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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1996 [new locus tag: SAUSA300_RS10975 ]
  • pan locus tag?: SAUPAN005287000
  • symbol: amt
  • pan gene symbol?: nrgA
  • synonym:
  • product: ammonium transporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1996 [new locus tag: SAUSA300_RS10975 ]
  • symbol: amt
  • product: ammonium transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2153789..2155039
  • length: 1251
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATCTTAACGATACGATATTTATGTTTTTGTGTACATTATTAGTTTGGTTAATGACA
    CCAGGATTAAGTTTATTTTATGGTGGGTTAGTTCAATCTAAAAATGCGCTTAATACTGTC
    ATGCAAAGTATGGCAGCAATTGTGCTTGTTACATTTGTATGGATAACAGTTGGTTTTACA
    ATTAGTTTTGGGAATGGGAATTTATGGTTCGGAAATTGGGAATATACTTTTCTTAATCAT
    GTAGGTTTTGCGACTCAAGAAGATATTAGCCCACATATTCCTTTCGCTTTGTTTATGTTA
    TTTCAAATGATGTTTTGTACGATTGCAATTTCTATTTTATCTGGTTCAATCGCTGAGAAA
    ATGAAGTTTATTCCTTATTTATTATTCGTAGTAATATGGACTGCTCTTGTATACAGTCCA
    GTAGCACATTGGGTTTGGGGCGGCGGTTGGATTAACAAACTCGGTGTATTAGATTTCGCT
    GGAGGTACGGTTGTTCATATTACATCAGGTGTTTCTGGTTTAGTATTAGCTATTATGATT
    GGAAAAGGAAACAAACATTCTGAATCAACACCACATAATCTTATCATTACGTTGATTGGC
    GGTATATTCGTGTGGATTGGTTGGTATGGATTTAATGTAGGTAGTGCTTTTACATTTGAT
    AATATTGCGATGCTTGCATTTACAAATACTGTCATTTCAGCCAGTGCAGGTGCTATAGGT
    TGGTTAATTTTAGAATATATTTTTAAAAAGACGACAAGTTTACTTGGACTTTTACTCGGT
    GCATTAGCAGGATTAGTTGTCATTACTCCTGCAGCAGGATATGTAACATATCTTAGTGCA
    ACAATAATGGCTTTAATAGGAGGTATCTGTTGTTATATTGTCATTAATTACATCAAGGTA
    AAACTAAAATATCATGATGCATTAGATGCATTTGGTATTCATGGTGTTGGTGGTATTATT
    GGTGCTGTTTTAACAGCAGTTTTCCAAAGTAAAAAAGCCAATCCTGACATTGAGAATGGC
    TTTATTTATACTGGTGACATACATATTATACTTGTACAAATATTATGTGTAACAGCAGTT
    GTAATTTTTAGTATAGTCATGACGTTTATTATTGCGAAAGTAATTAAATTAATTACACCA
    TTATCTGTTACGGAACAAGAAACGAATATAGGATTAGACAAGATTGTTCACGGTGAACAT
    GCTTACTTTGAAGGTGAGCTAAATAGATTCAATAAACATATTCGATATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1251

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1996 [new locus tag: SAUSA300_RS10975 ]
  • symbol: Amt
  • description: ammonium transporter
  • length: 416
  • theoretical pI: 7.37062
  • theoretical MW: 45478.6
  • GRAVY: 0.852885

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds ammonium transporter (TIGR00836; HMM-score: 405.2)
    and 1 more
    probable ammonium transporter, marine subtype (TIGR03644; HMM-score: 196.7)
  • TheSEED  :
    • Ammonium transporter NrgA
    Nitrogen Metabolism Nitrogen Metabolism - no subcategory Ammonia assimilation  Ammonium transporter
  • PFAM:
    no clan defined Ammonium_transp; Ammonium Transporter Family (PF00909; HMM-score: 375.8)
    and 1 more
    Glycine-zipper (CL0500) Gly-zipper_YMGG; YMGG-like Gly-zipper (PF13441; HMM-score: 7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 11
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.020192
    • TAT(Tat/SPI): 0.000994
    • LIPO(Sec/SPII): 0.028482
  • predicted transmembrane helices (TMHMM): 11

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSMAAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFAGGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVTYLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKANPDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEHAYFEGELNRFNKHIRY

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CodY (repression) regulon, GlnR (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 
    GlnR(TF)important in Nitrogen assimilation; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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