PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2392 [new locus tag: SAUSA300_RS13240 ]
• pan locus tag^?: SAUPAN005999000
• symbol: opuCb
• pan gene symbol^?: opuCB
• synonym:
• product: glycine betaine/carnitine/choline ABC transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2392 [new locus tag: SAUSA300_RS13240 ]
• symbol: opuCb
• product: glycine betaine/carnitine/choline ABC transporter
• replicon: chromosome
• strand: -
• coordinates: 2575271..2575906
• length: 636
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913918 NCBI
• RefSeq: YP_495027 NCBI
• BioCyc: see SAUSA300_RS13240
• MicrobesOnline: 1293907 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGATTGAGTTCCTACATGAACATGGTGGACAGTTGATGTCGAAAACACTGGAACATTTC
  TATATTTCTATAGTGGCATTATTACTTGCCATCATTGTTGCAGTACCTATAGGCATTTTA
  TTATCAAAAACAAAGCGAACTGCCAATATTGTATTAACTGTGGCAGGTGTCTTACAAACT
  ATTCCAACACTAGCTGTACTTGCTATTATGATACCGATTTTTGGTGTTGGTAAAACGCCT
  GCAATTGTAGCGCTATTTATTTATGTATTATTACCTATTTTAAATAACACGGTACTCGGT
  GTTCAAAATATTGATAGCAACATTAAAGAAGCTGGAAAAAGTATGGGAATGACACAATTT
  CAATTGATGAAGGATGTTGAATTGCCGTTAGCATTGCCGCTTATCATTGGTGGCATTCGT
  TTGTCATCTGTGTATGTAATTAGTTGGGCTACACTTGCAAGTTATGTAGGTGCGGGTGGA
  TTAGGTGATTTCATTTTCAATGGTTTAAATTTATATGATCCACTGATGATTGTAACTGCA
  ACGGTACTCGTTACTGCACTAGCATTAGGTGTTGATGCCTTATTAGCTTTAGTTGAAAAA
  TGGGTAGTTCCCAAAGGCTTAAAAGTATCTGGATAA
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  636
  

⊟Protein

⊟General

• locus tag: SAUSA300_2392 [new locus tag: SAUSA300_RS13240 ]
• symbol: OpuCb
• description: glycine betaine/carnitine/choline ABC transporter
• length: 211
• theoretical pI: 8.84699
• theoretical MW: 22385.9
• GRAVY: 1.03744

⊟Function

• reaction:
  EC 3.6.3.32?  ExPASy
  Quaternary-amine-transporting ATPase ATP + H₂O + quaternary amine(Out) = ADP + phosphate + quaternary amine(In)
• TIGRFAM:
  choline ABC transporter, permease protein (TIGR03416; HMM-score: 95.1)
  and 8 more
  Metabolism Transport and binding proteins Anions nitrate ABC transporter, permease protein (TIGR01183; HMM-score: 60.2)
  Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 49.2)
  NifC-like ABC-type porter (TIGR01581; HMM-score: 25.9)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 23.4)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysT (TIGR02139; HMM-score: 21.8)
  Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 19.9)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 16.4)
  ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 10.1)
• TheSEED  :
  • Choline ABC transport system, permease protein OpuBB
  Stress Response Osmotic stress Choline and Betaine Uptake and Betaine Biosynthesis  Osmotically activated L-carnitine/choline ABC transporter, permease protein OpuCB
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 79.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 5
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004266
  • TAT(Tat/SPI): 0.000244
  • LIPO(Sec/SPII): 0.00151
• predicted transmembrane helices (TMHMM): 5

⊟Accession numbers

• GI: 87162395 NCBI
• RefSeq: YP_495027 NCBI
• UniProt: A0A0H2XKJ8 UniProt

⊟Protein sequence

• MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKTKRTANIVLTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQNIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGGLGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: opuCd < opuCc < opuCb < opuCa

⊟Regulation

• regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications