PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2396 [new locus tag: SAUSA300_RS13270 ]
• pan locus tag^?: SAUPAN006007000
• symbol: pnbA
• pan gene symbol^?: pnbA
• synonym:
• product: para-nitrobenzyl esterase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2396 [new locus tag: SAUSA300_RS13270 ]
• symbol: pnbA
• product: para-nitrobenzyl esterase
• replicon: chromosome
• strand: +
• coordinates: 2580823..2582175
• length: 1353
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914130 NCBI
• RefSeq: YP_495031 NCBI
• BioCyc: see SAUSA300_RS13270
• MicrobesOnline: 1293911 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  1321
  
  ATGAAGATTAATACTACAGGTGGTCAAATTCATGGTATTACACAAGATGGTTTAGATATC
  TTCTTAGGCATTCCTTATGCAGAACCACCAGTTCATGACAATCGCTTTAAACATTCTACG
  TTAAAAACACAATGGTCAGAGCCAATTGATGCAACTGAAATACAACCCATCCCACCGCAA
  CCAGACAACAAATTAGAAGATTTTTTCTCCTCACAATCTACAACTTTTACTGAACATGAA
  GACTGTTTATATCTAAATATTTGGAAACAACATAATGATCAGACGAAGAAACCTGTCATC
  ATTTATTTTTATGGTGGTAGTTTTGAAAATGGTCATGGTACAGCCGAACTCTATCAACCG
  GCACATTTAGTACAAAATAACGACATTATCGTTATTACATGCAATTATCGTTTAGGCGCA
  TTAGGATATTTAGACTGGTCATATTTTAATAAAGATTTTCATTCCAATAATGGCCTTTCA
  GATCAAATCAATGTCATAAAATGGGTGCATCAATTTATTGAATCCTTCGGTGGCGACGCT
  AATAACATTACTTTAATGGGTCAGTCTGCAGGCAGTATGAGCATTTTGACTTTACTTAAA
  ATACCTGACATTGAGCCATACTTCCATAAAGTCGTTCTACTAAGTGGCGCACTACGATTA
  GACACCCTTGAGAGTGCACGCAATAAAGCACAACATTTCCAAAAAATGATGCTCGATTAT
  TTAGATACAGATGATGTTACATCATTATCGACAAATGATATTCTTATGCTGATGGCGAAG
  CTAAAACAATCTCGAGGACCTTCTAAAGGGCTTGATTTAATATATGCGCCTATTAAAACA
  GATTATATACAAAATAATTATCCAACAACGAAACCAATTTTTGCATGTTATACAAAAGAT
  GAAGGCGATATTTATATTACTAGTGAACAGAAAAAATTATCGCCGCAACGCTTTATCGAC
  ATTATGGAATTAAATGATATTCCTTTAAAATACGAAGATGTTCAGACGGCGAAGCAACAA
  TCTTTAGCGATTACACATTGTTATTTCAAACAGCCGATGAAGCAATTTTTACAACAACTC
  AATATACAAGATTCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCT
  AGCGCACACTATCGCAGTGCTTACCATATTCTAGATATGGTATTTTGGTTCGGAAACTTA
  CAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAATTTTTAAGTCGACAA
  ATGCAAAATGATTTAGCTAATTTTGCTAAAAGCGGTAAAATGCCTTGGCCGATGTATCAT
  AATGAACGTCGCTATTATCGCACATATCAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2396 [new locus tag: SAUSA300_RS13270 ]
• symbol: PnbA
• description: para-nitrobenzyl esterase
• length: 450
• theoretical pI: 6.50843
• theoretical MW: 51968.5
• GRAVY: -0.461111

⊟Function

• reaction:
  EC 3.1.1.-?  ExPASy
• TIGRFAM:
• TheSEED  :
  • Carboxylesterase type B (EC 3.1.1.-)
• PFAM:
  AB_hydrolase (CL0028) COesterase; Carboxylesterase family (PF00135; HMM-score: 253.6)
  and 4 more
  Abhydrolase_3; alpha/beta hydrolase fold (PF07859; HMM-score: 27.3)
  Peptidase_S9; Prolyl oligopeptidase family (PF00326; HMM-score: 20.5)
  Say1_Mug180; Steryl acetyl hydrolase (PF10340; HMM-score: 17.6)
  SGNH_hydrolase (CL0264) ALGX; SGNH hydrolase-like domain, acetyltransferase AlgX (PF16822; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.01266
  • TAT(Tat/SPI): 0.001294
  • LIPO(Sec/SPII): 0.002138
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160859 NCBI
• RefSeq: YP_495031 NCBI
• UniProt: A0A0H2XHF0 UniProt

⊟Protein sequence

• MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQWSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIYFYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLSDQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGALRLDTLESARNKAQHFQKMMLDYLDTDDVTSLSTNDILMLMAKLKQSRGPSKGLDLIYAPIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDVQTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILDMVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications