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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1731 [new locus tag: SAUSA300_RS09470 ]
  • pan locus tag?: SAUPAN004503000
  • symbol: pckA
  • pan gene symbol?: pckA
  • synonym:
  • product: phosphoenolpyruvate carboxykinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1731 [new locus tag: SAUSA300_RS09470 ]
  • symbol: pckA
  • product: phosphoenolpyruvate carboxykinase
  • replicon: chromosome
  • strand: +
  • coordinates: 1915442..1917034
  • length: 1593
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCAGTAGACACATACACTGAAACAACTAAAATTGACAAATTACTGAAAAAACCAACG
    TCACATTTTCAACTTTCGACGACACAACTTTATAATAAAATCTTAGACAATAACGAAGGG
    GTATTAACAGAACTTGGTGCTGTTAATGCAAGTACTGGAAAATATACTGGTCGTTCGCCT
    AAAGACAAATTTTTTGTCTCTGAACCTTCATATAGAGATAACATTGATTGGGGAGAAATT
    AATCAACCTATCGATGAAGAAACTTTCTTGAAGTTATACCATAAAGTACTAGACTATTTA
    GATAAAAAAGATGAACTATACGTATTTAAAGGCTACGCTGGTAGCGATAAAGATACAATG
    TTAAAACTTACAGTCATCAATGAATTAGCATGGCATAATTTATTTGCTAAAAATATGTTT
    ATTAGACCTGAATCAAAAGAAGAAGCTACAAAGATTAAACCTAACTTCACTATCGTTTCT
    GCACCACATTTTAAAGCAGATCCAGAAGTTGATGGTACTAAATCTGAAACCTTTGTCATT
    ATTTCATTTAAACACAAAGTCATTTTAATCGGCGGTACTGAATACGCTGGTGAAATGAAA
    AAAGGTATCTTCTCTGTAATGAATTATCTCTTACCGATGCAAGATATTATGAGCATGCAT
    TGCTCAGCAAACGTTGGTGAAAAAGGCGATGTTGCATTATTCTTTGGTCTATCTGGCACT
    GGTAAAACAACCTTATCGGCTGACCCACACCGTAAACTAATCGGTGATGATGAACACGGC
    TGGAATAAAAACGGGGTCTTTAATATCGAAGGTGGCTGCTATGCAAAAGCAATTAATCTT
    TCCAAAGAAAAAGAACCACAGATTTTTGACGCAATCAAATATGGTGCAATTTTAGAGAAC
    ACTGTAGTTGCAGAAGATGGTTCAGTGGACTTTGAAGACAATCGTTATACAGAAAACACG
    CGTGCCGCTTATCCAATTAATCACATTGACAATATTGTAGTACCATCTAAAGCAGCACAT
    CCAAATACAATTATTTTCTTAACTGCGGATGCATTTGGTGTTATTCCACCGATTTCAAAG
    TTAAATAAAGACCAAGCAATGTATCATTTCTTGAGTGGTTTCACTTCTAAATTAGCTGGT
    ACAGAGCGTGGTGTGACAGAACCTGAACCATCATTCTCAACATGTTTCGGAGCACCGTTC
    TTCCCGTTACACCCTACTGTTTACGCTGATCTATTAGGTGAACTTATCGATTTACATGAT
    GTTGATGTTTATCTTGTTAATACTGGATGGACTGGCGGAAAATATGGTGTAGGACGTAGA
    ATCAGCTTACATTACACACGTCAAATGGTAAACCAAGCGATTTCTGGCAAATTGAAAAAT
    GCAGAATATACAAAAGATAGTACGTTTGGTTTAAGCATTCCTGTAGAAATTGAAGATGTA
    CCGAAAACAATTTTAAATCCAATTAATGCTTGGAGCGACAAAGAGAAATATAAAGCACAA
    GCAGAAGATTTAATTCAACGTTTTGAAAAGAACTTCGAAAAATTTGGTGAAAAAGTTGAA
    CATATTGCTGAAAAAGGTAGCTTCAACAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1593

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1731 [new locus tag: SAUSA300_RS09470 ]
  • symbol: PckA
  • description: phosphoenolpyruvate carboxykinase
  • length: 530
  • theoretical pI: 6.01708
  • theoretical MW: 59376.9
  • GRAVY: -0.388491

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.1.49?  ExPASy
    Phosphoenolpyruvate carboxykinase (ATP) ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2
  • TIGRFAM:
    Metabolism Energy metabolism Glycolysis/gluconeogenesis phosphoenolpyruvate carboxykinase (ATP) (TIGR00224; EC 4.1.1.49; HMM-score: 792.5)
    and 1 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 11.4)
  • TheSEED  :
    • Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49)
    Carbohydrates Central carbohydrate metabolism Pyruvate metabolism I: anaplerotic reactions, PEP  Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49)
    and 1 more
    Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49)
  • PFAM:
    PEP-carboxyk (CL0374) PEPCK_ATP; Phosphoenolpyruvate carboxykinase (PF01293; HMM-score: 735)
    and 2 more
    PEPCK_C; Phosphoenolpyruvate carboxykinase C-terminal P-loop domain (PF00821; HMM-score: 15.3)
    P-loop_NTPase (CL0023) AAA_33; AAA domain (PF13671; HMM-score: 13.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016392
    • TAT(Tat/SPI): 0.000978
    • LIPO(Sec/SPII): 0.000732
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTELGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDKKDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVSAPHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMSMHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAKAINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAEKGSFNK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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