PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1430 [new locus tag: SAUSA300_RS07805 ]
• pan locus tag^?: SAUPAN001309000
• symbol: SAUSA300_1430
• pan gene symbol^?: —
• synonym:
• product: phiSLT ORF 87-like protein DNA-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1430 [new locus tag: SAUSA300_RS07805 ]
• symbol: SAUSA300_1430
• product: phiSLT ORF 87-like protein DNA-binding protein
• replicon: chromosome
• strand: -
• coordinates: 1586683..1586946
• length: 264
• essential: unknown

⊟Accession numbers

• Gene ID: 3913853 NCBI
• RefSeq: YP_494127 NCBI
• BioCyc: see SAUSA300_RS07805
• MicrobesOnline: 1292945 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  
  ATGCCAAAAATCATAGTACCACCAACACCAGAAAACACATATAGAGGCGAAGAAAAATTT
  GTGAAAAAGTTATACGCAACACCTACACAAATCCATCAATTGTTTGGAGTATGTAGAAGT
  ACAGTATACAACTGGTTGAAATATTACCGCAAAGATAATTTAGGTGTAGAAAATTTATAC
  ATTGATTATTCACCAACAGGCACTCTGATTAATATTTCTAAATTGGAAGAGTATTTGATC
  AGAAAGCATAAAAAATGGTATTAG
  60
  120
  180
  240
  264
  

⊟Protein

⊟General

• locus tag: SAUSA300_1430 [new locus tag: SAUSA300_RS07805 ]
• symbol: SAUSA300_1430
• description: phiSLT ORF 87-like protein DNA-binding protein
• length: 87
• theoretical pI: 9.82716
• theoretical MW: 10444.1
• GRAVY: -0.611494

⊟Function

• TIGRFAM:
• TheSEED  :
  • Phage protein
• PFAM:
  HTH (CL0123) HTH_23; Homeodomain-like domain (PF13384; HMM-score: 30.7)
  and 9 more
  HTH_29; Winged helix-turn helix (PF13551; HMM-score: 21)
  HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 20.2)
  Terminase_5; Putative ATPase subunit of terminase (gpP-like) (PF06056; HMM-score: 19)
  HTH_Tnp_IS630; Transposase (PF01710; HMM-score: 17)
  HTH_17; Helix-turn-helix domain (PF12728; HMM-score: 16.6)
  HTH_OrfB_IS605; Helix-turn-helix domain (PF12323; HMM-score: 16)
  no clan defined DUF6570; Domain of unknown function (DUF6570) (PF20209; HMM-score: 16)
  HTH (CL0123) CENP-B_N; CENP-B N-terminal DNA-binding domain (PF04218; HMM-score: 14.9)
  no clan defined Xre-MbcA-ParS_M; Antitoxin Xre/MbcA/ParS-like, middle domain (PF23125; HMM-score: 13.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9387
  • Cytoplasmic Membrane Score: 0.0138
  • Cell wall & surface Score: 0.0016
  • Extracellular Score: 0.046
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009984
  • TAT(Tat/SPI): 0.000407
  • LIPO(Sec/SPII): 0.001073
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161244 NCBI
• RefSeq: YP_494127 NCBI
• UniProt: A0A0H2XI64 UniProt

⊟Protein sequence

• MPKIIVPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYNWLKYYRKDNLGVENLYIDYSPTGTLINISKLEEYLIRKHKKWY

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1430 < SAUSA300_1431

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications