From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2349 [new locus tag: SAUSA300_RS12965 ]
  • pan locus tag?: SAUPAN005935000
  • symbol: SAUSA300_2349
  • pan gene symbol?: —
  • synonym:
  • product: formate/nitrite transporter family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2349 [new locus tag: SAUSA300_RS12965 ]
  • symbol: SAUSA300_2349
  • product: formate/nitrite transporter family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2526277..2527101
  • length: 825
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    GTGATAGAAAATAAGAAAACAGTTGAAGATACATATTCAACAGGCGCAATTGTTGATTCA
    ATATCATCTTCAGTACAAATGAAGCAAGTAATGGTACAGCAAACACCTGGCCGATATATG
    CTTAAAGCAATGATGGCCGGATTTTTACTCTCAATCGTTACTGTATTTATGTTCGGAATA
    AAGACGCAGTTTGCGAGTACTCACGTAGATGGACTTATCAATTTAATGGGAGCCATTGCG
    TTTAGTTTAGGTTTGATTTTAGTTGTATTAACTAACTCTGAATTATTAACAAGTAACTTC
    ATGTACTTTACAGTAGGCTGGTATTATAAAGTCGTTTCTGTTAAGAAGATGACTTGGATT
    TTATTATATTGTTTCTTAGGAAATATTTTAGGTGGGTTTGTGTTATTTTTCTTAATGAAA
    TTTGCGCATGTCATGACACCTGAAATGACACAAGCTTTAACAGCTTTAGTTCAGAAAAAG
    ACTGTTGATTCAACATGGTTGAATATTTTTACAAAAGGTATTTTCTGTAACTTCTTTATT
    AATATTGGTATTTTCATCTCTATGCAATTCAAAGGCGGCTTAACAAAGGCATTCTTCATA
    GCTTGTGGTGTCGTGGTCTTTGTATACATGGGATATGAGCACGTTGTGTTCAATGCAGGT
    CTATATGCAGGTATGGTATTCTTTAACCTTGATGCAGTTTCATGGTTACACGTTTTAAAA
    AATATCGTTTTTGCTTTCTTAGGTAACTTTGTCGGTGGCGGTATTTTTGTAGGTCTCGTT
    TATGCTTTCTTAAATGGTAAGCGTAATCGTTTAGAGCAACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    825

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2349 [new locus tag: SAUSA300_RS12965 ]
  • symbol: SAUSA300_2349
  • description: formate/nitrite transporter family protein
  • length: 274
  • theoretical pI: 9.72847
  • theoretical MW: 30669.4
  • GRAVY: 0.762774

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids formate transporter FocA (TIGR04060; HMM-score: 137)
    Metabolism Transport and binding proteins Anions formate/nitrite transporter (TIGR00790; HMM-score: 136.2)
  • TheSEED  :
    • formate/nitrite transporter family protein
  • PFAM:
    no clan defined Form_Nir_trans; Formate/nitrite transporter (PF01226; HMM-score: 166.8)
    and 1 more
    DUF872; Eukaryotic protein of unknown function (DUF872) (PF05915; HMM-score: 6.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003313
    • TAT(Tat/SPI): 0.000288
    • LIPO(Sec/SPII): 0.003158
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAMMAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMYFTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKKTVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFNAGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_2349&oldid=101842"