PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2442 [new locus tag: SAUSA300_RS13545 ]
• pan locus tag^?: SAUPAN006130000
• symbol: gntP
• pan gene symbol^?: gntP
• synonym:
• product: gluconate permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2442 [new locus tag: SAUSA300_RS13545 ]
• symbol: gntP
• product: gluconate permease
• replicon: chromosome
• strand: -
• coordinates: 2636270..2637628
• length: 1359
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913509 NCBI
• RefSeq: YP_495076 NCBI
• BioCyc: see SAUSA300_RS13545
• MicrobesOnline: 1293957 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGTTTAACGAAATATGGCCGTTAATCAGTGTTGTTTTAGGTATCGTTATTTTATTAGTA
  TTAATTATTGGGTTTAAATTAAATACATTTATTTCATTAATTATCACATCGATGATTACA
  GCGTTAATGCTAGGTATACCATTGACTAAAATTATGGAGACGATTGAGAAAGGAATGGGC
  AGTACGCTCGGCCACATTGCACTGATATTCGGTTTAGGTGCCATACTTGGGAAATTGCTT
  GCTGATGGTGGTGGTGCAACTCGAATCGCAGACACGTTGATTCAAAAATTTGGTCAAAAA
  CATGTACAATGGGCAATGCTTGTTGCAGCATTTATCGTCGGTATTGCATTGTTCTTTGAA
  GTAGGTTTAGTCTTACTGATTCCATTAGTATTTACAGTAGCAAAACGCGCAAATGTTTCA
  GTGTTAAAACTAGGACTACCTATGGTAACAGCTTTATCAGTGACACATGGCTTTTTACCA
  CCACATCCAGGACCGGTAGTCATCGCAAAAGAATTAAAGGCAAATGTTGGAGATGTATTA
  CTGTATGGTATGATTATTGCCATTCCAGTTACACTCATTGCAGGGCCTATATTTAACAAA
  GTTGCACAAAAAATGATTCCGTCTGCGTATACACGAGAAGGCGATATTTCAGCGTTAGGT
  GCACAAAAAGAATTTACGGATCAAGAGATGCCAGGATTTGGTATGAGTTTATTAACAGCA
  ACATTACCAGTCATATTAATGTTAGTGTCTACGATAACGCAACTTGTAACTGGGCATGAC
  AAACCTACAAATCTATTTGAATCTATCATTTATATGATAGGAACAGCAGGGACAGCCATG
  CTAATAGCAGTATTATTTGCAATCGTTACGATGGGATTAATGAGAAAGCGTAAGATGAAT
  CATATTATGGAATCAGTGACGAATGCGATTTATCCAATCGGAATGATGTTATTGATTATT
  GGCGGTGGCGGTACATTTAAACAAGTATTAATTGACGGCGGTGTCGGAAATACAATTGCT
  AAAATGTTTGAAGGTACAGAGATGTCTCCGATTTTACTAGCATGGATTGTTGCAGCTGTG
  CTACGTATCGCATTAGGTTCGGCTACAGTAGCTGCGATTTCAACTACAGGTATTGTCTTA
  CCATTATTACAATCATCAGATGTAAATGTTGCATTAGTTGTACTTGCGATAGGTGCAGGT
  AGTGTGATTTTGTCTCATGTAAATGACGCAGGATTTTGGATGTTTAAAGAATATTTTGGT
  TTAACTGTAAAAGAAACATTCTTAACATGGTCATTATTAGAAACGATTATTTCCGTTTCA
  GGTATCATCTTCATTTTATTCATTAGCTTATTTGTTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2442 [new locus tag: SAUSA300_RS13545 ]
• symbol: GntP
• description: gluconate permease
• length: 452
• theoretical pI: 9.2587
• theoretical MW: 48102.9
• GRAVY: 1.02942

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids transporter, gluconate:H+ symporter (GntP) family (TIGR00791; HMM-score: 600.4)
• TheSEED  :
  • Gluconate permease, Bsu4004 homolog
  Carbohydrates Monosaccharides D-gluconate and ketogluconates metabolism  Gluconate permease, Bsu4004 homolog
• PFAM:
  IT (CL0182) GntP_permease; GntP family permease (PF02447; HMM-score: 570.6)
  and 4 more
  CitMHS; Citrate transporter (PF03600; HMM-score: 72.3)
  Na_H_antiport_2; Na+-H+ antiporter family (PF13726; HMM-score: 16.9)
  no clan defined Tim17; Tim17/Tim22/Tim23/Pmp24 family (PF02466; HMM-score: 10.6)
  IT (CL0182) DctM; Tripartite ATP-independent periplasmic transporter, DctM component (PF06808; HMM-score: 7.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001856
  • TAT(Tat/SPI): 0.000172
  • LIPO(Sec/SPII): 0.001043
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 87160554 NCBI
• RefSeq: YP_495076 NCBI
• UniProt: A0A0H2XES1 UniProt

⊟Protein sequence

• MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALMLGIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHVQWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLPPHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISALGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTAGTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGGVGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVALVVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISLFV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: gntP < gntK < gntR < SAUSA300_2445

⊟Regulation

• regulators: CcpA regulon, GntR (repression) regulon

  CcpA	(TF)	important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise
  GntR	(TF)	important in Gluconate utilization; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications