAQDEDAKTDTPVNRLPSYKAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIVNSDDAWSETLEITSKGTFKASIPNIEIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTNFKTRQWQDGDDSSLRSYIEKIYDIHHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGHKRLEKLFIKYLGVEDTEVNRTTTKKALTAGIARVMEPGCKFDYMLTLYGPQGVGKSALLKKLGGAWFSDSLVSVTGKEAYEALQGVWLMEMAELAATRKAEVEAIKHFISKQVDRFRVAYGHYIEDFPRQCIFIGTTNKVDFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEIDQIWAEAKYYYEQGEELFLNPELEEEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWDDLTIFERRRFYQGDVDMLPTGNVDYVERNKVCALEVFVECFGKDKGDSRGSMEIRKISNILRQLDNWSVYDGNKSGKIRFGKDYGVQIAYVRDESLEDLI

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications