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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2078 [new locus tag: SAUSA300_RS11440 ]
  • pan locus tag?: SAUPAN005416000
  • symbol: murA
  • pan gene symbol?: murA2
  • synonym:
  • product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2078 [new locus tag: SAUSA300_RS11440 ]
  • symbol: murA
  • product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2239704..2240963
  • length: 1260
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGCTCAAGAGGTAATAAAAATAAGAGGTGGACGCACACTAAATGGGGAAGTTAATATC
    AGTGGCGCGAAAAACAGTGCTGTAGCTATCATTCCTGCAACATTATTAGCTCAAGGACAT
    GTGAAATTAGAAGGGTTACCGCAAATCTCTGATGTTAAAACTTTAGTGAGTTTATTAGAA
    GATTTAAATATTAAGGCATCACTAAATGGTACGGAATTAGAAGTTGATACAACTGAAATA
    CAAAATGCTGCATTACCAAATAATAAAGTTGAATCATTACGTGCTTCATACTATATGATG
    GGTGCCATGTTAGGTAGATTTAAAAAATGTGTGATTGGTTTACCGGGAGGATGTCCACTT
    GGGCCACGTCCAATTGATCAACATATTAAAGGATTTAAAGCTTTAGGTGCTGAAATTGAT
    GAATCAAGTACGACATCAATGAAAATCGAAGCTAAAGAGTTAAAAGGTGCACATATTTTT
    CTAGATATGGTTAGCGTAGGTGCAACAATTAACATTATGCTAGCTGCAGTTTATGCAACA
    GGACAAACTGTAATTGAAAATGCTGCTAAAGAACCGGAAGTAGTTGATGTTGCTAACTTC
    TTAACAAGTATGGGAGCTAATATTAAGGGAGCGGGTACATCAACAATTAAAATCAATGGC
    GTCAAAGAATTACATGGTTCTGAGTATCAAGTTATACCTGATAGAATTGAAGCAGGCACA
    TATATGTGTATCGCTGCAGCATGTGGAGAAAATGTTATATTAAATAATATTGTTCCGAAA
    CATGTAGAAACATTAACTGCTAAATTTAGTGAATTAGGTGTGAATGTTGATGTAAGAGAC
    GAAAGAATTCGCATCAATAATAATGCGCCATATCAATTTGTTGATATTAAAACACTAGTA
    TATCCAGGTTTTGCTACTGATTTGCAACAGCCTATTACACCATTATTATTTATGGCAAAT
    GGTCCTTCATTTGTAACTGATACGATTTATCCGGAGCGTTTTAAACATGTTGAAGAATTA
    AAGCGCATGGGTGCAAATATTGAAGTTGACGAAGGCACAGCAACGATAAAACCATCAACA
    TTACATGGTGCTGAAGTTTATGCTAGCGATTTAAGAGCAGGAGCTTGTTTAATTATTGCT
    GGTTTAATTGCTGAGGGTGTAACTACAATTTACAATGTTAAACATATTTATAGAGGTTAT
    ACAGATATTGTAGAACACTTAAAAGCTTTAGGTGCAGATATTTGGACGGAAACTGTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2078 [new locus tag: SAUSA300_RS11440 ]
  • symbol: MurA
  • description: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
  • length: 419
  • theoretical pI: 5.46141
  • theoretical MW: 45074.7
  • GRAVY: 0.072315

⊟Function[edit | edit source]

  • reaction:
    EC 2.5.1.7?  ExPASy
    UDP-N-acetylglucosamine 1-carboxyvinyltransferase Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylglucosamine 1-carboxyvinyltransferase (TIGR01072; EC 2.5.1.7; HMM-score: 526.9)
    and 1 more
    Metabolism Amino acid biosynthesis Aromatic amino acid family 3-phosphoshikimate 1-carboxyvinyltransferase (TIGR01356; EC 2.5.1.19; HMM-score: 87.8)
  • TheSEED  :
    • UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan Biosynthesis  UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7)
    and 1 more
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis  UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7)
  • PFAM:
    EPT_RTPC (CL0290) EPSP_synthase; EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase) (PF00275; HMM-score: 300.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.018228
    • TAT(Tat/SPI): 0.001295
    • LIPO(Sec/SPII): 0.001594
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLEGLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGAMLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIFLDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKINGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNVDVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERFKHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYNVKHIYRGYTDIVEHLKALGADIWTETV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAUSA300_2142(asp23)alkaline shock protein 23  [1] (data from MRSA252)
    SAUSA300_1080(ftsZ)cell division protein FtsZ  [1] (data from MRSA252)
    SAUSA300_2204(rplC)50S ribosomal protein L3  [1] (data from MRSA252)
    SAUSA300_1134(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    SAUSA300_2199(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    SAUSA300_1149(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    SAUSA300_2198(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Thomas Böttcher, Stephan A Sieber
    Showdomycin as a versatile chemical tool for the detection of pathogenesis-associated enzymes in bacteria.
    J Am Chem Soc: 2010, 132(20);6964-72
    [PubMed:20433172] [WorldCat.org] [DOI] (I p)    Soma Ghosh, Jyoti Prava, Himanshu Bhusan Samal, Mrutyunjay Suar, Rajani Kanta Mahapatra
    Comparative genomics study for the identification of drug and vaccine targets in Staphylococcus aureus: MurA ligase enzyme as a proposed candidate.
    J Microbiol Methods: 2014, 101;1-8
    [PubMed:24685600] [WorldCat.org] [DOI] (I p)
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