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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1658 [new locus tag: SAUSA300_RS09050 ]
  • pan locus tag?: SAUPAN004364000
  • symbol: SAUSA300_1658
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1658 [new locus tag: SAUSA300_RS09050 ]
  • symbol: SAUSA300_1658
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1823625..1824572
  • length: 948
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCAGAACAACAAACAATTATGGAACGCTTGTTTCATACATTAGATGAAAAAGCTAAA
    ACATTAAATAATGAAAATGGCCAAAGTTTTATTGAAAATCTTGGGCTAGCAATGGAACAA
    GTATATACCAATGAAAGAGGATTGTTAGAACAATCGACGTTACAAGATCGACGTAAAGCA
    TTCCAATTTGCATATTTAAGTTTAATGCAGGAAGAAAAGATACAAGCAAATCATCAAATT
    ACACCAGATTCAATTGGATTGATACTAGGATTTTTAGTTGAGCGTTTTATGAACAACCAA
    GAAGAATTACATATTGTTGATATTGCAAGTGGTGCCGGTCATTTAAGTGCTACTGTAAAA
    GAAGTGTTACCTGAAATTGCGGTTATGCATCATTTAATTGAAGTTGATCCAGTTTTATCA
    CGTGTTAGTGTACATTTAGCAAACTTCTTAGAAATTCCTTTCGATGTGTATCCTCAAGAT
    GCCATCATGCCACTACCATTAGAAGAAGCAGATATCGTTATTGGTGATTTTCCAGTAGGC
    TATTATCCAATTGATGAAAGAAGTAAGGAGTTTAAGCTAGGTTTTGAAGAAGGACATAGT
    TATTCACATTATTTATTAATAGAACAAGCAATAAATGCATTAAAAGATGCTGGATATGCC
    TTTCTAGTGGTACCAAGTAATATTTTTACAGGTGAACATGTAAAACAGCTTGAAAAATAT
    ATTGCAACAGAGACAGAGATGCAAGCATTTTTAAATTTACCACCAACTTTATTTAAAAAT
    GAAAAAGCGCGAAAATCTATATTAATTTTACAAAAGAAAAAATCGGGTGAAACAAAGCCA
    GTTGAAGTATTATTGGCAAATATTCCTGATTTCAAAATTCCTTCACAATTTCAAGGATTT
    ATGACAGAGTTAAATCAGTGGATGGACACAAATCGTCCTAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    948

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1658 [new locus tag: SAUSA300_RS09050 ]
  • symbol: SAUSA300_1658
  • description: hypothetical protein
  • length: 315
  • theoretical pI: 4.84817
  • theoretical MW: 35947
  • GRAVY: -0.233016

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism Restriction/modification type I restriction-modification system, M subunit (TIGR00497; EC 2.1.1.72; HMM-score: 14.5)
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 11.8)
  • TheSEED  :
    • Adenine-specific methyltransferase (EC 2.1.1.72)
    CBSS-257314.1.peg.752  Adenine-specific methyltransferase (EC 2.1.1.72)
  • PFAM:
    NADP_Rossmann (CL0063) N6_Mtase; N-6 DNA Methylase (PF02384; HMM-score: 38.4)
    and 3 more
    MTS; Methyltransferase small domain (PF05175; HMM-score: 22.2)
    RrnaAD; Ribosomal RNA adenine dimethylase (PF00398; HMM-score: 18.6)
    Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 13.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.0029
    • TAT(Tat/SPI): 0.00045
    • LIPO(Sec/SPII): 0.000312
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTNERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEELHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQDAIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAGYAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSGETKPVEVLLANIPDFKIPSQFQGFMTELNQWMDTNRPKK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]