From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2332 [new locus tag: SAUSA300_RS12885 ]
  • pan locus tag?: SAUPAN005914000
  • symbol: SAUSA300_2332
  • pan gene symbol?: —
  • synonym:
  • product: heat shock protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2332 [new locus tag: SAUSA300_RS12885 ]
  • symbol: SAUSA300_2332
  • product: heat shock protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2507834..2508262
  • length: 429
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGATATTGAACTTCAATCAATTCGAGAATCAAAACTTTTTTAACGCTAATCCAAGTGAT
    ACATTTAAAGATTTAGGTAAACAAGTATTTAATTACTTTTCAACACCTTCATTTGTAACG
    AATATATATGAAACAGACGAATTATATTACTTAGAAGCTGAACTAGCAGGTGTAAATAAA
    GAAGATATTAGTATCGATTTCAATAATAATACGCTCACTATTCAAGCTACTAGAAGCGCA
    AAATACAAATCTGAACAACTCATTTTAGATGAGCGTAACTTCGAATCATTAATGCGTCAA
    TTTGATTTTGAAGCTGTTGATAAGCAACATATTACTGCTAGTTTTGAAAATGGGTTATTA
    ACCATTACCTTGCCTAAAATCAAACCAAGCAATGAAACTACTTCATCAACATCTATTCCA
    ATTTCATAG
    60
    120
    180
    240
    300
    360
    420
    429

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2332 [new locus tag: SAUSA300_RS12885 ]
  • symbol: SAUSA300_2332
  • description: heat shock protein
  • length: 142
  • theoretical pI: 4.21588
  • theoretical MW: 16319
  • GRAVY: -0.460563

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Molecular chaperone, small heat shock protein
  • PFAM:
    HSP20 (CL0190) HSP20; Hsp20/alpha crystallin family (PF00011; HMM-score: 64.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.53
    • Cellwall Score: 8.75
    • Extracellular Score: 0.7
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00553
    • TAT(Tat/SPI): 0.000202
    • LIPO(Sec/SPII): 0.000649
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MILNFNQFENQNFFNANPSDTFKDLGKQVFNYFSTPSFVTNIYETDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFDFEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_2332&oldid=99662"