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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2579 [new locus tag: SAUSA300_RS14345 ]
  • pan locus tag?: SAUPAN006375000
  • symbol: SAUSA300_2579
  • pan gene symbol?: —
  • synonym:
  • product: N-acetylmuramoyl-L-alanine amidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2579 [new locus tag: SAUSA300_RS14345 ]
  • symbol: SAUSA300_2579
  • product: N-acetylmuramoyl-L-alanine amidase
  • replicon: chromosome
  • strand: +
  • coordinates: 2795707..2797566
  • length: 1860
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCCTAAAAATAAAATTTTAATTTATTTGCTATCAACTACGCTCGTATTACCTACTTTA
    GTTTCACCTACCGCTTATGCTGATACACCTCAAAAAGATACTACAGCTAAGACAACATCT
    CATGATTCAAAAAAATCTAATGACGATGAAACTTCTAAGGATACTACAAGTAAAGATATT
    GATAAAGCAGACAAAAATAATACAAGTAACCAAGACAATAACGACAAAAAATTTAAAACT
    ATAGACGACAGCACTTCAGACTCTAACAATATCATTGATTTTATTTATAAGAATTTACCA
    CAAACCAATATAAACCAATTGCTAACCAAAAATAAATACGATGATAATTACTCATTAACA
    ACTTTAATCCAAAACTTATTCAATTTAAATTCGGATATTTCTGATTACGAACAACCTCGT
    AATGGCGAAAAGTCAACAAATGATTCGAATAAAAACAGTGACAATAGCATCAAAAATGAC
    ACTGATACGCAATCATCTAAACAAGATAAAGCAGACAATCAAAAAGCACCTAAATCAAAC
    AATACAAAACCAAGTACATCTAATAAGCAACCAAATTCGCCAAAGCCAACACAACCTAAT
    CAATCAAATAGTCAACCAGCAAGTGACGATAAAGCAAATCAAAAATCTTCATCGAAAGAT
    AATCAATCAATGTCAGATTCGGCTTTAGACTCTATTTTGGATCAATACAGTGAAGATGCA
    AAGAAAGCACAAAAAGATTATGCATCTCAATCTAAAAAAGACAAAAATGAAAAATCTAAT
    ACAAAGAATCCACAGTTACCAACACAAGATGAATTGAAACATAAATCTAAACCTGCTCAA
    TCATTCAATAACGATGTTAATCAAAAGGATACACGTGCAACATCATTATTCGAAACAGAT
    CCTAGTATATCTAACAATGATGATAGCGGACAATTTAACGTTGTTGACTCAAAAGATACA
    CGTCAATTTGTCAAATCAATTGCTAAAGATGCACATCGCATTGGTCAAGATAACGATATT
    TATGCGTCTGTCATGATTGCCCAAGCAATCTTAGAATCTGACTCAGGTCGTAGTGCTTTA
    GCTAAGTCACCAAACCATAATTTATTCGGTATCAAAGGTGCTTTTGAAGGGAATTCTGTT
    CCTTTTAACACATTAGAAGCTGATGGTAATCAATTGTATAGTATTAATGCTGGATTCCGA
    AAATATCCAAGCACGAAAGAATCACTAAAAGATTACTCTGACCTTATTAAAAATGGTATT
    GATGGCAATCGAACAATTTATAAACCAACATGGAAATCGGAAGCCGATTCTTATAAAGAT
    GCAACATCACACTTATCTAAAACATATGCTACAGATCCAAACTATGCTAAGAAATTAAAC
    AGTATTATTAAACACTATCAATTAACTCAGTTTGACGATGAACGCATGCCAGATTTAGAT
    AAATATGAACGTTCTATCAAGGATTATGATGATTCATCAGATGAATTCAAACCTTTCCGT
    GAGGTATCTGATAGTATGCCATATCCACATGGCCAATGTACTTGGTACGTATATAACCGT
    ATGAAACAATTTGGTACATCTATCTCAGGTGATTTAGGTGATGCACATAATTGGAATAAT
    CGAGCTCAATACCGTGATTATCAAGTAAGTCATACACCAAAACGTCATGCTGCTGTTGTA
    TTTGAGGCTGGACAATTTGGTGCAGATCAACATTACGGTCATGTAGCATTTGTTGAAAAA
    GTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATT
    TCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
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    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2579 [new locus tag: SAUSA300_RS14345 ]
  • symbol: SAUSA300_2579
  • description: N-acetylmuramoyl-L-alanine amidase
  • length: 619
  • theoretical pI: 6.34359
  • theoretical MW: 69222.8
  • GRAVY: -1.12876

⊟Function[edit | edit source]

  • TIGRFAM:
    flagellar rod assembly protein/muramidase FlgJ (TIGR02541; HMM-score: 81.1)
    and 1 more
    pentapeptide MXKDX repeat protein (TIGR02953; HMM-score: 15.7)
  • TheSEED  :
    • N-acetylmuramoyl-L-alanine amidase, family 4
  • PFAM:
    Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 75.2)
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 66.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helix: 1
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: PTAYADTP
  • SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.99354
    • TAT(Tat/SPI): 0.004521
    • LIPO(Sec/SPII): 0.001352
    • Cleavage Site: CS pos: 27-28. AYA-DT. Pr: 0.9885
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSKKSNDDETSKDTTSKDIDKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQTNINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKNDTDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSSKDNQSMSDSALDSILDQYSEDAKKAQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKSKPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRIGQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQLYSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYATDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPYPHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQFGADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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