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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1498 [new locus tag: SAUSA300_RS08175 ]
  • pan locus tag?: SAUPAN004114000
  • symbol: gcvT
  • pan gene symbol?: gcvT
  • synonym:
  • product: glycine cleavage system aminomethyltransferase T

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1498 [new locus tag: SAUSA300_RS08175 ]
  • symbol: gcvT
  • product: glycine cleavage system aminomethyltransferase T
  • replicon: chromosome
  • strand: -
  • coordinates: 1651614..1652705
  • length: 1092
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGTCAAGTGATTTAAAACAAACACCTTTATATCAAAATTATGTTGATAGAGGTGCAAAA
    ATTGTGGAATTCGGAGGATGGGCGATGCCTGTTCAATTTTCAAGTATTAAAGAGGAGCAT
    AATGCTGTTCGATACGAAATTGGCCTGTTTGATGTTAGTCATATGGGTGAAATTGAAGTA
    ACAGGTAAAGATGCTAGTCAGTTTGTGCAATATTTATTATCAAATGATACTGATAATTTA
    ACTACTTCAAAAGCATTATATACTGCTTTATGTAATGAAGAAGGCGGTATTATTGATGAT
    TTAGTAATATATAAATTAGCTGACGACAATTATTTATTAGTTGTTAATGCTGCTAATACT
    GAAAAAGATTTTAATTGGATTTTAAAACACAAAGAGAAATTTGATGTTGAAGTACAAAAT
    GTATCAAACCAATATGGTCAATTAGCAATACAAGGACCAAAAGCTAGAGATTTAATTAAT
    CAATTAGTTGATGAAGATGTAACTGAAATGAAAATGTTTGAATTTAAACAGGGTGTCAAA
    TTATTTGGAGCAAACGTCATTTTATCTCAGTCAGGTTACACAGGTGAAGATGGGTTTGAA
    ATTTATTGTAATATCGATGATACTGAAAAAATTTGGGATGGTTTATTAGAGTATAATGTT
    ATGCCATGTGGGTTAGGCGCTCGTGATACATTAAGATTAGAGGCTGGATTGCCATTACAT
    GGACAAGATTTAACTGAATCAATTACACCATATGAAGGTGGTATCGCTTTTGCAAGTAAA
    CCATTAATTGATGCTGATTTTATTGGTAAATCTGTATTAAAAGATCAAAAAGAAAATGGT
    GCACCAAGAAGAACAGTGGGATTAGAATTACTTGAAAAAGGAATTGCAAGAACTGGTTAT
    GAAGTTATGGATTTAGATGGAAATATTATTGGAGAAGTAACTTCAGGAACACAGTCTCCA
    TCATCAGGAAAATCAATTGCACTTGCAATGATAAAAAGAGATGAGTTTGAAATGGGTAGA
    GAGTTGCTTGTTCAAGTTCGTAAGCGTCAATTAAAAGCGAAAATTGTTAAGAAAAATCAA
    ATTGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1092

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1498 [new locus tag: SAUSA300_RS08175 ]
  • symbol: GcvT
  • description: glycine cleavage system aminomethyltransferase T
  • length: 363
  • theoretical pI: 4.54011
  • theoretical MW: 40457.6
  • GRAVY: -0.331956

Function[edit | edit source]

  • reaction:
    EC 2.1.2.10?  ExPASy
    Aminomethyltransferase [Protein]-S8-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3
  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines glycine cleavage system T protein (TIGR00528; EC 2.1.2.10; HMM-score: 442)
    and 2 more
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 187.7)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, gamma subunit family (TIGR01375; EC 1.5.3.1; HMM-score: 22)
  • TheSEED:  
    Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10)
    and 1 more
    Glycine cleavage system  Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10)
  • PFAM:
    FBD (CL0289) GCV_T; Aminomethyltransferase folate-binding domain (PF01571; HMM-score: 318.2)
    and 3 more
    no clan defined GCV_T_C; Glycine cleavage T-protein C-terminal barrel domain (PF08669; HMM-score: 86.6)
    FBD (CL0289) SoxG; Sarcosine oxidase, gamma subunit family (PF04268; HMM-score: 24.3)
    no clan defined DUF3659; Protein of unknown function (DUF3659) (PF12396; HMM-score: 13)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.0045
    • TAT(Tat/SPI): 0.000579
    • LIPO(Sec/SPII): 0.000703
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVRYEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLVIYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLINQLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEYNVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQKENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRDEFEMGRELLVQVRKRQLKAKIVKKNQIDK

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: Gly-box (transcription antitermination) regulon
    Gly-box(RNA)important in Glycine metabolism; regulatory site identified based on RegPrecise data for N315 RegPrecise  

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]