PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS00245 [old locus tag: SAUSA300_0046 ]
• pan locus tag^?: SAUPAN000511000
• symbol: SAUSA300_RS00245
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS00245 [old locus tag: SAUSA300_0046 ]
• symbol: SAUSA300_RS00245
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 56989..57609
• length: 621
• essential: unknown

⊟Accession numbers

• Location: NC_007793 (56989..57609) NCBI
• BioCyc: SAUSA300_RS00245 BioCyc
• MicrobesOnline: see SAUSA300_0046

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  
  ATGAAATTCACTGAAGTGGAAGTTATCGAACATCTTGTAAAGGCATATAAAGAAGCAGGA
  AAGCCTACTTATCCTCATGAAAATTTATATCGAGGACGTAATCATAGTATTTCAGGTATT
  GGAGAAGACTTGCTGGGTGCTTATTTGATTAGTAGATTGGAAGGTGTCCAAATATTTATT
  GATCAGCCTTTATCTATGATTGATAAATCTTTAAGTACAAGATATCCGGATTTATTAATT
  TGTGAAGATAATGAAATTAAAAATATACTAGAAGTTAAAATGGACTTAGGATATCAAAGA
  AAAGATTTTATAGATTATTGCCGAAAGAAAGAAGAATGGATTTCAAATATCGTAGGAAAA
  CAGTGTGTATTGTCTAGAAAGAGAGAAGACAAAATTCCTATGAATATAGCTGATGATATT
  AAATTTCATGTTGTGATTTACAGTGAAAACAATGGACCGAAGCGGTTTGATGAAGAAATC
  ATGCCTATCGTTAATGAAACATGTCCACATATTGAAGTATATGTCCTAACAAGCGGTCAA
  CACCCTAATTTAGTAAATGTTAATCTTGAAGGTATTAATATTAATAAAGATGAATTTGAA
  ATATTAGTAAATGCGTTATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  621
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS00245 [old locus tag: SAUSA300_0046 ]
• symbol: SAUSA300_RS00245
• description: hypothetical protein
• length: 206
• theoretical pI: 5.02184
• theoretical MW: 23823.2
• GRAVY: -0.358252

⊟Function

• TIGRFAM:
• TheSEED: see SAUSA300_0046
• PFAM:
  ATP-grasp (CL0179) ATPgrasp_TupA; TupA-like ATPgrasp (PF14305; HMM-score: 15.1)
  no clan defined WHAMM-JMY_N; N-terminal of Junction-mediating and WASP homolog-associated (PF15920; HMM-score: 13.2)
  and 1 more
  HeH (CL0306) SAP_RNF34_RFFL; RNF34/RFFL SAP domain (PF23632; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8705
  • Cytoplasmic Membrane Score: 0.0187
  • Cell wall & surface Score: 0.0005
  • Extracellular Score: 0.1103
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00512
  • TAT(Tat/SPI): 0.00025
  • LIPO(Sec/SPII): 0.000875
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446595768 NCBI
• RefSeq: WP_000673114 NCBI
• UniProt: see SAUSA300_0046

⊟Protein sequence

• MKFTEVEVIEHLVKAYKEAGKPTYPHENLYRGRNHSISGIGEDLLGAYLISRLEGVQIFIDQPLSMIDKSLSTRYPDLLICEDNEIKNILEVKMDLGYQRKDFIDYCRKKEEWISNIVGKQCVLSRKREDKIPMNIADDIKFHVVIYSENNGPKRFDEEIMPIVNETCPHIEVYVLTSGQHPNLVNVNLEGININKDEFEILVNAL

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications