PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1498 [new locus tag: SAUSA300_RS08175 ]
• pan locus tag^?: SAUPAN004114000
• symbol: gcvT
• pan gene symbol^?: gcvT
• synonym:
• product: glycine cleavage system aminomethyltransferase T

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1498 [new locus tag: SAUSA300_RS08175 ]
• symbol: gcvT
• product: glycine cleavage system aminomethyltransferase T
• replicon: chromosome
• strand: -
• coordinates: 1651614..1652705
• length: 1092
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913255 NCBI
• RefSeq: YP_494193 NCBI
• BioCyc: see SAUSA300_RS08175
• MicrobesOnline: 1293013 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGTCAAGTGATTTAAAACAAACACCTTTATATCAAAATTATGTTGATAGAGGTGCAAAA
  ATTGTGGAATTCGGAGGATGGGCGATGCCTGTTCAATTTTCAAGTATTAAAGAGGAGCAT
  AATGCTGTTCGATACGAAATTGGCCTGTTTGATGTTAGTCATATGGGTGAAATTGAAGTA
  ACAGGTAAAGATGCTAGTCAGTTTGTGCAATATTTATTATCAAATGATACTGATAATTTA
  ACTACTTCAAAAGCATTATATACTGCTTTATGTAATGAAGAAGGCGGTATTATTGATGAT
  TTAGTAATATATAAATTAGCTGACGACAATTATTTATTAGTTGTTAATGCTGCTAATACT
  GAAAAAGATTTTAATTGGATTTTAAAACACAAAGAGAAATTTGATGTTGAAGTACAAAAT
  GTATCAAACCAATATGGTCAATTAGCAATACAAGGACCAAAAGCTAGAGATTTAATTAAT
  CAATTAGTTGATGAAGATGTAACTGAAATGAAAATGTTTGAATTTAAACAGGGTGTCAAA
  TTATTTGGAGCAAACGTCATTTTATCTCAGTCAGGTTACACAGGTGAAGATGGGTTTGAA
  ATTTATTGTAATATCGATGATACTGAAAAAATTTGGGATGGTTTATTAGAGTATAATGTT
  ATGCCATGTGGGTTAGGCGCTCGTGATACATTAAGATTAGAGGCTGGATTGCCATTACAT
  GGACAAGATTTAACTGAATCAATTACACCATATGAAGGTGGTATCGCTTTTGCAAGTAAA
  CCATTAATTGATGCTGATTTTATTGGTAAATCTGTATTAAAAGATCAAAAAGAAAATGGT
  GCACCAAGAAGAACAGTGGGATTAGAATTACTTGAAAAAGGAATTGCAAGAACTGGTTAT
  GAAGTTATGGATTTAGATGGAAATATTATTGGAGAAGTAACTTCAGGAACACAGTCTCCA
  TCATCAGGAAAATCAATTGCACTTGCAATGATAAAAAGAGATGAGTTTGAAATGGGTAGA
  GAGTTGCTTGTTCAAGTTCGTAAGCGTCAATTAAAAGCGAAAATTGTTAAGAAAAATCAA
  ATTGATAAATAA
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  1092
  

⊟Protein

⊟General

• locus tag: SAUSA300_1498 [new locus tag: SAUSA300_RS08175 ]
• symbol: GcvT
• description: glycine cleavage system aminomethyltransferase T
• length: 363
• theoretical pI: 4.54011
• theoretical MW: 40457.6
• GRAVY: -0.331956

⊟Function

• reaction:
  EC 2.1.2.10?  ExPASy
  Aminomethyltransferase [Protein]-S⁸-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH₃
• TIGRFAM:
  Metabolism Energy metabolism Amino acids and amines glycine cleavage system T protein (TIGR00528; EC 2.1.2.10; HMM-score: 442)
  and 2 more
  Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 187.7)
  Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, gamma subunit family (TIGR01375; EC 1.5.3.1; HMM-score: 22)
• TheSEED  :
  • Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10)
  Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10)
  and 1 more
  Amino Acids and Derivatives Alanine, serine, and glycine Glycine cleavage system  Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10)
• PFAM:
  FBD (CL0289) GCV_T; Aminomethyltransferase folate-binding domain (PF01571; HMM-score: 318.2)
  and 3 more
  no clan defined GCV_T_C; Glycine cleavage T-protein C-terminal barrel domain (PF08669; HMM-score: 86.6)
  FBD (CL0289) SoxG; Sarcosine oxidase, gamma subunit family (PF04268; HMM-score: 24.3)
  no clan defined DUF3659; Protein of unknown function (DUF3659) (PF12396; HMM-score: 13)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.0045
  • TAT(Tat/SPI): 0.000579
  • LIPO(Sec/SPII): 0.000703
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160187 NCBI
• RefSeq: YP_494193 NCBI
• UniProt: Q2FGI5 UniProt

⊟Protein sequence

• MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVRYEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLVIYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLINQLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEYNVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQKENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRDEFEMGRELLVQVRKRQLKAKIVKKNQIDK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1496 < SAUSA300_1497 < gcvT

⊟Regulation

• regulator: Gly-box (transcription antitermination) regulon

  Gly-box

  (RNA)

  important in Glycine metabolism; regulatory site identified based on RegPrecise data for N315 RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications