PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2492 [new locus tag: SAUSA300_RS13845 ]
• pan locus tag^?: SAUPAN006218000
• symbol: SAUSA300_2492
• pan gene symbol^?: —
• synonym:
• product: acetyltransferase family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2492 [new locus tag: SAUSA300_RS13845 ]
• symbol: SAUSA300_2492
• product: acetyltransferase family protein
• replicon: chromosome
• strand: -
• coordinates: 2695273..2695872
• length: 600
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914590 NCBI
• RefSeq: YP_495126 NCBI
• BioCyc: see SAUSA300_RS13845
• MicrobesOnline: 1294007 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  
  ATGACTGAAAAAGAAAAAATGTTAGCAGAAAAATGGTACGATGCAAACTTTGATCAATAC
  TTAATCAATGAACGTGCACGAGCGAAAGATATTTGCTTTGAATTAAATCATACAAGACCG
  AGTGCTACAAATAAAAGAAAGGAATTAATTGATCAATTATTTCAAACAACAACAGACAAT
  GTAAGTATTTCGATTCCTTTTGATACAGATTATGGTTGGAACGTTAAACTAGGAAAAAAT
  GTCTATGTAAACACCAATTGTTACTTTATGGATGGTGGACAGATTACAATTGGCGATAAT
  GTTTTTATAGGACCTAATTGTGGATTCTACACAGCGACACATCCACTTAATTTCCATCAT
  AGAAATGAAGGTTTTGAAAAAGCCGGACCCATTCATATTGGCAGTAATACTTGGTTTGGC
  GGTCATGTAGCCGTGTTACCAGGGGTGACGATTGGAGAAGGCAGTGTGATTGGTGCTGGT
  AGTGTTGTCACTAAAGATATTCCGCCACACAGCTTAGCGGTTGGAAACCCTTGTAAAGTC
  GTTCGTAAAATTGATAATGACCTACCATCCGAAACATTAAATGACGAAACGATAAAGTAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  

⊟Protein

• 3FTT
  PDB
• 3V4E
  PDB
• 4DCL
  PDB
• 4EGG
  PDB
• 5U2K
  PDB
• 5V0Z
  PDB

Protein Data Bank: 3FTT

Protein Data Bank: 3V4E

Protein Data Bank: 4DCL

Protein Data Bank: 4EGG

Protein Data Bank: 5U2K

Protein Data Bank: 5V0Z

⊟General

• locus tag: SAUSA300_2492 [new locus tag: SAUSA300_RS13845 ]
• symbol: SAUSA300_2492
• description: acetyltransferase family protein
• length: 199
• theoretical pI: 6.14716
• theoretical MW: 22118.7
• GRAVY: -0.397487

⊟Function

• TIGRFAM:
  colanic acid biosynthesis acetyltransferase WcaF (TIGR04008; EC 2.3.1.-; HMM-score: 70.4)
  sugar O-acyltransferase, sialic acid O-acetyltransferase NeuD family (TIGR03570; HMM-score: 65.9)
  and 13 more
  phosphonate metabolim protein, transferase hexapeptide repeat family (TIGR03308; HMM-score: 54.5)
  2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (TIGR03532; EC 2.3.1.89; HMM-score: 51.6)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 44.5)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 44.5)
  Metabolism Central intermediary metabolism Amino sugars UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 44.5)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase LpxD (TIGR01853; EC 2.3.1.191; HMM-score: 42.7)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase (TIGR01852; EC 2.3.1.129; HMM-score: 36.6)
  Metabolism Amino acid biosynthesis Serine family serine O-acetyltransferase (TIGR01172; EC 2.3.1.30; HMM-score: 33.3)
  colanic acid biosynthesis acetyltransferase WcaB (TIGR04016; EC 2.3.1.-; HMM-score: 32.7)
  non-ribosomal peptide synthetase terminal domain of unknown function (TIGR02353; HMM-score: 30.3)
  Metabolism Energy metabolism Other phenylacetic acid degradation protein PaaY (TIGR02287; HMM-score: 26.7)
  UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR03992; EC 2.3.1.157,2.7.7.23; HMM-score: 15.3)
  Metabolism Amino acid biosynthesis Aspartate family 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (TIGR00965; EC 2.3.1.117; HMM-score: 11.5)
• TheSEED  :
  • Maltose O-acetyltransferase (EC 2.3.1.79)
  Carbohydrates Di- and oligosaccharides Maltose and Maltodextrin Utilization  Maltose O-acetyltransferase (EC 2.3.1.79)
• PFAM:
  no clan defined Mac; Maltose acetyltransferase (PF12464; HMM-score: 56.3)
  HEXAPEP (CL0536) Hexapep_2; Hexapeptide repeat of succinyl-transferase (PF14602; HMM-score: 53.7)
  Hexapep; Bacterial transferase hexapeptide (six repeats) (PF00132; HMM-score: 50.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006282
  • TAT(Tat/SPI): 0.000249
  • LIPO(Sec/SPII): 0.000611
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161930 NCBI
• RefSeq: YP_495126 NCBI
• UniProt: A0A0H2XI29 UniProt

⊟Protein sequence

• MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATNKRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVFIGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAGSVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications