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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2603 [new locus tag: SAUSA300_RS14475 ]
  • pan locus tag?: SAUPAN006420000
  • symbol: lip
  • pan gene symbol?: gehA
  • synonym:
  • product: triacylglycerol lipase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2603 [new locus tag: SAUSA300_RS14475 ]
  • symbol: lip
  • product: triacylglycerol lipase
  • replicon: chromosome
  • strand: -
  • coordinates: 2830473..2832515
  • length: 2043
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAAAAGTCAAAATAAGTATAGTATTCGTAAATTTAGTGTAGGTGCATCTTCCATTTTA
    ATAGCTACATTACTATTTTTAAGTGGTGGACAAGCACAAGCAGCTGAGAAGCAAGTGAAT
    ATGGGAAATTCACAGGAGGATACAGTTACAGCACAATCTATTGGGGATCAACAAACTAGG
    GAAAATGCTAATTATCAACGTGAAAACGGTGTTGACGAACAGCAACATACTGAAAATTTA
    ACTAAGAACTTGCATAATGATAAAACAATATCAGAAGAAAATCATCGTAAAACAGATGAT
    TTGAATAAAGATCAACTAAAGGATGATAAAAAATCATCGCTTAATAATAAAAATATTCAA
    CGTGATACAACAAAAAATAACAATGCTAATCCTAGCGATGTAAATCAAGGGTTAGAACAG
    GCTATTAATGATGGTAAACAAAGTAAAGTGGCGTCACAGCAACAGTCAAAAGAGGCAGAT
    AATAGTCAAGATTCAAACGCTAATAACAATCTACCTTCACAAAGTCGAATAAAGGAAGCA
    CCATCATTAAATAAGTTAGATCAAACAAGTCAACGAGAAATTGTTAATGAGACAGAAATA
    GAGAAAGTACAACCACAACAAAATAATCAAGCGAATGATAAAATTACTAACTACAATTTT
    AACAATGAACAAGAAGTGAAACCTCAAAAAGACGAAAAAACACTATCAGTTTCAGATTTA
    AAAAACAATCAAAAATCACCAGTAGAACCAACAAAGGACAATGACAAGAAAAATGGATTA
    AATTTATTAAAAAGTAGTGCAGTAGCAACGTTACCAAACAAAGGGACAAAGGAACTTACT
    GCAAAAGCGAAAGATGATCAAACGAATAAAGTTGCCAAACAAGGGCAGTATAAAAATCAG
    GATCCTATCGTTTTAGTGCATGGTTTCAATGGGTTTACAGATGATATTAATCCTTCAGTG
    TTAGCTCATTATTGGGGCGGTAATAAAATGAACATTCGCCAAGATTTAGAAGAAAATGGT
    TACAAAGCTTATGAAGCAAGTATAAGTGCTTTTGGAAGTAACTATGACCGCGCAGTTGAA
    CTTTATTATTATATCAAAGGCGGTCGTGTAGATTATGGTGCAGCACATGCAGCAAAATAT
    GGACATGAACGTTATGGAAAAACATACGAAGGAATTTACAAAGACTGGAAACCAGGACAG
    AAGGTACACCTAGTTGGACATAGTATGGGCGGTCAAACGATACGTCAACTAGAAGAATTA
    CTGCGTAATGGTAATCGTGAAGAAATAGAGTATCAAAAGAAACATGGTGGCGAAATTTCT
    CCACTATTCAAAGGTAATCATGACAATATGATTTCATCAATTACTACTTTAGGAACACCA
    CATAATGGTACACACGCATCAGATTTAGCTGGTAATGAAGCTTTAGTGAGACAAATCGTA
    TTTGATATCGGTAAAATGTTTGGTAATAAAAATTCAAGAGTAGACTTCGGGTTGGCTCAA
    TGGGGTCTAAAACAGAAGCCAAATGAATCATATATTGATTATGTCAAACGCGTTAAACAA
    TCTAATTTATGGAAATCAAAAGATAATGGATTTTACGATCTGACGCGTGAGGGTGCAACA
    GATTTAAATCGTAAAACGTCGTTGAACCCTAACATTGTGTATAAAACATACACTGGTGAA
    GCAACGCACAAAGCATTAAATAGCGATAGACAAAAAGCAGACTTAAATATGTTTTTCCCA
    TTTGTGATTACTGGTAACTTAATCGGTAAAGCTACTGAAAAAGAATGGCGAGAAAACGAT
    GGTTTAGTATCCGTTATTTCTTCTCAACATCCATTTAATCAAGCTTATACAAAAGCGACA
    GATAAAATTCAAAAAGGCATTTGGCAAGTGACGCCTACAAAACATGATTGGGATCATGTT
    GACTTTGTAGGACAAGACAGTTCTGATACAGTGCGCACAAGAGAAGAATTACAAGATTTT
    TGGCATCATTTAGCAGACGATTTAGTGAAAACTGAAAAGCTGACTGATACTAAGCAAGCA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2043

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2603 [new locus tag: SAUSA300_RS14475 ]
  • symbol: Lip
  • description: triacylglycerol lipase
  • length: 680
  • theoretical pI: 7.66434
  • theoretical MW: 76674.9
  • GRAVY: -1.06456

⊟Function[edit | edit source]

  • reaction:
    EC 3.1.1.3?  ExPASy
    Triacylglycerol lipase Triacylglycerol + H2O = diacylglycerol + a carboxylate
  • TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 52.6)
  • TheSEED  :
    • Triacylglycerol lipase (EC 3.1.1.3)
    Fatty Acids, Lipids, and Isoprenoids Triacylglycerols Triacylglycerol metabolism  Triacylglycerol lipase (EC 3.1.1.3)
  • PFAM:
    no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 44.4)
    and 3 more
    AB_hydrolase (CL0028) PGAP1; PGAP1-like protein (PF07819; HMM-score: 14.6)
    Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 13.4)
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helix: 1
  • LocateP: N-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 2
    • Predicted Cleavage Site: GQAQAAEK
  • SignalP: Signal peptide SP(Sec/SPI) length 34 aa
    • SP(Sec/SPI): 0.983985
    • TAT(Tat/SPI): 0.0051
    • LIPO(Sec/SPII): 0.010084
    • Cleavage Site: CS pos: 34-35. AQA-AE. Pr: 0.9716
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNSQEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLNKDQLKDDKKSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEADNSQDSNANNNLPSQSRIKEAPSLNKLDQTSQREIVNETEIEKVQPQQNNQANDKITNYNFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGTKELTAKAKDDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQDLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGIYKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGNREEIEYQKKHGGEISPLFKGNHDNMISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPNESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKALNSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTKATDKIQKGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKLTDTKQA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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