PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1542 [new locus tag: SAUSA300_RS08405 ]
• pan locus tag^?: SAUPAN004165000
• symbol: hrcA
• pan gene symbol^?: hrcA
• synonym:
• product: heat-inducible transcription repressor HrcA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1542 [new locus tag: SAUSA300_RS08405 ]
• symbol: hrcA
• product: heat-inducible transcription repressor HrcA
• replicon: chromosome
• strand: -
• coordinates: 1691970..1692947
• length: 978
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913931 NCBI
• RefSeq: YP_494237 NCBI
• BioCyc: see SAUSA300_RS08405
• MicrobesOnline: 1293057 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  961
  
  ATGATTACAGATAGGCAATTGAGTATATTAAACGCAATTGTTGAGGATTATGTTGATTTT
  GGACAACCCGTTGGTTCTAAAACACTAATTGAGCGACATAACTTGAATGTTAGTCCTGCT
  ACAATTAGAAATGAGATGAAACAGCTTGAAGATTTAAACTATATCGAGAAGACACATAGT
  TCTTCAGGGCGTTCGCCATCACAATTAGGTTTTAGGTATTATGTCAATCGTTTACTTGAA
  CAAACATCTCATCAAAAAACAAATAAATTAAGACGATTAAATCAATTGTTAGTTGAGAAT
  CAATATGATGTATCATCAGCATTGACATATTTTGCAGATGAATTATCAAATATATCTCAA
  TATACAACTTTAGTTGTTCATCCTAATCATAAACAAGATATTATCAATAATGTACACTTG
  ATTCGTGCTAATCCTAATTTAGTTATAATGGTTATCGTATTTTCATCAGGTCATGTTGAG
  CATGTACATCTTGCTTCAGACATACCGTTTAATAATGACAAACTAAATACAATATCAAAT
  TTTGTTACAAACAAACTAACTGAGTTTAATCAGAATTTACAAGATGATATTGTCTCTTTT
  GTTCAATCAGAGCAGGAAGAAATTTTTATCAATAAATTGTTAAATACGATGAATAATCAT
  ATTTCTAATCAAAGTAATAGCATTTATATGGGTGGAAAGGTTAAACTTATTGATGCATTG
  AATGAAAGTAATGTATCTTCAATTCAGCCCATTCTACAATATATAGAATCAAATAGAATT
  GCAGAATTATTACAAGATATATCTTCACCAAATATTAATGTAAAAATTGGTAATGAAATT
  GATGACAGCTTAAGTGATATTTCAATTGTAACAAGTCAATATCATTTTGATGAAACGTTA
  AAAGGTCAAATCGCTGTGATTGGACCTACAGCTATGCATTATCAAAATGTCATTCAATTA
  TTAAATAGAATTTGGTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1542 [new locus tag: SAUSA300_RS08405 ]
• symbol: HrcA
• description: heat-inducible transcription repressor HrcA
• length: 325
• theoretical pI: 5.71442
• theoretical MW: 37015.4
• GRAVY: -0.301231

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions heat-inducible transcription repressor HrcA (TIGR00331; HMM-score: 345.7)
• TheSEED  :
  • Heat-inducible transcription repressor HrcA
  Stress Response Heat shock Heat shock dnaK gene cluster extended  Heat-inducible transcription repressor HrcA
• PFAM:
  GAF (CL0161) HrcA; HrcA protein C terminal domain (PF01628; HMM-score: 138.5)
  and 8 more
  HTH (CL0123) HrcA_DNA-bdg; Winged helix-turn-helix transcription repressor, HrcA DNA-binding (PF03444; HMM-score: 18.7)
  HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 18.2)
  HTH_11; HTH domain (PF08279; HMM-score: 16.7)
  no clan defined FOXO_KIX_bdg; KIX-binding domain of forkhead box O, CR2 (PF16675; HMM-score: 13.3)
  HTH (CL0123) HTH_34; Winged helix DNA-binding domain (PF13601; HMM-score: 12.5)
  LexA_DNA_bind; LexA DNA binding domain (PF01726; HMM-score: 12.4)
  Tubulin_C (CL0442) FtsZ_C; FtsZ family, C-terminal domain (PF12327; HMM-score: 12.3)
  HTH (CL0123) Ribosomal_S19e; Ribosomal protein S19e (PF01090; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• stimulus: Heat shock
• genes regulated by HrcA, TF important in Heat shock responseRegPrecise

  repression

  dnaJ < dnaK < grpE < hrcA

  groEL < groES

  transcription units transferred from N315 data RegPrecise

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004618
  • TAT(Tat/SPI): 0.002068
  • LIPO(Sec/SPII): 0.000521
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161015 NCBI
• RefSeq: YP_494237 NCBI
• UniProt: Q2FGE1 UniProt

⊟Protein sequence

• MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRNEMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQYDVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVEHVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMNNHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKIGNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1536 < SAUSA300_1537 < prmA < dnaJ < dnaK < grpE < hrcA
  SAUSA300_1536 < SAUSA300_1537 < prmA < dnaJ < dnaK < grpE < hrcA  

⊟Regulation

• regulator: HrcA (repression) regulon

  HrcA

  (TF)

  important in Heat shock response; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

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⊟Literature

⊟References

⊟Relevant publications