PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1648 [new locus tag: SAUSA300_RS08995 ]
• pan locus tag^?: SAUPAN004349000
• symbol: SAUSA300_1648
• pan gene symbol^?: —
• synonym:
• product: putative NADP-dependent malic enzyme

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1648 [new locus tag: SAUSA300_RS08995 ]
• symbol: SAUSA300_1648
• product: putative NADP-dependent malic enzyme
• replicon: chromosome
• strand: -
• coordinates: 1809775..1811004
• length: 1230
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915297 NCBI
• RefSeq: YP_494342 NCBI
• BioCyc: see SAUSA300_RS08995
• MicrobesOnline: 1293163 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGTCATTAAGAGATGAAGCATTGGAAATGCACAAACGTAATCAAGGTAAATTAGAAGTT
  AAACCAAATGTAAAAGTTACTAATAAAGAGGAATTAAGTTTAGCATACTCACCTGGCGTT
  GCTGAACCGTGTAAAGATATTTATGAAGATAAAAGAAAAGTATATGATTACACAATTAAA
  GGAAATACAGTTGCAGTTATTACTGATGGAACAGCGGTATTAGGTTTAGGTAACATTGGA
  CCTGAAGCAAGTATTCCTGTAATGGAAGGTAAAGCAGTATTATTCAAAAGCTTCGCTGGT
  ATCAATGGGGTGCCTATTGCGTTAAATACAACTGATACCGAAGAAATCATTAAAACAGTT
  AAGTTGTTAGAACCTAATTATGGTGGTATTAATTTAGAGGATATTTCGGCACCACGTTGT
  TTTGAAATTGAAGAACGATTGAAAAAAGAAACTAATATTCCGGTATTCCATGACGATCAA
  CATGGTACAGCAATTGTAACATTGGCAGGTTTGGTAAATGCATTGAGAGTTGTTAACAAA
  GATATTGCTAAAATAAAAGTTGTACTAAATGGTGCTGGTGCAGCAGGAATAGCCATTGTT
  AAATTACTATACGCGTATGGTGTAAGAAATATGGTTATGTGTGACTCAAGAGGCGCAATT
  TTTGAAGGACGTTCATATGGTATGAATCCTACGAAAGATGTTGTAGCAAAATGGACAAAT
  AAAGATAAGATTGAAGGGTCTTTAGAAGAAGTCGTAAAAGATGCAGATGTATTTATCGGG
  GTTTCTGTAGCTAATGCGCTGTCACAAGATATGGTTAAGAGTATGGCAGATAATCCAATT
  ATATTTGCAATGGCTAATCCAAATCCTGAAATAATACCTGATGATGCCAAAGCGGCAGGT
  GCACGAGTTGTTGGTACAGGACGTTCAGACTATCCTAACCAAATTAATAATGTATTAGCT
  TTCCCTGGTATTTTTAGAGGTGCATTAGAGGTTGAAGCTACACATATAAATGAAGAAATG
  AAAAAGGCAGCTGTAGAAGCGATTGCTGATTTAATCGATAGTTCTGAATTAAATGAAGAC
  TACTGTATCCCAGGACCGTTTGATAAACGTGTAGCGCCATCAGTTGCTCGTAATGTTGCT
  AAAGCGGCAATGGAATCTGGAGTAGCTAGGATTGAAGTTGATCCGCAAGATGTGTATGAT
  AAAACAATGAAACTTACAGATTTACAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1648 [new locus tag: SAUSA300_RS08995 ]
• symbol: SAUSA300_1648
• description: putative NADP-dependent malic enzyme
• length: 409
• theoretical pI: 4.81628
• theoretical MW: 44233.4
• GRAVY: -0.122738

⊟Function

• TIGRFAM:
  E1-like protein-activating enzyme Gsa7p/Apg7p (TIGR01381; HMM-score: 13.3)
• TheSEED  :
  • NADP-dependent malic enzyme (EC 1.1.1.40)
  Carbohydrates Central carbohydrate metabolism Pyruvate metabolism I: anaplerotic reactions, PEP  NADP-dependent malic enzyme (EC 1.1.1.40)
• PFAM:
  AA_dh_N (CL0603) malic; Malic enzyme, N-terminal domain (PF00390; HMM-score: 109.6)
  NADP_Rossmann (CL0063) Malic_M; Malic enzyme, NAD binding domain (PF03949; HMM-score: 92.5)
  and 6 more
  ELFV_dehydrog; Glutamate/Leucine/Phenylalanine/Valine dehydrogenase (PF00208; HMM-score: 15.4)
  ThiF; ThiF family (PF00899; HMM-score: 15.1)
  DapB_N; Dihydrodipicolinate reductase, N-terminus (PF01113; HMM-score: 13.2)
  no clan defined Gly_rich_SFCGS; Glycine-rich SFCGS (PF14272; HMM-score: 13.2)
  NADP_Rossmann (CL0063) FAD_binding_3; FAD binding domain (PF01494; HMM-score: 12.6)
  Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 9.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mg²⁺, Mn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.012323
  • TAT(Tat/SPI): 0.003306
  • LIPO(Sec/SPII): 0.001611
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161326 NCBI
• RefSeq: YP_494342 NCBI
• UniProt: A0A0H2XH21 UniProt

⊟Protein sequence

• MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPCKDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGINGVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQHGTAIVTLAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRGAIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMADNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEATHINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIEVDPQDVYDKTMKLTDLQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

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⊟Literature

⊟References

⊟Relevant publications