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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2050 [new locus tag: SAUSA300_RS11285 ]
  • pan locus tag?: SAUPAN005376000
  • symbol: SAUSA300_2050
  • pan gene symbol?: tenA
  • synonym:
  • product: TENA/THI-4 family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2050 [new locus tag: SAUSA300_RS11285 ]
  • symbol: SAUSA300_2050
  • product: TENA/THI-4 family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2214625..2215314
  • length: 690
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGAATTTTCACAAAAATTGTACCAAGCTGCAAAGCCAATCATTAATGATATTTATGAG
    GATGACTTTATTCAGAAGATGTTATCCGGAGATATAGGAGCTGACGCATTACGCCATTAC
    TTAAAAGCTGATGCTGCGTATTTAAAAGAATTTACAAACTTATATGCTTTGTTAATTCCA
    AAAATGAATAGCATGAATGACGTAAAATTTTTAGTAGAGCAAATAGAATTTATGGTTGAA
    GGTGAAGTACTTGCGCATGATATTTTAGCGCAAATTGTTGGTGAATCATACGAAGAAATC
    ATTAAAACAAAAGTTTGGCCTCCAAGTGGCGACCATTACATAAAACATATGTATTTCCAA
    GCGCATAGTCGTGAAAATGCTATTTATACAATTGCTGCAATGGCACCTTGTCCATATATT
    TATGCAGAATTAGCTAAGCGCTCTCAAAGTGATCATAAGCTTAATAGAGAAAAAGATACG
    GCAAAATGGTTTGATTTTTATAGTACTGAAATGGATGACATAATTAACGTATTTGAGGCA
    TTAATGAATAAATTAGCCGAATCAATGTCTGACAAGGAATTGGAACAAGTAAAGCAAGTA
    TTTTTAGAAAGTTGCATACATGAGCGTCGATTTTTCAATATGGCTATGACACTAGAACAA
    TGGGAATTTGGAGGAAAAGTAAATGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    690

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2050 [new locus tag: SAUSA300_RS11285 ]
  • symbol: SAUSA300_2050
  • description: TENA/THI-4 family protein
  • length: 229
  • theoretical pI: 4.66731
  • theoretical MW: 26728.4
  • GRAVY: -0.321834

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.99.2?  ExPASy
    Aminopyrimidine aminohydrolase 4-amino-5-aminomethyl-2-methylpyrimidine + H2O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + ammonia Thiamine + H2O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
  • TIGRFAM:
    thiaminase II (TIGR04306; EC 3.5.99.2; HMM-score: 311.3)
  • TheSEED  :
    • Thiaminase II (EC 3.5.99.2) involved in salvage of thiamin pyrimidine moiety
    Cofactors, Vitamins, Prosthetic Groups, Pigments Cofactors, Vitamins, Prosthetic Groups, Pigments - no subcategory Thiamin biosynthesis  Thiaminase II (EC 3.5.99.2) involved in salvage of thiamin pyrimidine moiety
  • PFAM:
    HO (CL0230) TENA_THI-4; TENA/THI-4/PQQC family (PF03070; HMM-score: 138.6)
    and 2 more
    no clan defined Imm30; Immunity protein 30 (PF15565; HMM-score: 18.1)
    EF_hand (CL0220) SPARC_Ca_bdg; Secreted protein acidic and rich in cysteine Ca binding region (PF10591; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001438
    • TAT(Tat/SPI): 0.000339
    • LIPO(Sec/SPII): 0.000183
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEFSQKLYQAAKPIINDIYEDDFIQKMLSGDIGADALRHYLKADAAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIKTKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDTAKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTLEQWEFGGKVND

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: THI-box (transcription termination) regulon
    THI-box(RNA)important in Thiamine biosynthesis; regulatory site identified based on RegPrecise data for N315 RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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