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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1648 [new locus tag: SAUSA300_RS08995 ]
  • pan locus tag?: SAUPAN004349000
  • symbol: SAUSA300_1648
  • pan gene symbol?:
  • synonym:
  • product: putative NADP-dependent malic enzyme

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1648 [new locus tag: SAUSA300_RS08995 ]
  • symbol: SAUSA300_1648
  • product: putative NADP-dependent malic enzyme
  • replicon: chromosome
  • strand: -
  • coordinates: 1809775..1811004
  • length: 1230
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTCATTAAGAGATGAAGCATTGGAAATGCACAAACGTAATCAAGGTAAATTAGAAGTT
    AAACCAAATGTAAAAGTTACTAATAAAGAGGAATTAAGTTTAGCATACTCACCTGGCGTT
    GCTGAACCGTGTAAAGATATTTATGAAGATAAAAGAAAAGTATATGATTACACAATTAAA
    GGAAATACAGTTGCAGTTATTACTGATGGAACAGCGGTATTAGGTTTAGGTAACATTGGA
    CCTGAAGCAAGTATTCCTGTAATGGAAGGTAAAGCAGTATTATTCAAAAGCTTCGCTGGT
    ATCAATGGGGTGCCTATTGCGTTAAATACAACTGATACCGAAGAAATCATTAAAACAGTT
    AAGTTGTTAGAACCTAATTATGGTGGTATTAATTTAGAGGATATTTCGGCACCACGTTGT
    TTTGAAATTGAAGAACGATTGAAAAAAGAAACTAATATTCCGGTATTCCATGACGATCAA
    CATGGTACAGCAATTGTAACATTGGCAGGTTTGGTAAATGCATTGAGAGTTGTTAACAAA
    GATATTGCTAAAATAAAAGTTGTACTAAATGGTGCTGGTGCAGCAGGAATAGCCATTGTT
    AAATTACTATACGCGTATGGTGTAAGAAATATGGTTATGTGTGACTCAAGAGGCGCAATT
    TTTGAAGGACGTTCATATGGTATGAATCCTACGAAAGATGTTGTAGCAAAATGGACAAAT
    AAAGATAAGATTGAAGGGTCTTTAGAAGAAGTCGTAAAAGATGCAGATGTATTTATCGGG
    GTTTCTGTAGCTAATGCGCTGTCACAAGATATGGTTAAGAGTATGGCAGATAATCCAATT
    ATATTTGCAATGGCTAATCCAAATCCTGAAATAATACCTGATGATGCCAAAGCGGCAGGT
    GCACGAGTTGTTGGTACAGGACGTTCAGACTATCCTAACCAAATTAATAATGTATTAGCT
    TTCCCTGGTATTTTTAGAGGTGCATTAGAGGTTGAAGCTACACATATAAATGAAGAAATG
    AAAAAGGCAGCTGTAGAAGCGATTGCTGATTTAATCGATAGTTCTGAATTAAATGAAGAC
    TACTGTATCCCAGGACCGTTTGATAAACGTGTAGCGCCATCAGTTGCTCGTAATGTTGCT
    AAAGCGGCAATGGAATCTGGAGTAGCTAGGATTGAAGTTGATCCGCAAGATGTGTATGAT
    AAAACAATGAAACTTACAGATTTACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1230

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1648 [new locus tag: SAUSA300_RS08995 ]
  • symbol: SAUSA300_1648
  • description: putative NADP-dependent malic enzyme
  • length: 409
  • theoretical pI: 4.81628
  • theoretical MW: 44233.4
  • GRAVY: -0.122738

Function[edit | edit source]

  • TIGRFAM:
    E1-like protein-activating enzyme Gsa7p/Apg7p (TIGR01381; HMM-score: 13.3)
  • TheSEED  :
    • NADP-dependent malic enzyme (EC 1.1.1.40)
    Carbohydrates Central carbohydrate metabolism Pyruvate metabolism I: anaplerotic reactions, PEP  NADP-dependent malic enzyme (EC 1.1.1.40)
  • PFAM:
    AA_dh_N (CL0603) malic; Malic enzyme, N-terminal domain (PF00390; HMM-score: 109.6)
    NADP_Rossmann (CL0063) Malic_M; Malic enzyme, NAD binding domain (PF03949; HMM-score: 92.5)
    and 6 more
    ELFV_dehydrog; Glutamate/Leucine/Phenylalanine/Valine dehydrogenase (PF00208; HMM-score: 15.4)
    ThiF; ThiF family (PF00899; HMM-score: 15.1)
    DapB_N; Dihydrodipicolinate reductase, N-terminus (PF01113; HMM-score: 13.2)
    no clan defined Gly_rich_SFCGS; Glycine-rich SFCGS (PF14272; HMM-score: 13.2)
    NADP_Rossmann (CL0063) FAD_binding_3; FAD binding domain (PF01494; HMM-score: 12.6)
    Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 9.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+, Mn2+
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012323
    • TAT(Tat/SPI): 0.003306
    • LIPO(Sec/SPII): 0.001611
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPCKDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGINGVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQHGTAIVTLAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRGAIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMADNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEATHINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIEVDPQDVYDKTMKLTDLQ

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]