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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2483 [new locus tag: SAUSA300_RS13790 ]
  • pan locus tag?: SAUPAN006206000
  • symbol: SAUSA300_2483
  • pan gene symbol?: mvaA
  • synonym:
  • product: hydroxymethylglutaryl-CoA reductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2483 [new locus tag: SAUSA300_RS13790 ]
  • symbol: SAUSA300_2483
  • product: hydroxymethylglutaryl-CoA reductase
  • replicon: chromosome
  • strand: -
  • coordinates: 2681398..2682675
  • length: 1278
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCAAAATTTAGATAAGAATTTTCGACATTTATCTCGTAAAGAAAAGTTACAACAATTG
    GTTGATAAGCAATGGTTATCAGAAGAACAATTCGACATTTTACTGAATCATCCATTAATC
    GATGAAGAAGTAGCCAATAGTTTAATTGAAAATGTCATCGCGCAAGGTGCATTACCCGTT
    GGATTATTACCGAATATCATTGTGGACGATAAGGCATATGTTGTACCTATGATGGTGGAA
    GAGCCTTCAGTTGTCGCTGCAGCTAGTTATGGTGCAAAGCTAGTGAATCAGACTGGCGGA
    TTTAAAACGGTATCTTCTGAACGTATTATGATAGGTCAAATCGTCTTTGATGGCGTTGAC
    GATACTGAAAAATTATCAGCAGACATTAAAGCTTTAGAAAAGCAAATTCATAAAATTGCG
    GATGAGGCATATCCTTCTATTAAAGCGCGTGGTGGTGGTTACCAACGTATAGCGATTGAT
    ACATTTCCTGAGCAACAGTTACTATCTTTAAAAGTATTTGTTGATACGAAAGATGCTATG
    GGCGCTAATATGCTTAATACGATTTTAGAGGCCATAACTGCATTTTTAAAAAATGAATTT
    CCGCAAAGCGACATTTTAATGAGTATTTTATCCAATCATGCAACAGCGTCCGTTGTTAAA
    GTTCAAGGCGAAATTGATGTTAAAGATTTAGCAAGGGGCGAGAGAACTGGAGAAGAGGTT
    GCCAAACGAATGGAACGTGCTTCTGTATTGGCCCAAGTAGATATTCATCGTGCAGCAACA
    CATAATAAAGGTGTTATGAATGGCATACATGCTGTTGTTTTAGCAACAGGAAATGATACG
    CGTGGTGCAGAAGCAAGTGCGCATGCATACGCGAGTCGTGACGGACAGTATCGTGGTATT
    GCTACATGGCGTTACGATCAAGATCGTCAACGATTGATTGGTACAATTGAAGTGCCTATG
    ACATTGGCAATCGTTGGCGGTGGTACAAAAGTATTACCAATTGCTAAAGCTTCATTAGAG
    CTACTAAATGTAGAGTCAGCACAAGAATTAGGTCATGTAGTTGCTGCCGTTGGTTTAGCG
    CAAAACTTTGCAGCATGTCGCGCGCTTGTGTCAGAAGGTATTCAACAAGGTCATATGAGT
    TTACAATATAAATCATTAGCTATCGTTGTAGGGGCAAAAGGTGATGAAATTGCTAAAGTA
    GCTGAAGCTTTGAAAAAAGAACCCCGTGCAAATACACAAGCAGCGGAACATATTTTACAA
    GAAATTAGACAACAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1278

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2483 [new locus tag: SAUSA300_RS13790 ]
  • symbol: SAUSA300_2483
  • description: hydroxymethylglutaryl-CoA reductase
  • length: 425
  • theoretical pI: 5.98668
  • theoretical MW: 46236.6
  • GRAVY: -0.109647

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.88?  ExPASy
    Hydroxymethylglutaryl-CoA reductase (R)-mevalonate + CoA + 2 NAD+ = 3-hydroxy-3-methylglutaryl-CoA + 2 NADH
  • TIGRFAM:
    Metabolism Energy metabolism Other hydroxymethylglutaryl-CoA reductase, degradative (TIGR00532; EC 1.1.1.88; HMM-score: 335)
    and 2 more
    Metabolism Central intermediary metabolism Other hydroxymethylglutaryl-CoA reductase (NADPH) (TIGR00533; EC 1.1.1.34; HMM-score: 63.6)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids 3-hydroxy-3-methylglutaryl-coenzyme A reductase (TIGR00920; HMM-score: 53.7)
  • TheSEED  :
    • Hydroxymethylglutaryl-CoA reductase (EC 1.1.1.34)
    Fatty Acids, Lipids, and Isoprenoids Isoprenoids Archaeal lipids  Hydroxymethylglutaryl-CoA reductase (EC 1.1.1.34)
    and 1 more
    Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoid Biosynthesis  Hydroxymethylglutaryl-CoA reductase (EC 1.1.1.34)
  • PFAM:
    no clan defined HMG-CoA_red; Hydroxymethylglutaryl-coenzyme A reductase (PF00368; HMM-score: 341.2)
    and 2 more
    DUF2493; Protein of unknown function (DUF2493) (PF10686; HMM-score: 14.5)
    UPF0180; Uncharacterised protein family (UPF0180) (PF03698; HMM-score: 14.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001125
    • TAT(Tat/SPI): 0.000088
    • LIPO(Sec/SPII): 0.000133
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQNLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEEVANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFKTVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAIDTFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASVVKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLATGNDTRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPIAKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGAKGDEIAKVAEALKKEPRANTQAAEHILQEIRQQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Carl J Balibar, Xiaoyu Shen, Jianshi Tao
    The mevalonate pathway of Staphylococcus aureus.
    J Bacteriol: 2009, 191(3);851-61
    [PubMed:19028897] [WorldCat.org] [DOI] (I p)
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