PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2435
• pan locus tag^?: SAUPAN006103000
• symbol: SAUSA300_2435
• pan gene symbol^?: sasG
• synonym:
• product: cell wall surface anchor family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2435
• symbol: SAUSA300_2435
• product: cell wall surface anchor family protein
• replicon: chromosome
• strand: -
• coordinates: 2622904..2623494
• length: 591
• essential: unknown

⊟Accession numbers

• Gene ID: 3914510 NCBI
• RefSeq: YP_495069 NCBI
• BioCyc:
• MicrobesOnline: 1293950 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  GTGATCGAAGAGCCAGTGGATGATGTGATTAAACACGGACCAAAAACGGGTACACCAGAA
  ACAAAAACAGTAGAGATACCGTTTGAAACAAAACGTGAGTTTAATCCAAAATTACAACCT
  GGTGAAGAGCGAGTGAAACAAGAAGGACAACCAGGAAGTAAGACAATCACAACACCAATC
  ACAGTGAACCCATTAACAGGTGAAAAAGTTGGCGAGGGTCAACCAACAGAAGAGATCACA
  AAACAACCAGTAGATAAGATTGTAGAGTTCGGTGGAGAGAAACCAAAAGATCCAAAAGGA
  CCTGAAAACCCAGAGAAGCCGAGCAGACCAACTCATCCAAGTGGCCCAGTAAATCCTAAC
  AATCCAGGATTATCGAAAGACAGAGCAAAACCAAATGGCCCAGTTCATTCAATGGATAAA
  AATGATAAAGTTAAAAAATCTAAAATTGCTAAAGAATCAGTAGCTAATCAAGAGAAAAAA
  CGAGCAGAATTACCAAAAACAGGTTTAGAAAGCACGCAAAAAGGTTTGATCTTTAGTAGT
  ATAATTGGAATTGCTGGATTAATGTTATTGGCTCGTAGAAGAAAGAATTAA
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  591
  

⊟Protein

⊟General

• locus tag: SAUSA300_2435
• symbol: SAUSA300_2435
• description: cell wall surface anchor family protein
• length: 196
• theoretical pI: 10.2538
• theoretical MW: 21414.3
• GRAVY: -1.05204

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 25.1)
  pullulanase, extracellular (TIGR02102; HMM-score: 23.2)
• TheSEED  :
  • Virulence-associated cell-wall-anchored protein SasG (LPXTG motif), binding to squamous nasal epithelial cells
  Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Virulence-associated cell-wall-anchored protein SasG (LPXTG motif), binding to squamous nasal epithelial cells
• PFAM:
  G5 (CL0593) G5; G5 domain (PF07501; HMM-score: 55.6)
  and 3 more
  no clan defined Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 28.6)
  2C_adapt; 2-cysteine adaptor domain (PF08793; HMM-score: 16.3)
  G5 (CL0593) SasG_E; E domain (PF17041; HMM-score: 12.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 10
  • Extracellular Score: 0
  • Internal Helix: 1
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.83
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.012341
  • TAT(Tat/SPI): 0.005271
  • LIPO(Sec/SPII): 0.004295
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161164 NCBI
• RefSeq: YP_495069 NCBI
• UniProt: A0A0H2XG25 UniProt

⊟Protein sequence

• MIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSGPVNPNNPGLSKDRAKPNGPVHSMDKNDKVKKSKIAKESVANQEKKRAELPKTGLESTQKGLIFSSIIGIAGLMLLARRRKN

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications