PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2276 [new locus tag: SAUSA300_RS12580 ]
• pan locus tag^?: SAUPAN005815000
• symbol: SAUSA300_2276
• pan gene symbol^?: —
• synonym:
• product: M20/M25/M40 family peptidase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2276 [new locus tag: SAUSA300_RS12580 ]
• symbol: SAUSA300_2276
• product: M20/M25/M40 family peptidase
• replicon: chromosome
• strand: -
• coordinates: 2446970..2448091
• length: 1122
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912793 NCBI
• RefSeq: YP_494911 NCBI
• BioCyc: see SAUSA300_RS12580
• MicrobesOnline: 1293791 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGGGATTAAAAGATCAAGCTGTTACCTGGAGAAGGTATTTTCATCAATTTCCAGAACTA
  TCCGATAAAGAATTTAAGACTACACAAAAAATTAAAGATATTTTAACAGAACATCATATT
  AGAATATTAGACTTACCACTTGCGACTGGGCTTGTTGCTGAAGTGGGACAAGGTCTGAGT
  TGTATAGCTGTTCGGGCTGACATTGATGCTTTACCGATTCAAGAGCTTGTTGAACAGGAT
  TTTAAATCTGAAAATGAAGGTGTTATGCATGCGTGTGGTCATGATATTCACATGGCTAGT
  ATTTTGGCTACAGCTGTAAAATTAAAAGAAATTGAGGGCACTCTTACTGGGCGTGTTAAA
  TTCATTTTTCAATCAGCAGAAGAACTAGGACATGGCGCTTTTAAAATTATTGAAACACAT
  GCATTAAAAGATGTACAAGCAGTATTGGGTTTTCATAATGACCCATCGCGTTCGGTAGGT
  ACATTTGCAATCAAAACAGGGGCAATTACATCAGCAGTAGATCGTTTTGAGTTTCATATT
  AAAGGCGTGGGTGGTCATGCTGCAAAGCCAGAACAATGCAACGATCCAGTTATTGTGTTG
  GCGCAATTGATTAATAGTATTCAATCCATAGTTAGTAGAAATCTATCTGCGTTTGATGAA
  GCGGTAGTAACAATTGGACAAATATCATGTGGTAATACTTGGAATGTCATTGCTGATCAT
  GCGTATGTACAAGGCACAGTGCGTAGTTTTGATCCTGTGGTACGAAAGCTTGTAGAAACA
  CGATTACAAGATATTGCGGACGGATTGGCTCAAGCATATAATATGAAAATCAATTTAAAT
  TATACGCATTTACCTGGCGCTGTGATGAATGATGAGGCATTGACGCATAAAGCAATTGCA
  GTAGCCCAACATGTTGGATATAAGGTGGAAATGATGGAACAGCCGCTTACGATTGGAGAA
  GATTTTTCAGGTTATAGTCAACATTTTCCAAGTGTATTTGCGTTAATTGGTTCTCATAGT
  GAATATGATTTACATCATCCTCAATATAAACCGGATGAAAGGATTCTGGAAAAAGTACCT
  GAGTATTTTGTTGAATTTGTAAAGAGACTATTGCATGAATAA
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  1122
  

⊟Protein

⊟General

• locus tag: SAUSA300_2276 [new locus tag: SAUSA300_RS12580 ]
• symbol: SAUSA300_2276
• description: M20/M25/M40 family peptidase
• length: 373
• theoretical pI: 6.16491
• theoretical MW: 41468
• GRAVY: -0.0922252

⊟Function

• reaction:
  EC 3.5.1.14?  ExPASy
  N-acyl-aliphatic-L-amino acid amidohydrolase An N-acyl-aliphatic-L-amino acid + H₂O = an aliphatic L-amino acid + a carboxylate An N-acetyl-L-cysteine-S-conjugate + H₂O = an L-cysteine-S-conjugate + acetate
• TIGRFAM:
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides amidohydrolase (TIGR01891; HMM-score: 413.3)
  and 6 more
  Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01246; EC 3.5.1.18; HMM-score: 45.3)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 36.9)
  amidase, hydantoinase/carbamoylase family (TIGR01879; EC 3.5.-.-; HMM-score: 28.2)
  Metabolism Amino acid biosynthesis Glutamate family acetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 28)
  Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01900; EC 3.5.1.18; HMM-score: 23)
  putative selenium metabolism hydrolase (TIGR03526; HMM-score: 13.2)
• TheSEED  :
  • N-acetyl-L,L-diaminopimelate deacetylase homolog (EC 3.5.1.18)
• PFAM:
  Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 99.1)
  and 2 more
  no clan defined M20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 36.5)
  JAB (CL0366) RadC; RadC-like JAB domain (PF04002; HMM-score: 12.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005607
  • TAT(Tat/SPI): 0.00056
  • LIPO(Sec/SPII): 0.000224
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161218 NCBI
• RefSeq: YP_494911 NCBI
• UniProt: A0A0H2XG69 UniProt

⊟Protein sequence

• MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILDLPLATGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASILATAVKLKEIEGTLTGRVKFIFQSAEELGHGAFKIIETHALKDVQAVLGFHNDPSRSVGTFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAFDEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMKINLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFALIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications