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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1520 [new locus tag: SAUSA300_RS08295 ]
  • pan locus tag?: SAUPAN004141000
  • symbol: SAUSA300_1520
  • pan gene symbol?: trmK
  • synonym:
  • product: hypothetical protein

trmK: tRNA m1A22 methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1520 [new locus tag: SAUSA300_RS08295 ]
  • symbol: SAUSA300_1520
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1670488..1671165
  • length: 678
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGATTTCGTTAAATAACCGATTAACGACGGTAAGTCGATTTTTAAAACAAGGTACAATC
    GCGGATATTGGCTCAGATCATGCTTATTTGCCAATCTATGCAATTCAAAACCATTTATGC
    GAATGCGGTATTGCTGGAGAAGTTATTCAAGGCCCTTTTCAAGCTGCTGTGAAAAATGTA
    GCTGCAAACCAATTAGTAGATAGAATTGATGTACGTTTAGGGGATGGCTTAAGTGTTATT
    CAACCTGAGGACGTAATCGATAATATCACAATTTGTGGTATGGGCGGACCACTTATTGCT
    AAAATTTTAAAAGATGGGCAAGATAAGTTAAGTCAACATCCAAGGTTGATTTTACAAAGT
    AATATCCAAACTGAAAATTTAAGACAAACATTACAACAATTGAATTATGAAATAATTGAT
    GAAATAATAATGGAAGAAAAAGGCCATATTTATGAAATTGTTGTAGCAGAATATTCTACA
    GAATTAATAGAGCTATCATCTGATGAATTGAAATTCGGACCAAAATTGCTGAACAACAAA
    AATGAATATTTTATAAAAAAATGGCAAAGAGAGTTAGAAGCACTTTATCATATTAAATCG
    AAACTCAATACTGAACAACATCATCAACGTTTAGCTCAAATTAATGACGAAATTGCTGTA
    ATTGAAAGGGTGTTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    678

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1520 [new locus tag: SAUSA300_RS08295 ]
  • symbol: SAUSA300_1520
  • description: hypothetical protein
  • length: 225
  • theoretical pI: 5.13535
  • theoretical MW: 25508.1
  • GRAVY: -0.204444

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Putative tRNA-m1A22 methylase
  • PFAM:
    NADP_Rossmann (CL0063) TrmK; tRNA (adenine(22)-N(1))-methyltransferase (PF04816; HMM-score: 270.8)
    and 1 more
    Methyltransf_18; Methyltransferase domain (PF12847; HMM-score: 165)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.021876
    • TAT(Tat/SPI): 0.001395
    • LIPO(Sec/SPII): 0.000849
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGIAGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKILKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYSTELIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEIAVIERVL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Pamela Sweeney, Ashleigh Galliford, Abhishek Kumar, Dinesh Raju, Naveen B Krishna, Emmajay Sutherland, Caitlin J Leo, Gemma Fisher, Roopa Lalitha, Likith Muthuraj, Gladstone Sigamani, Verena Oehler, Silvia Synowsky, Sally L Shirran, Tracey M Gloster, Clarissa M Czekster, Pravin Kumar, Rafael G da Silva
    Structure, dynamics, and molecular inhibition of the Staphylococcus aureus m 1A22-tRNA methyltransferase TrmK.
    J Biol Chem: 2022, 298(6);102040
    [PubMed:35595101] [WorldCat.org] [DOI] (I p)
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